Product Details

Uniprot No.

P28340

Target Names

POLD1

Alternative Names

DNA polymerase delta catalytic subunit (EC 2.7.7.7) (EC 3.1.11.-) (DNA polymerase subunit delta p125), POLD1, POLD

Species Reactivity

Human

Immunogen

A synthesized peptide from human POLD1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

POLD1 encodes the catalytic subunit of DNA polymerase delta, a critical enzyme complex responsible for lagging strand synthesis during DNA replication and essential repair pathways including nucleotide excision repair and mismatch repair. Mutations in POLD1 have been linked to hereditary cancer syndromes and genomic instability, making this target particularly relevant for researchers investigating DNA replication fidelity, cancer predisposition, and cellular responses to replicative stress.

This recombinant monoclonal antibody, generated from clone 24B10, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments.

Validation data demonstrates robust performance across multiple applications in human samples. Western blot analysis detects POLD1 across diverse cell lines including A431, K562, HL-60, Jurkat, and THP-1 lysates, with an observed band at approximately 132 kDa. This slight shift from the predicted 124 kDa molecular weight likely reflects post-translational modifications such as phosphorylation, which is known to regulate POLD1 activity during cell cycle progression. Immunohistochemistry staining has been validated in paraffin-embedded human gastric cancer and small intestine tissues using citrate buffer antigen retrieval, while flow cytometry analysis confirms intracellular detection in Jurkat cells following fixation and permeabilization.

The antibody's compatibility with ELISA, western blotting, immunohistochemistry, and flow cytometry provides flexibility for researchers studying POLD1 expression, localization, and regulation across different experimental contexts. This versatility makes it well-suited for investigations into DNA replication mechanisms, cancer biology, and the molecular basis of genomic maintenance disorders.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites.

Gene References into Functions

data suggest that SIRT1 is an oncogenic factor in breast cancer cells and can be involved in the progression of breast cancer by inhibiting p53 and activating POLD1 PMID: 29807012
findings demonstrate a key role of POLD1 and POLD3 in genome stability and S-phase progression revealing RNA-DNA hybrids-dependent effects for POLD3 that might be partly due to its Pol zeta interaction. PMID: 27974823
Mutation in DNA Polymerase III gene is associated with MMR deficiency in cancer. PMID: 28512192
POLD1 played important roles in regulating cell cycle- and DNA replication-related pathways. E2F could upregulate the expression levels of POLD1 by deregulating the methylations of their promoters to promote the relapse of Acute Lymphoblastic Leukemia . PMID: 29768346
DNA polymerase delta catalytic subunit controls noncentrosomal gammaTuRC activity and regulates the organization of Golgi-derived microtubules. PMID: 28916777
POLH & POLK are both able to exchange with PolD1 stalled at repetitive CFS (common fragile sites) sequences. POLD1 synthesis was inhibited by replication stress caused by aphidicolin, preventing any replication past CFS. Importantly, POLH & POLK were still proficient in rescuing this stalled POLD1 synthesis. POLD1 stalling at CFSs allows for free exchange with specialized polymerase that is not driven by PCNA. PMID: 28605669
To our knowledge, the four Valencian families included in the present study are the only families where the POLD1 Leu474Pro mutation has been found. PMID: 28306219
We demonstrated an association between six previously published single nucleotide polymorphisms (rs15869 [ BRCA2], rs1805389 [ LIG4], rs8079544 [ TP53], rs25489 [ XRCC1], rs1673041 [ POLD1], and rs11615 [ ERCC1]) and subsequent CNS tumors in survivors of childhood cancer treated by radiation therapy. PMID: 28976792
The proofreading activity of DNA polymerase delta plays a role in shunting DNA mismatch repair to an EXO1-dependent excision pathway as opposed to directly participating in gap formation via its 3'-5' exonuclease activity. PMID: 28934474
Frameshift mutation in POLD1 gene is associated with mismatch repair-deficiency and Lynch syndrome. PMID: 28218421
the pathogenic role of the POLD1-R689W mutation in the development of the human tumor and emphasize the need to experimentally determine the significance of Poldelta variants present in sporadic tumors. PMID: 28368425
Our work highlights that mutations in different POLD1 domains can lead to phenotypic variability, ranging from dominantly inherited cancer predisposition syndromes, to mild MDPL phenotypes PMID: 28521875
Germline or somatic variants in the POLE/POLD1 were identified in unresolved suspected Lynch syndrome cancers with mismatch repair defect. PMID: 26648449
WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase delta progression across telomeric G-rich repeats, only WRN promotes sequential strand displacement synthesis and FEN1 cleavage. PMID: 27849570
study of complete exonuclease domains of POLE and POLD1 in 529 families characterized by presence of familial or early-onset mismatch repair proficient colorectal cancer, and/or APC-negative and MUTYH-negative polyposis; results widen the phenotypic spectrum of the POLE/POLD1-associated syndrome and identify novel pathogenic variants PMID: 26133394
POLD1 is a central mediator of DNA replication and repair, and it is implied in cancer and other pathologies. (Review) PMID: 27320729
Inactivating POLD1 mutations are associated with colorectal cancer. PMID: 26755646
Mutations in POLE and POLD1 in south east Asia women with grade 3 endometrioid endometrial carcinomas are associated with improved recurrence free survival. PMID: 26748215
POLD1 germline mutations can result in a variably expressed and probably underdiagnosed segmental progeroid syndrome. PMID: 26172944
On the sequence, Escherichia coli oriC plasmid DNA, it was found hPold replicates DNA across the CCG repeats but hPole stalls at the CCG repeats even if the secondary structure is eliminated by a single stranded binding protein. PMID: 26271349
None of the 1188 patients carried the POLD1 p.(Ser478Asn) variant. POLE germline variant carriers are also associated with a microsatellite instable colorectal cancer PMID: 25370038
POLD1 plays important role in the regulation of cell cycle progression and DNA damage repair PMID: 26087769
analysis of phenotype of variants of the essential replicative polymerase-delta carrying missense mutations in its active site PMID: 25241845
POLE and POLD1 mutations explain a fraction of familial CRC and polyposis. Sequencing the proofreading domains of POLE and POLD1 should be considered in routine genetic diagnostics. PMID: 24501277
Germline mutations in the proofreading domains of 2 DNA polymerases (POLE and POLD1) have been associated with a dominantly inherited, highly penetrant syndrome of oligo adenomatous polyposis and early-age-of-diagnosis colorectal and endometrial cancer. PMID: 24509466
POLE and POLD1 mutations are associated with endometrial cancer. PMID: 23528559
a single-codon deletion in POLD1 affecting the polymerase active site causes a markedly different phenotype that includes lipodystrophy. PMID: 23770608
Germline and somatic polymerase epsilon and delta mutations define a new class of hypermutated colorectal and endometrial cancers. PMID: 23447401
the down-regulations of DNA pol delta1 are age-related and have little bearing on diseases and nutritures. DNA pol delta1 has great potential for a new biomarker of aging. PMID: 22915169
Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas. PMID: 23263490
the human lagging strand DNA polymerase delta holoenzyme is distributive PMID: 22942285
reducing expression of individual PRMT7 target DNA repair genes showed that only the catalytic subunit of DNA polymerase, POLD1, was able to resensitize PRMT7 knock-down cells to DNA-damaging agents. PMID: 22761421
Pol switches at replication-blocking lesions occur by the exchange of the Pol delta and Pol zeta catalytic subunits on a preassembled complex of accessory proteins retained on DNA during translesion DNA synthesis. PMID: 22465957
Coronavirus nsp13 and DNA polymerase delta induced DNA replication stress in IBV-infected cells. PMID: 21918226
POLD1 gene mutations are not responsible for the elevated HPRT mutation rates in a colon cancer cell line PMID: 14767555
ubiquitin and ubiquitin-like proteins modify the p66 and p12 subunits of DNA polymerase delta PMID: 16934752
Results suggest that the CDE/CHR-like sequence is an active functional element in the POLD1 promoter, which is important for the cell cycle regulation of the POLD1 gene. PMID: 19557333

Hide All

Involvement in disease

Colorectal cancer 10 (CRCS10); Mandibular hypoplasia, deafness, progeroid features, and lipodystrophy syndrome (MDPL)

Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-B family

Tissue Specificity

Widely expressed, with high levels of expression in heart and lung.

Database Links

HGNC: 9175

OMIM: 174761

KEGG: hsa:5424

STRING: 9606.ENSP00000406046

UniGene: Hs.279413

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Code	CSB-RA207245A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A431 whole cell lysate(30µg), K562 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: POLD1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 124 kDa Observed band size: 132 kDa Exposure time：20s IHC image of CSB-RA207245A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA207245A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA207245A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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POLD1 Recombinant Monoclonal Antibody

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Target Background