Product Details

Uniprot No.

Target Names

Alternative Names

Peptidyl-prolyl cis-trans isomerase D (PPIase D) (EC 5.2.1.8) (40 kDa peptidyl-prolyl cis-trans isomerase) (Cyclophilin-40) (CYP-40) (Cyclophilin-related protein) (Rotamase D), PPID, CYP40 CYPD

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PPID, also known as Cyclophilin-40, is a peptidyl-prolyl cis-trans isomerase that plays essential roles in protein folding and cellular signaling pathways. As a member of the cyclophilin family, PPID functions as a co-chaperone in steroid receptor complexes and has been implicated in various cellular processes including heat shock response and hormone signaling regulation. Its involvement in protein homeostasis makes it a compelling target for researchers investigating chaperone biology, stress responses, and related disease mechanisms.

This recombinant monoclonal antibody, clone 4F2, offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human PPID, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies. Affinity-chromatography purification delivers a reagent ready for sensitive detection applications.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis confirms detection of the expected 41 kDa band in human cell lines including A549 and MCF-7, as well as NIH/3T3 cells and both mouse and rat brain tissue lysates, indicating useful cross-species reactivity for comparative studies. Flow cytometry validation using SH-SY5Y neuroblastoma cells shows clear separation from isotype control, with recommended dilutions ranging from 1:50 to 1:200 providing flexibility for optimization in your specific experimental system.

Whether you are exploring cyclophilin biology in cancer cell models, investigating chaperone networks in neuronal tissue, or characterizing PPID expression across mammalian species, this antibody provides a reliable tool for advancing your research into protein folding machinery and cellular stress responses.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines.; (Microbial infection) May be involved in hepatitis C virus (HCV) replication and release.

Gene References into Functions

Binding of signal transducer and activator of transcription 3 (STAT3) to cyclophilin D (CypD) was important for reducing mitochondrial reactive oxygen species (ROS) production after oxidative stress. PMID: 28351946
Data show that cyclophilin 40 (CyP40) interacts with and dissolves amyloids forming proteins tau and alpha-synuclein aggregates. PMID: 28654636
This review discusses previous studies to provide comprehensive information on the physiological role of cyclophilin D as well as PTP opening in the cell that can be taken into consideration for the development of new PTP inhibitors. [review] PMID: 28378042
The influx of unfolded p53 into the mitochondrial matrix in response to oxidative stress indirectly activates the normally inhibited CypD by displacing it from Trap1 complexes. This activates CypD's isomerase activity. Liberated CypD then isomerizes multiple proteins including p53 (causing p53 aggregation) and the structural components of the mPTP pore, inducing pore opening. PMID: 27515399
The present study is to investigate the role of CypD in regulating the mitochondrial dynamics relevant to oxidative stress induced neuron dysfunctions. PMID: 27993675
CyPD regulates mitochondrial metabolism, and likely cell survival, by promoting more efficient electrons flow through the respiratory chain via increased supercomplex formation PMID: 27916505
cyclophilin D may modify mitochondrial features by inducing the translocation of molecules to the mitochondria through the mechanism associated with cellular energy metabolism PMID: 27864141
The thermodynamics of binding of Cyp-40 to Hsp90 shows remarkable temperature sensitivity in the physiological temperature range. PMID: 26330616
cyclophilin-D protein could increase oxidative stress and cause endothelial cell injury and apoptosis. cyclophilin-D protein is the key factor in reactive oxygen species-induced mitochondrial damage, leading to apoptosis of endothelial cells. PMID: 25966197
Results show that CypD interacts with SPG7 and VDAC to form the mitochondrial permeability transition pore complex (PTP)and its CsA-binding region is necessary for PTP formation. PMID: 26387735
molecular determinants necessary for Cyclophilin D activity regulation and binding to proposed pore constituents thereby regulating the mitochondrial permeability transition pore. PMID: 25445707
Cyp-D silencing down-regulated mitochondrial transcripts initiated from the heavy strand promoter 2 [i.e., NADH dehydrogenase 1 (ND1) by 11-fold; cytochrome oxidase 1 (COX1) by 4-fold; and ATP synthase subunit 6 (ATP6) by 6.5-fold. PMID: 25837584
Cyp40 binds to C2I in vitro and in intact cells, it also interacts with the enzyme components of iota toxin and CDT, suggesting a common principle for this toxin family. PMID: 25058685
The Photorhabdus toxins TccC3 and TccC5 interacted with Hsp90, FKBP51, Cyp40 and CypA, suggesting a role of these host cell factors in translocation and/or refolding of the ADP-ribosyltransferases. PMID: 24138221
The p53/Cyp-D mitochondrial complexation was prevented by CsA or Cyp-D silencing. PMID: 24343341
In summary, the results of the present study provide mechanistic evidence that both apoptosis and programmed necrosis attribute to berberine's cytotoxicity in prostate cancer cells. PMID: 24946211
cisplatin-induced non-apoptotic death requires mitochondria Cyp-D-p53 signaling in pancreatic cancer cells PMID: 23845906
CypD directs mitochondria-to-nuclei inflammatory gene expression in normal and tumor cells PMID: 23303179
These results suggest Cyp-D's critical role in UVB/oxidative stress-induced skin cell death. PMID: 22892127
this is the first report establishing an important role for Cyp40 in lymphoma. PMID: 22681779
Human coronavirus-induced neuronal programmed cell death required cyclophilin d but not caspase 3 caspase 9 activities. PMID: 22013052
adult viable human brain and liver mitochondria possess an active CypD-sensitive mitochondrial permeability transition PMID: 21121808
Data show that show that the molecular chaperone heat shock protein 60 (Hsp60) directly associates with cyclophilin D (CypD). PMID: 20978188
Data show that disruption of FKBP51 and Cyp40 in AI C4-2 cells caused only a small reduction in proliferation. PMID: 20023700
cyclophilin D suppresses apoptotic cell death via a mitochondrial hexokinase II-dependent mechanism in cancer cells PMID: 16551620
CyP40 chaperone function then, is localized within the linker that forms a binding cleft with potential to accommodate non-native substrates PMID: 16650407
Crystal structure of human cyclophilin D in complex with its inhibitor, cyclosporin A at 0.96-A resolution, using a K133I mutant of human CypD PMID: 18076075
CyP40 is found in the cell nucleus after 3-methylchloranthrene treatment and appears to promote the dioxin response element binding form of the AhR/Arnt heterodimer. PMID: 18708059
CypD-mediated mitochondrial permeability transition pore is directly linked to the cellular and synaptic perturbations observed in the pathogenesis of Alzheimer's disease. PMID: 18806802
Cyclophilin D interacts with Bcl2 and exerts an anti-apoptotic effect PMID: 19228691
Cyclophilin D may play a role as a redox sensor in mitochondria of mammalian cells transmitting information on the redox environment to target proteins. PMID: 19735641

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Subcellular Location

Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm.

Protein Families

Cyclophilin-type PPIase family, PPIase D subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 9257

OMIM: 601753

KEGG: hsa:5481

STRING: 9606.ENSP00000303754

UniGene: Hs.183958

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Code	CSB-RA162205A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate(30µg), A549 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Mouse brain tissuelysate(30µg), Rat brain tissuelysate(30µg) All lanes: PPID antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time：2min Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA162205A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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