PREPL Recombinant Monoclonal Antibody

PREPL, or prolyl endopeptidase-like protein, is a serine peptidase that plays essential roles in cellular metabolism and has garnered significant research attention due to its involvement in hypotonia-cystinuria syndrome when deleted alongside neighboring genes. This enzyme's function in processing bioactive peptides makes it a valuable target for researchers investigating metabolic disorders, neurological development, and peptide signaling pathways.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human PREPL, offers the consistency and reliability that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot reproducibility that traditional hybridoma-derived antibodies cannot guarantee. This consistency proves particularly valuable for longitudinal studies or when comparing results across multiple experiments over time.

Validation studies demonstrate robust performance in western blot applications, with successful detection confirmed across multiple sample types including U-251MG glioblastoma cells, HeLa cells, and both mouse and rat brain tissue lysates. The observed band at 84 kDa aligns precisely with the predicted molecular weight, confirming specific target recognition. This cross-species reactivity spanning human, mouse, and rat samples provides flexibility for researchers working with different model systems or conducting comparative studies.

The antibody performs effectively at dilutions ranging from 1:500 to 1:2000 for western blotting, with validation data generated at 1:1000 dilution showing clear signal with minimal background. Additional compatibility with ELISA expands its utility across different experimental platforms. For investigators studying serine peptidase biology, metabolic regulation, or neurodevelopmental processes, this antibody delivers the specificity and reproducibility needed for confident data generation.