Code | CSB-RA161743A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The PRKAG1 recombinant monoclonal antibody is meticulously produced to ensure its exceptional quality and specificity. The process begins by isolating B cells from an immunized animal using the synthesized peptide derived from human PRKAG1 as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The PRKAG1 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. Through transfection, the vector is introduced into host cells to enable the production of the PRKAG1 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for further applications. To ensure its reliability, the antibody undergoes extensive characterization assays, including ELISA and IHC analysis, to validate its specificity and functionality in recognizing human PRKAG1 protein.
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