PRKAG1 Recombinant Monoclonal Antibody

Code CSB-RA161743A0HU
Size US$210
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Image
  • IHC image of CSB-RA161743A0HU diluted at 1:100 and staining in paraffin-embedded human Prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
PRKAG1
Alternative Names
5'-AMP-activated protein kinase subunit gamma-1 (AMPK gamma1) (AMPK subunit gamma-1) (AMPKg), PRKAG1
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human PRKAG1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
5E5
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The PRKAG1 recombinant monoclonal antibody is meticulously produced to ensure its exceptional quality and specificity. The process begins by isolating B cells from an immunized animal using the synthesized peptide derived from human PRKAG1 as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The PRKAG1 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. Through transfection, the vector is introduced into host cells to enable the production of the PRKAG1 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for further applications. To ensure its reliability, the antibody undergoes extensive characterization assays, including ELISA and IHC analysis, to validate its specificity and functionality in recognizing human PRKAG1 protein.

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Target Background

Function
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Gene References into Functions
  1. Data suggest different gamma-isoforms in AMPK can have different effects on enzyme activation; here, activation of AMPK by compound 991 is greater if AMPK contains PRKAG2 versus PRKAG1 or PRKAG3. PMID: 28302767
  2. Pin1 plays an important role in the pathogenic mechanisms underlying impaired glucose and lipid metabolism, functioning as a negative regulator of AMPK PMID: 26276391
  3. Letter: report PRKAG1 frameshift mutations in colorectal cancer. PMID: 25267969
  4. Electrogenic phosphate transport in sodium/phosphate cotransporter NaPi-IIa-expressing Xenopus oocytes is markedly decreased by the coexpression of constitutively active AMPKgammaR70Q, but not of AMPK(alphaK45R). PMID: 24356547
  5. PRKAG1 interacts with DSCAM through its gamma subunit, and netrin-1 activates AMPK phosphorylation in cortical neurons. PMID: 23479427
  6. Conclude that cTnI phosphorylation by AMPK may represent a novel mechanism of regulation of cardiac function. PMID: 22456184
  7. Inhibition of the KCa3.1 channels by AMP-activated protein kinase in human airway epithelial cells. PMID: 19052260

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Protein Families
5'-AMP-activated protein kinase gamma subunit family
Database Links

HGNC: 9385

OMIM: 602742

KEGG: hsa:5571

STRING: 9606.ENSP00000323867

UniGene: Hs.530862

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