PSMA3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA140307A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), A549 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg), K562 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Raw264.7 whole cell lysate(30µg) All lanes: PSMA3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time：1min20s IHC image of CSB-RA140307A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA140307A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing BxPC-3 cells stained with CSB-RA140307A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P25788

Target Names

PSMA3

Alternative Names

Proteasome subunit alpha type-3 (EC 3.4.25.1) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8) (Proteasome component C8), PSMA3, HC8 PSC8

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human PSMA3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PSMA3, also known as proteasome subunit alpha type-3 or HC8, serves as a core component of the 20S proteasome complex responsible for protein degradation through the ubiquitin-proteasome pathway. This essential cellular machinery regulates numerous critical processes including cell cycle progression, signal transduction, and the removal of misfolded or damaged proteins. Dysregulation of proteasome function has been implicated in various pathological conditions, making PSMA3 a valuable target for researchers investigating protein homeostasis, cancer biology, and cellular stress responses.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human PSMA3, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies.

Validation data demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects a clean band at the predicted 28 kDa molecular weight across an extensive panel of human cell lines including HT-29, A549, MCF-7, HeLa, PC-3, K562, and HL-60, as well as mouse cell lines NIH/3T3 and Raw264.7, confirming cross-species reactivity with both human and mouse samples. Immunohistochemistry validation in human liver cancer and small intestine tissues shows reliable tissue staining, while flow cytometry analysis in BxPC-3 cells demonstrates clear positive signal separation from isotype controls.

This versatile antibody supports researchers studying proteasome biology, protein quality control mechanisms, and therapeutic strategies targeting the ubiquitin-proteasome system in oncology and beyond.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Gene References into Functions

High PSMA3 expression is associated with cholangiocarcinoma. PMID: 28560424
Our results provide evidence on new T1DM-susceptible loci in the PSMA3, PSMA6 and PSMC6 proteasome genes and give a new insight into the T1DM pathogenesis PMID: 26661414
Evidence of a sex-specific association of PSMA3 genetic variants with subtypes of juvenile idiopathic arthritis. PMID: 24875235
Protein interaction between Ambn and Psma3 can facilitate redistribution of ameloblastin domains within forming enamel. PMID: 26070558
A combination of two-dimensional gel electrophoresis (2D-GE) and tandem mass-spectrometry revealed a large number of PSMA3-bound proteins that are involved in various aspects of mRNA metabolism, including splicing. PMID: 22079093
Serum 20S proteasome concentration and percentage of lymphocytic apoptosis predicted survival and patient prognosis in critically ill patients. PMID: 21108816
Plasminogen activator inhibitor type 1 interacts with alpha3 subunit of proteasome and modulates its activity. PMID: 21135093
Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis PMID: 14674694
EBNA3C binds to human the C8/alpha7 subunit of the 20S proteasome; degraded by purified 20S proteasomes in vitro but, found to be very stable and apparently turned over at a very low rate in B cells infected latently with EBV PMID: 15831937
The authors propose that the full-length HCV F protein as well as the F protein initiating from codon 26 is degraded by an ubiquitin-independent pathway that is mediated by the proteasome subunit alpha3. PMID: 18971267

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase T1A family

Database Links

HGNC: 9532

OMIM: 176843

KEGG: hsa:5684

STRING: 9606.ENSP00000216455

UniGene: Hs.558799

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