Phospho-PSMA3 (Ser250) Antibody

Datasheet

Code	CSB-PA264880
Size	US$360
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Image	Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Proteasome α3 (Phospho-Ser250) antibody. The lane on the right is treated with the synthesized peptide.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMA3 Polyclonal antibody

Uniprot No.

P25788

Target Names

PSMA3

Alternative Names

HC8 antibody; Macropain subunit C8 antibody; MGC12306 antibody; MGC32631 antibody; Multicatalytic endopeptidase complex subunit C8 antibody; Proteasome (prosome macropain) subunit alpha type 3 antibody; Proteasome alpha 3 subunit antibody; Proteasome component C8 antibody; Proteasome subunit alpha type 3 antibody; Proteasome subunit alpha type-3 antibody; Proteasome subunit C8 antibody; PSA3_HUMAN antibody; PSC8 antibody; psmA3 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Peptide sequence around phosphorylation site of serine 250 (D-E-S(p)-D-D) derived from Human Proteasome α3.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Gene References into Functions

High PSMA3 expression is associated with cholangiocarcinoma. PMID: 28560424
Our results provide evidence on new T1DM-susceptible loci in the PSMA3, PSMA6 and PSMC6 proteasome genes and give a new insight into the T1DM pathogenesis PMID: 26661414
Evidence of a sex-specific association of PSMA3 genetic variants with subtypes of juvenile idiopathic arthritis. PMID: 24875235
Protein interaction between Ambn and Psma3 can facilitate redistribution of ameloblastin domains within forming enamel. PMID: 26070558
A combination of two-dimensional gel electrophoresis (2D-GE) and tandem mass-spectrometry revealed a large number of PSMA3-bound proteins that are involved in various aspects of mRNA metabolism, including splicing. PMID: 22079093
Serum 20S proteasome concentration and percentage of lymphocytic apoptosis predicted survival and patient prognosis in critically ill patients. PMID: 21108816
Plasminogen activator inhibitor type 1 interacts with alpha3 subunit of proteasome and modulates its activity. PMID: 21135093
Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis PMID: 14674694
EBNA3C binds to human the C8/alpha7 subunit of the 20S proteasome; degraded by purified 20S proteasomes in vitro but, found to be very stable and apparently turned over at a very low rate in B cells infected latently with EBV PMID: 15831937
The authors propose that the full-length HCV F protein as well as the F protein initiating from codon 26 is degraded by an ubiquitin-independent pathway that is mediated by the proteasome subunit alpha3. PMID: 18971267

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase T1A family

Database Links

HGNC: 9532

OMIM: 176843

KEGG: hsa:5684

STRING: 9606.ENSP00000216455

UniGene: Hs.558799

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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