Code | CSB-RA018986A0HU |
Size | US$210 |
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The production of the PTGS2 recombinant monoclonal antibody involves the application of DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human PTGS2 to elicit an immune response and isolate B cells. Positive B cells are then carefully selected and subjected to screening and single clone identification. The light and heavy chains of the PTGS2 antibody are subsequently amplified using PCR and inserted into a plasmid vector. This recombinant vector is then introduced into a host cell line for antibody expression. The PTGS2 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This highly specific antibody is capable of recognizing human PTGS2 protein and is well-suited for ELISA.
The PTGS2 protein, also known as COX-2, is an enzyme that plays a key role in the production of prostaglandins, which are involved in inflammation and pain responses in the body. In cells, the PTGS2 protein is involved in the conversion of arachidonic acid to prostaglandin H2, which is then converted into various other prostaglandins by downstream enzymes. The PTGS2 protein is induced in response to inflammatory stimuli such as cytokines, growth factors, and stress. In addition to its role in inflammation and pain, the PTGS2 protein has also been implicated in other physiological processes, such as fever, angiogenesis, and tumor growth.
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