Product Details

Uniprot No.

Target Names

Alternative Names

FLJ36528 antibody; KIAA0235 antibody; MGC138251 antibody; MGC138253 antibody; PUM 2 antibody; PUM2 antibody; PUM2_HUMAN antibody; PUMH 2 antibody; PUMH2 antibody; Pumilio (Drosphila) homolog 2 antibody; Pumilio homolog 2 (Drosophila) antibody; Pumilio homolog 2 antibody; Pumilio-2 antibody; Pumilio2 antibody; PUML2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PUM2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PUM2, or Pumilio homolog 2, is an RNA-binding protein that plays a central role in post-transcriptional gene regulation. As a member of the PUF family of translational repressors, PUM2 binds specific sequences in the 3' untranslated regions of target mRNAs, influencing their stability and translation. This regulatory function positions PUM2 as a key player in diverse biological processes, including stem cell maintenance, neuronal function, and germ cell development, making it a compelling target for researchers investigating epigenetics and nuclear signaling pathways.

This recombinant monoclonal antibody, clone 16B2, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human PUM2, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. The rabbit IgG format, combined with affinity chromatography purification, delivers a reagent optimized for reliable performance.

Validation studies confirm robust utility in flow cytometry applications, with recommended dilutions ranging from 1:50 to 1:200. Testing in HeLa cells demonstrated clear positive signal separation from isotype control, with the antibody performing effectively at 1 microgram per million cells following fixation and permeabilization. This intracellular staining protocol, using formaldehyde fixation and Triton X-100 permeabilization, provides a validated starting point for your own experimental optimization.

For researchers exploring RNA-binding protein networks, translational control mechanisms, or the broader landscape of post-transcriptional regulation in human cells, this antibody provides a dependable tool for flow cytometric analysis of PUM2 expression.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level.

Gene References into Functions

PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs PMID: 30084199
A recent paper by the Mendell group identifies NORAD, a novel lncRNA that is regulated in response to DNA damage and plays a key role in maintaining genome integrity by modulating the activity the RNA binding proteins PUM2 and PUM1. PMID: 27157388
results reveal a novel regulatory pathway, underscoring a previously unknown and interconnected key role of PUM1/2 and FOXP1 in regulating normal hematopoietic stem/progenitor cell and leukemic cell growth. PMID: 28232582
The study shows that pseudouridine and N(6)-methyladenosine weaken the binding of the human single-stranded RNA binding protein Pumilio 2 (hPUM2) to its consensus motif. PMID: 28138061
Pumilios (including PUM1 and PUM2) are RNA-binding proteins with Puf domains made up of 8 poorly conserved Puf repeats, 3 helix bundles arranged in rainbow architecture, where each repeat recognizes a single base of the RNA-binding sequence. [REVIEW] PMID: 26517885
Identification of NORAD-interacting proteins revealed that this lncRNA functions as a multivalent binding platform for PUM proteins, with the capacity to sequester a significant fraction of the total cellular pool of PUM1 and PUM2. Studies reveal unanticipated roles for a lncRNA and PUMILIO proteins in the maintenance of genomic stability. PMID: 26724866
PUM2 does not repress differentiation of adipose-derived stem cells (ASCs) but rather is involved in the positive control of ASCs division and proliferation. PMID: 21649561
human Pumilio homologs Pum 1 and Pum 2 repress the translation of E2F3 by binding to the E2F3 3' untranslated region (UTR) and also enhance the activity of multiple E2F3 targeting microRNAs (miRNAs) PMID: 22345517
Mammalian PUM2-Ago-eEF1A inhibited translation of nonadenylated and polyadenylated reporter mRNAs in vitro. PMID: 22231398
in mitosis, PUM2 physically associates with Aurora-A to ensure enough active Aurora-A at centrosomes for mitotic entry PMID: 21589936
Three distinct modes of target RNA binding by PUM2 around the fifth mRNA base were observed. PMID: 21397187
PUM2 as a component of conserved cellular machinery that may be required for germ cell development. PMID: 12511597
PUM2 and DAZL, are capable of binding the same mRNA sequences in the 3'UTR of human SDAD1 mRNA. PMID: 15607425
P2P-R expression may be translationally regulated by PUM2 PMID: 15617101
PUMILIO mediated translational regulation may be universally used in developmental processes of the human body. PMID: 16967088
Variation in the PUMILIO2 gene is very low and it seems improbable that mutations of this gene significantly contribute to male infertility in humans. PMID: 17154300
Data found that PUMILIO2 interacts also with SNAPIN, a modulator of SNARE complex assembly, which is involved in vesicle trafficking. PMID: 19168546

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Subcellular Location

Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region.

Tissue Specificity

Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach E

Database Links

HGNC: 14958

OMIM: 607205

KEGG: hsa:23369

STRING: 9606.ENSP00000338173

UniGene: Hs.467824

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Code	CSB-RA066680A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing Hela cells stained with CSB-RA066680A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PUM2 Recombinant Monoclonal Antibody

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Target Background