Code | CSB-RA010833A78phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
In the quest to manufacture the phospho-HSPB1 (S78) recombinant monoclonal antibody, the initial phase involves the retrieval of genes encoding the HSPB1 antibody from rabbits previously exposed to a synthesized peptide originating from the human HSPB1 protein phosphorylated at S78. These antibody genes are then adeptly integrated into specialized expression vectors. Subsequently, the genetically modified vectors are thoughtfully introduced into host suspension cells, where they are diligently cultivated to stimulate the production and secretion of antibodies. Following this growth phase, the phospho-HSPB1 (S78) recombinant monoclonal antibody is subjected to a rigorous purification process employing affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Lastly, the antibody's efficacy is comprehensively assessed through a diverse range of assays, encompassing ELISA, WB, and IHC tests, thereby confirming its ability to interact effectively with the human HSPB1 protein phosphorylated at S78.
Phosphorylation of HSPB1 at S78 is a critical regulatory mechanism that allows cells to respond to stress, maintain protein quality control, and promote cell survival. Dysregulation of this phosphorylation event can have significant implications in various diseases, including cancer and neurodegenerative disorders.
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