Code | CSB-RA010833A82phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The DNA sequence coding for the pS82-HSPB1 monoclonal antibody produced from the animals with the phosphopeptide corresponding to the resides surrounding the Ser 82 of human HSPB1 immunization was cloned into the expression vector, which was further transfected into a cell line for in vitro expression. The product is the recombinant phospho-HSPB1-S82 monoclonal antibody. It specifically targets the human HSPB1 phosphorylated at Ser 82 residue. It belongs to the rabbit IgG. The affinity-chromatography purification method was used to purify this phosphorylated HSPB1 antibody. ELISA, WB, and IHC have been tested for this HSPB1 antibody.
HSPB1, an abundant molecular chaperone, is found in striated muscle and is phosphorylated in response to several stimuli, including mechanical stress. In response to stretch, HSPB1 is phosphorylated in cells and tissues, causing translocation to the Z-discs and to locations of elevated traction force within the cytoskeleton, implying that phosphorylation may initiate or regulate its interactions with mechanosensitive clients like FLNC at these sites. MAPKAPK2/3 phosphorylates HSPB1 at serine sites 15, 78, and 82 in the N-terminal region of the protein, outside of the alpha-crystallin domain.
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