Phospho-HSPB1 (S82) Antibody

Datasheet
Code CSB-RA010833A82phHU
Size US$350
Uniprot No. P04792
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
    All lanes:Phospho-HSPB1 antibody at 0.73μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 27 KDa
    Observed band size: 27 KDa

  • IHC image of CSB-RA010833A82phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen A synthesized peptide derived from Human Phospho-HSPB1 (S82)
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Immunogen Species Homo sapiens (Human)
Research Area Signal Transduction
Gene Names HSPB1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state
Involvement in disease Charcot-Marie-Tooth disease 2F (CMT2F); Neuronopathy, distal hereditary motor, 2B (HMN2B)
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle
Protein Families Small heat shock protein (HSP20) family
Tissue Specificity Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal f
Database Links

HGNC: 5246

OMIM: 602195

KEGG: hsa:3315

STRING: 9606.ENSP00000248553

UniGene: Hs.520973

Pathway MAPK signaling pathway
VEGF signaling pathway

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