RCC1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA589725A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HeLa whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg), SW620 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), K562 whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: RCC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 50 kDa Exposure time: 1min IHC image of CSB-RA589725A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA589725A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA589725A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P18754

Target Names

RCC1

Alternative Names

Cell cycle regulatory protein antibody; CHC 1 antibody; CHC1 antibody; Chromosome condensation 1 antibody; Chromosome condensation protein 1 antibody; Guanine nucleotide releasing protein antibody; HERC2 antibody; Ran GEF antibody; RanGEF antibody; RCC 1 antibody; RCC1 antibody; RCC1 I antibody; RCC1_HUMAN antibody; Regulator of chromosome condensation 1 antibody; Regulator of chromosome condensation antibody; SHEP1 antibody; SNHG3 RCC1 antibody; SNHG3 RCC1 readthrough transcript antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RCC1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2A4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RCC1, the Regulator of Chromosome Condensation 1, serves as the essential guanine nucleotide exchange factor for the small GTPase Ran, establishing the RanGTP gradient that orchestrates fundamental cellular processes including nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. This central role in cell cycle regulation makes RCC1 a compelling target for researchers investigating chromosome dynamics, cell division mechanisms, and cancer biology.

This recombinant monoclonal antibody, clone 2A4, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation across multiple platforms demonstrates the versatility of this antibody for diverse experimental approaches. Western blot analysis confirms robust detection of RCC1 across an extensive panel of human cell lines including HeLa, HepG2, Jurkat, HEK293, SW620, HT-29, K562, and A549, with the observed band at approximately 50 kDa slightly exceeding the predicted 45 kDa molecular weight—a difference likely attributable to post-translational modifications such as phosphorylation or glycosylation that regulate RCC1 function. Immunohistochemistry validation in human colorectal cancer and testis tissue sections demonstrates clear nuclear localization patterns consistent with RCC1's chromatin-associated function. Flow cytometry analysis in Jurkat cells further extends the utility of this antibody for single-cell studies.

Whether investigating mitotic checkpoint mechanisms, exploring nucleocytoplasmic transport dynamics, or examining RCC1 expression patterns in cancer progression, this antibody provides a reliable tool for advancing your research into fundamental cell biology and disease mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.

Gene References into Functions

This study provides first evidence for a mutation in RCC1 as a breast cancer susceptibility allele PMID: 29363114
Importin alpha3 facilitates the nuclear transport of the Ran guanine nucleotide exchange factor RCC1. PMID: 29042532
this study has demonstrated that EBNA1 and RCC1 closely associate on the chromosomes during metaphase, suggesting an essential role for the interaction during this phase, perhaps in tethering EBNA1 to mitotic chromosomes. PMID: 28284242
RCC1 expression enables the proliferation of cells that sustain DNA damage. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. PMID: 26864624
RCC1 expression is frequently lost in poorly differentiated gastric cell lines and gastric carcinoma tissues. Loss of RCC1 expression is correlated with tumor differentiation and depth of invasion. PMID: 26823742
stress can disrupt the Ran gradients through RCC1-dependent and RCC1-independent mechanisms PMID: 25452301
PI3Kbeta regulates the nuclear envelope through upstream regulation of RCC1 and Ran PMID: 25348717
RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase. PMID: 23536659
RCC1 can induce bipolar spindle formation. Remarkably, RCC1 beads oscillate within spindles from pole to pole, a behavior that could be converted to a more typical, stable association by the addition of a kinesin together with RCC1. PMID: 22215983
discovery of the first alpha-N-methyltransferase, which we named N-terminal RCC1 methyltransferase (NRMT). PMID: 20668449
The authors find that RCC1 uses a conformationally flexible loop region we have termed the switchback loop in addition to its N-terminal tail to bind to the nucleosome. PMID: 20347844
RanBP3 associates with the Ran-specific guanine nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1). PMID: 11932251
These results indicate that the generation of Ran-GTP in the vicinity of chromosomes by RCC1 is important for the fidelity of mitotic spindle assembly in human cells. PMID: 12194828
RCC1 release from chromatin in vitro requires a factor(s) distinct from, or in addition to, Ran PMID: 14565978
phosphorylation of the NLS of RCC1 is required to prevent the binding of importin alpha and beta to RCC1, thereby allowing RCC1 to couple RanGTP production to chromosome binding PMID: 15014043
This 30 kb deletion contains the exons coding for the RCC1-like domain of RPGR. It is the first report of a macrodeletion that spans the entire RCC1-like domain of RPGR in X-linked retinitis pigmentosa patients PMID: 16052169
HSV-1 replication is blocked in cell line tsBN2 encoding a temperature sensitive RCC1 protein. PMID: 17251553
Propose bimodal attachment mechanism for RCC1 in which the tail promotes stable RCC1 association with chromatin through DNA binding in an alpha-N-methylation-dependent manner. PMID: 17435751
These results show that human RCC1 isoforms have distinct chromatin binding properties, different molecular interactions, and are selectively regulated by phosphorylation, as determined by their different NTRs. PMID: 17855385
chromosome 1p36 was established to have linkage for adhd. PMID: 18439570
A review of the function and structure of RCC1 and RCC1 superfamily genes and proteins. PMID: 18442486
reduced binding affinity of RCC1 to the mitotic chromosome, which led to a reduction of RanGTP level. PMID: 18712773
The N-terminal tail of RCC1 is essential for association with DNA but inhibits histone binding. PMID: 18762580
Therefore, we propose that RCC1 reads the histone code created by caspase-activated Mst1 to initiate apoptosis by reducing the level of RanGTP in the nucleus. PMID: 19060893

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Subcellular Location

Nucleus. Chromosome. Cytoplasm.

Database Links

HGNC: 1913

OMIM: 179710

KEGG: hsa:1104

UniGene: Hs.469723

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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