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RDX Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA098472A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate(30µg),HT-29 whole cell lysate(30µg),Hela whole cell lysate(30µg),k562 whole cell lysate(30µg),U-251MG whole cell lysate(30µg),NIH/3T3 whole cell lysate(30µg)
    All lanes: Radixin antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 69 kDa
    Observed band size: 69,80 kDa
    Exposure time：2min
  • Overlay Peak curve showing HepG2 cells stained with CSB-RA098472A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P35241
Target Names
RDX
Alternative Names
CB567 antibody; CG12537 antibody; DFNB24 antibody; ESP10 antibody; Hh-induced MATH and BTB domain-containing protein antibody; HIB antibody; Moesin-B antibody; Protein roadkill antibody; RADI_HUMAN antibody; Radixin antibody; RDX antibody
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human RDX
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
15A6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Radixin belongs to the ERM (ezrin-radixin-moesin) protein family, serving as a critical membrane-cytoskeleton linker that regulates cell adhesion, migration, and signal transduction. This protein plays essential roles in organizing membrane domains and anchoring the actin cytoskeleton to the plasma membrane, making it a key target for researchers investigating cytoskeletal dynamics, cell motility, and membrane organization in both normal physiology and disease states.

This recombinant rabbit monoclonal antibody (clone 15A6) offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human radixin and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your results remain comparable across extended studies.

Validation across multiple platforms demonstrates the flexibility of this reagent. Western blot analysis confirms robust detection in diverse human cell lines including HEK293, HT-29, HeLa, K562, and U-251MG, as well as mouse NIH/3T3 cells, supporting cross-species reactivity for human and mouse samples. The observed bands at 69 kDa align with the predicted molecular weight, while an additional band at approximately 80 kDa likely reflects post-translational modifications such as glycosylation or phosphorylation, which are well-documented for ERM family proteins. Flow cytometry validation in HepG2 cells demonstrates clear positive signal separation from isotype controls, confirming utility for intracellular protein detection in fixed and permeabilized samples.

Whether investigating cytoskeletal remodeling, cell migration mechanisms, or membrane-associated signaling complexes, this antibody provides researchers with a reliable tool for exploring radixin biology across multiple experimental approaches.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Gene References into Functions
  1. CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041
  2. The results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site. PMID: 28889652
  3. Radixin knockdown suppresses the metastasis of SGC-7901 cells in vitro by up-regulation of E-cadherin. The NF-kappaB/snail pathway contributes to the regulation of E-cadherin in response to depletion of radixin. PMID: 27855404
  4. Phospho-Ezrin/Radixin/Moesin (ERM) inhibit cell adhesion, and therefore, dephosphorylation of ERM proteins is essential for cell adhesion.Phospho-ERM induce formation and/or maintenance of spherical cell shape. PMID: 26555866
  5. These studies identify Akt2 as a critical kinase that regulates radixin phosphorylation and leads to Mrp-2 translocation and function. PMID: 26500117
  6. Intracellular sphingosine kinase 2-derived sphingosine-1-phosphate mediates epidermal growth factor-induced ezrin-radixin-moesin phosphorylation and cancer cell invasion. PMID: 26209696
  7. High Radixin expression is associated with glioblastoma. PMID: 25520109
  8. Ezrin, radixin and moesin are unlikely targets for autoantibodies in demyelinating neuropathies. PMID: 25286001
  9. These data suggest an association between RDX polymorphisms and the clinical features of RA patients, particularly the ESR PMID: 24854655
  10. Radixin was identified as a target gene of miR-196a/-196b. Elevated miR-196a/-196b expression in GC cells led to reduced radixin protein levels and vice versa. PMID: 24933454
  11. This study finding have implications concerning the importance of concomitant radixin upregulation in tumor progression and poor prognosis of patients with gliomas. PMID: 24380686
  12. important role of ezrin-moesin-radixin proteins during HCV infection at the postentry level and highlight possible novel targets for HCV treatment. PMID: 23703860
  13. ERM proteins play important differential roles in the thrombin-induced modulation of EC permeability, with moesin promoting barrier dysfunction and radixin opposing it. PMID: 23729486
  14. Data suggest that P-glycoprotein associate with the F-actin cytoskeleton through ezrin/radixin/moesin (ERM) in CCR9/CCL25 induced multidrug resistance of acute T-lymphocytic leukemia (T-ALL) cells. PMID: 23326330
  15. Control of adipogenesis by ezrin, radixin and moesin-dependent biomechanics remodeling. PMID: 23116763
  16. Data show that silencing of radixin (RDX) phenocopied the effects of miR-409 overexpression, whereas restoration of RDX in miR-409-overexpressed gastric cancer (GC) cells reversed the suppressive effects of miR-409. PMID: 22179828
  17. Ezrin/radixin/moesin are required for the purinergic P2X7 receptor (P2X7R)-dependent processing of the amyloid precursor protein. PMID: 22891241
  18. results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through involvement of down-regulation of TSP-1 and E-cadherin expression. PMID: 22631643
  19. role of CCM3 and ezrin/radixin/moesin family of proteins in cell's response to oxidative stress PMID: 22291017
  20. Ano1, ezrin, and moesin/radixin colocalize apically in salivary gland epithelial cells, and overexpression of moesin and Ano1 in HEK cells alters the subcellular localization of both proteins PMID: 22685202
  21. Radixin selectively modulates the expression and function of MRP2 in human gastric adenocarcinoma. PMID: 22469515
  22. MiR-31 targeted radixin predominantly via inhibition of protein translation instead of degradation of mRNA. PMID: 22089331
  23. results indicate that ezrin influences the expression of P-gp at the translational level, whereas radixin is involved in membrane localization of P-gp in HepG2 cells PMID: 21837648
  24. The ERM (ezrin, radixin, moesin) proteins are novel scaffolds at the level of SOS activity control, which is relevant for both normal Ras function and dysfunction known to occur in several human cancers. PMID: 22132106
  25. Ezrin, radixin, and moesin are phosphorylated in response to 2-methoxyestradiol and modulate endothelial hyperpermeability. PMID: 21659656
  26. the ezrin, radixin and moesin proteins differentially modulate sphingosine 1 phosphate-induced alterations in lung endoethlial cell cytoskeleton and permeability PMID: 21864676
  27. This study provides the novel evidence that increased phosphorylation of Ezrin/radixin/moesin proteins may contribute to proliferation of rheumatoid fibroblast-like synoviocytes. PMID: 21278069
  28. Dysregulation of the ezrin/radixin/moesin-RAGE complex might be an important step in rearrangement of the actin cytoskeleton during proinflammatory cytokine-induced epithelial-mesenchymal transition of human alveolar epithelial cells. PMID: 21278261
  29. ezrin, radixin and moesin play similar roles in the tumor cell metastatic potential and their roles of upregulating the expression of E-cadherin may be important in tumor progression PMID: 21352885
  30. Polar distribution of radixin suggest a role in promoting formation and plasticity of membrane surface projections and also suggest that radixin is an organizer and regulator of Mrp-2 and cell polarity in hepatocytes. PMID: 21160029
  31. the identification of radixin as a scaffolding unit for both cAMP effectors, Epac and PKA PMID: 21047789
  32. Results demonstrate a new regulatory mechanism of ezrin-radixin-moesin phosphorylation by sphingolipids with opposing actions of ceramide and sphingosine 1-phosphate. PMID: 20679347
  33. Data suggest that ezrin-radixin-moesin proteins are involved in the spatial regulation of Epac1 and cooperate with cAMP- and Rap-mediated signaling to regulate adhesion to the extracellular matrix. PMID: 20855527
  34. amino-terminal domains of the ezrin, radixin, and moesin (ERM) proteins bind advanced glycation end products and have a role in diabetes PMID: 12734202
  35. Disrupted localization of radixin and MRP2 supports the concept that radixin contributes to the canalicular localization of MRP2. PMID: 14568249
  36. ezrin/radixin/moesin proteins are recruited by NHE1 Na+/H+ exchanger and have roles in regulating Akt-dependent cell survival PMID: 15096511
  37. All three ERM family members can localise to the nucleus; a specific nuclear localisation sequence, which is conserved and functional in all ERM family members, is identified, implying specific regulated nuclear import. PMID: 15149851
  38. results suggest that ezrin-radixin-moesin proteins are required for microvillar positioning of L-selectin and that this is important both for leukocyte tethering and L-selectin shedding PMID: 15178693
  39. Ezrin, radixin and moesin are ADP-ribosylated by Pseudomonas aeruginosa ExoS PMID: 15252013
  40. Conformational regulation of radixin protein function occurs by association of the FERM and C-terminal domains, whereby the membrane- and actin-binding activities are mutually suppressed [review] PMID: 15313460
  41. Expression of ExoS in HeLa cells led to a loss of phosphorylation of Ezrin/radixin/moesin proteins that was dependent upon the expression of ADP-ribosyltransferase activity. PMID: 16889625
  42. 2 mutant alleles of RDX in 2 consanguineous families associated with neurosensory hearing loss; sequence analysis of RDX from original DFNB24 family revealed a c.463C>T transition substitution predicted to truncate radixin in the FERM domain PMID: 17226784
  43. increased CD44, ezrin, radixin, and moesin phosphorylation represents a key molecular abnormality that guides T cell adhesion and migration in SLE patients. PMID: 17237445
  44. RDX was differentially expressed in sclerotic hippocampi compared to non-sclerotic ones. PMID: 17515952
  45. Report disturbed colocalization of multidrug resistance protein 2 and radixin in human cholestatic liver diseases. PMID: 17725603
  46. Radixin and ezrin play similar roles in the apical membrane localization of ABCC2 (MRP2) and their expression level and subcellular distribution are important factors in the regulation of ABCC2 (MRP2) at the post-transcriptional level. PMID: 17825285
  47. the ezrin, radixin, and moesin proteins function as positive regulators of infection by X4-tropic HIV-1 PMID: 18295815
  48. Unlike ezrin and moesin, radixin (a member of the ezrin-radixin-moesin protein family)is not expressed in Jurkat cells, nor is it involved in apoptotic signaling following Fas receptor triggering in these cells. PMID: 18941185
  49. No aspects of T cell receptor signaling uniquely require transgenic ezrin or moesin; instead, T cell activation appears to depend on net ezrin, radixin, and moesin (ERM)protein expression, while ezrin and moesin function together. PMID: 19124745
  50. In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin PMID: 19129194

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Involvement in disease
Deafness, autosomal recessive, 24 (DFNB24)
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cell projection, microvillus. Note=Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
Database Links

HGNC: 9944

OMIM: 179410

KEGG: hsa:5962

STRING: 9606.ENSP00000342830

UniGene: Hs.263671

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