Product Details

Uniprot No.

Target Names

RNF2

Alternative Names

E3 ubiquitin-protein ligase RING2 (EC 2.3.2.27) (Huntingtin-interacting protein 2-interacting protein 3) (HIP2-interacting protein 3) (Protein DinG) (RING finger protein 1B) (RING1b) (RING finger protein 2) (RING finger protein BAP-1) (RING-type E3 ubiquitin transferase RING2), RNF2, BAP1 DING HIPI3 RING1B

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RNF2, also known as RING1B, serves as a critical E3 ubiquitin-protein ligase and core component of the Polycomb Repressive Complex 1 (PRC1). This enzyme catalyzes the monoubiquitination of histone H2A at lysine 119, a modification essential for transcriptional silencing and the maintenance of cellular identity during development. RNF2's involvement in chromatin remodeling and gene regulation makes it a compelling target for researchers investigating epigenetic mechanisms, stem cell biology, and cancer progression.

This recombinant monoclonal antibody, clone 4D9, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a sequence-defined rabbit IgG format, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The defined molecular composition ensures that your results remain comparable across experiments, whether you're establishing baseline data or building on previous findings.

Validation studies demonstrate reliable performance across multiple experimental platforms. Western blot analysis confirms specific detection of RNF2 at the predicted 38 kDa molecular weight across diverse human cell lines including HeLa, K562, and SH-SY5Y, as well as mouse NIH/3T3 cells, providing flexibility for researchers working with different model systems. Flow cytometry validation using Raji cells shows clear separation between specific staining and isotype control, confirming utility for intracellular protein quantification in fixed and permeabilized samples.

Whether your research focuses on Polycomb-mediated gene silencing, histone modification dynamics, or the role of ubiquitination in cellular differentiation and disease states, this antibody provides a dependable tool for investigating RNF2 biology with confidence in your results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation.

Gene References into Functions

these data indicate that RING1B and EZH2 repress the innate inflammatory cutaneous squamous cell carcinoma function and impair tumor immunosurveillance PMID: 29394319
Observations demonstrated that Plk1 directly interacts with RNF2 and degrades RNF2 via the ubiquitindependent degradation pathway. RNF2 may be used as a new target for mitotic regulation and tumorigenesis. PMID: 29565459
These data suggest that RNF2 is an important upstream negative regulator of SIK1 and that restoration of SIK1 levels induced by loss of RNF2 inhibited HCC cell growth and promoted apoptosis, which may represent a promising therapeutic strategy for HCC treatment. PMID: 27911266
Study found that prostate cancer (PCa) tissues have high RNF2 expression which is positively correlated with tumor grade indicating that RNF2 may have oncogenic function in PCa. PMID: 28029659
Our findings identify RING1B as a trait of the cell-of-origin in Ewing sarcoma PMID: 27317769
Study detected RNF2 expression to be overexpressed in esophageal carcinoma cell lines and was associated with the poor prognosis of esophageal carcinoma patients. Data also reflect its important role in the growth of esophageal carcinoma cells by shRNA silencing of RNF2 expression. PMID: 26936624
overexpression of RNF2, as examined by immunohistochemical analysis, might serve as a novel prognostic biomarker and potential therapeutic target for urothelial carcinoma of the bladder patients. PMID: 26869491
Our findings support the notion that epigenetic regulators, such as RNF2, directly and functionally control powerful gene networks that are vital in multiple cancer processes. PMID: 26450788
O-GlcNAc modifies and regulates an essential epigenetic tool, RING1B, which may contribute to human embryonic stem cells pluripotency maintenance and differentiation. PMID: 26100231
The enzymatic activity of BMI1 and RING1B were evaluated and compared. PMID: 26151332
Bmi1 and possibly RNF2 may be attractive biomarkers of gemcitabine resistance in the context of RRM1 expression. PMID: 24614341
combinatorial silencing along with Ezh2 depleted epigenetically modified H2- and H3-histones and inhibited pancreatic cancer cell growth in vitro and in vivo (xenograft model) PMID: 25431952
BAP1 deficiency causes increased expression of FoxK2 target genes in a Ring1B-Bmi1-dependent manner PMID: 25451922
Authors describe a critical role for the polycomb group protein Ring1b in nonhomologous end-joining (NHEJ)-mediated end-to-end chromosome fusions. PMID: 24813883
Ring1B and the SNAG-associated chromatin modifier EZH2 formed distinct protein complexes with Snail and that EZH2 was required for Snail-Ring1A/B recruitment to the target promoter. PMID: 24903147
The acidic patch functions within the nucleosome as nucleosomes containing a mutated acidic patch exhibit defective H2A/H2AXub by RNF168 and RING1B/BMI1 in vitro PMID: 24603765
Data indicate that RING finger protein 2 (RNF2) knockdown can increase the sensitivity of U87 cells to X ray radiation. PMID: 24796740
Expressed the isolated N-terminal region of Ring1B, N-Ring1B, comprising the first 221 residues of the 334-residue-long Ring1B and found that the N-Ring1B is a well-folded, monomeric fragment, with native-like structure which unfolds irreversibly. PMID: 24284202
BMI-1 and RING1B expression was enhanced with the development of embryos in early pregnancy. PMID: 23727134
knockdown of RNF2 significantly inhibits both cell proliferation and colony formation and induces apoptosis in cancer cells PMID: 23318437
Ring2 may effect the DNA repair through other pathways but not through the expressions of NER protein. PMID: 23712474
Results indicate that RNF2 is an E3 ligase for p53 degradation in selective cells, implicating RNF2 as a therapeutic target to restore tumor suppression through p53 in certain tumor cells. PMID: 23319651
the reduction of LPS-mediated IL-8 promoter activation was not related to de novo X-DING-CD4 protein synthesis, but depended on function of the exogenous X-DING-CD4 protein. PMID: 22031506
These data provide evidence that the X-DING-CD4 gene contributes to early cellular protection from HIV infection in some individuals and this protection depends solely on the unique genetic regulation of the host. PMID: 22042911
The organism reacts to HIV-infection by an overexpression of DING proteins. PMID: 22427948
Crystallography of a complex of the Bmi1/Ring1b RING-RING heterodimer & UbcH5c shows that UbcH5c interacts with Ring1b only. Bmi1/Ring1b interacts with nucleosomal DNA & an acidic patch on histone H4 to achieve specific monoubiquitination of H2A. PMID: 21772249
identification of genes regulated by ring finger protein 2 PMID: 21347701
Polycomb Group protein targeting to different chromatin locations relies, in part, on binding partners of the C-terminal domain of RING1B that are diverse in sequence and structure. PMID: 20696397
Ring1B plays an important role in the induction of T helper (Th)2 cell-driven allergic airway inflammation through the control of Bim-dependent apoptosis of effector T helper (Th)2 cells in vivo. PMID: 20237291
polycomb protein Ring1B regulation by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome PMID: 20351251
The human PcG protein dinG interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription. PMID: 11865070
The E3 ubiquitin ligase RNF2 might have a dual function: facilitating the ubiquitination of its target substrates and recruiting the substrates to the proteasome. PMID: 15773819
Bmi-1-Ring1B complex stabilizes the interaction of the ubiquitin ligase complex. PMID: 16714294
Ring1B and Bmi1 are degraded by an exogenous E3, independent of their RING domain. The RING domains of both proteins mediate their association and subsequent stabilization. PMID: 17157253
caspases-3 and caspase-9 play novel roles in transcription by regulating polycomb protein function through direct cleaving of Ring1B. PMID: 17379327
With prohibitin, regulates the activity of E2F1 transcription factor via dual pathways. PMID: 17873902
Results show that a phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin. PMID: 17936708
Data show that the presence of flexible regions could allow C-terminal region of RING1B to bind a variety of different factors, ultimately recruiting RING1B and its associated PcG proteins to different genomic loci. PMID: 18616292
a novel mechanism by which RNF2 and PHB2 modulate the CP2-mediated transcriptional pathway. PMID: 18629613
Altered mRNA expression is associated with therapy failure and death in patients with multiple types of cancer. PMID: 15931389

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Subcellular Location

Nucleus. Cytoplasm. Chromosome.

Database Links

HGNC: 10061

OMIM: 608985

KEGG: hsa:6045

STRING: 9606.ENSP00000356480

UniGene: Hs.591490

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Code	CSB-RA123809A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg) All lanes: RING2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time：2min Overlay Peak curve showing Raji cells stained with CSB-RA123809A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RNF2 Recombinant Monoclonal Antibody

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Target Background