Product Details

Uniprot No.

Target Names

Alternative Names

Deleted in U twenty twenty antibody; DUTT 1 antibody; DUTT1 antibody; FLJ21882 antibody; H Robo 1 antibody; H-Robo-1 antibody; hRobo 1 antibody; Robo 1 antibody; Robo1 antibody; ROBO1_HUMAN antibody; Roundabout 1 antibody; Roundabout axon guidance receptor homolog 1 antibody; Roundabout homolog 1 antibody; Roundabout homolog1 precurser antibody; Roundabout1 antibody; SAX 3 antibody; SAX3 antibody

Species Reactivity

Human

Immunogen

Recombinant Human ROBO1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

22D5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ROBO1, also known as Roundabout homolog 1, serves as a critical transmembrane receptor in the evolutionarily conserved Slit-Robo signaling pathway. This axon guidance receptor plays fundamental roles in neuronal migration, axon pathfinding, and cellular positioning during development. Beyond its established functions in neuroscience, ROBO1 has emerged as a significant target in cancer research, where its altered expression patterns have been implicated in tumor progression and metastatic behavior, making it a compelling molecule for investigators exploring both developmental biology and oncology.

This recombinant monoclonal antibody, clone 22D5, offers the reproducibility and consistency that demanding experimental workflows require. Generated against recombinant human ROBO1 protein and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your results remain comparable across extended studies.

Validation testing demonstrates robust performance across multiple detection platforms. Immunohistochemistry studies confirm clear staining in paraffin-embedded human brain tissue and human gastric cancer samples at 1:30 dilution using citrate buffer antigen retrieval, providing researchers with confidence when examining both normal and pathological specimens. Immunofluorescence applications have been validated in A549 and HeLa cell lines at 1:20 dilution, revealing cellular localization patterns suitable for subcellular distribution studies. Flow cytometry analysis of HeLa cells demonstrates effective surface detection with clear separation from isotype controls, enabling quantitative assessment of ROBO1 expression levels in cell populations.

Whether investigating axon guidance mechanisms, characterizing tumor samples, or profiling cell surface receptor expression, this antibody delivers the versatility and reliability needed for meaningful discoveries in neuroscience and cancer research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.

Gene References into Functions

Robo1 expression might counteract migration and also radiation-induced migration of glioblastoma cells, a process that might be connected to mesenchymal-epithelial transition. PMID: 29864155
the expression of hsa_circRNA0054633 has a protective effect against high glucoseinduced endothelial cell dysfunction by targeting ROBO1 and HO1. PMID: 29693114
Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. PMID: 28738960
miR-218 inhibits human lung adenocarcinoma cell migration and invasion via the suppression of Ecop and Robo1 expression PMID: 28936884
we show that variants in ROBO1 are associated with CHD involving septal defects and tetralogy of Fallot. PMID: 28592524
SLIT2/ROBO1 signalling may regulate trophoblast differentiation and invasion causing restricting beta human chorionic gonadotrophin (beta-hCG) production, shallow trophoblast invasion and inhibiting placental angiogenesis in missed and threatened miscarriage during the first trimester. PMID: 28485101
The findings indicate that the migration of human neural progenitor cells from the fetal subventricular zone to the olfactory bulb is partially regulated by the Slit2-Robo1 axis. PMID: 28406573
These findings offer direct evidence supporting ROBO1-callosum association in humans and also provide valuable insight into the functions of ROBO1 and the gene-to-brain mechanisms that underlie human reading. PMID: 28240421
Differential expression levels and methylation status of ROBO1 in mantle cell lymphoma and chronic lymphocytic leukaemia. PMID: 28004534
The alteration of Slit2 and Robo1 expression in the retinas of diabetic rats and patients with proliferative diabetic retinopathy suggests a role for the Slit-Robo signal in the various stages diabetic retinopathy. PMID: 28973045
Results demonstrate that human ROBO1 may be involved in the regulation of the structure and connectivity of posterior part of corpus callosum. PMID: 27240594
ROBO1 gene mutation is responsible for the development of pituitary stalk interruption syndrome. PMID: 28402530
human placental multipotent mesenchymal stromal cell express Slit2 and both Robo1 and Robo4 are present in human umbilical vein endothelial cells. PMID: 26745454
Slit2-Robo1 signaling promoted the adhesion, invasion and migration of tongue carcinoma cells by upregulating the expression levels of MMP2 and MMP9 and, downregulating the expression of E-cadherin. PMID: 27431199
High Slit2 expression is associated with glioma. PMID: 27916173
ROBO1 mediated the inhibitory effect of miR-218 on angiogenesis in gastric cancer. PMID: 28323002
Results indicate the importance of SLIT2-ROBO1-CDC42 signaling pathway in predicting tumor progression. PMID: 27659325
We postulate that Robo1 promotes tumor invasion partly by the upregulation of MMP2 after activation of PI3K/Akt signaling pathway. Notably, Slit2 knockdown caused the upregulation of Robo1 expression both at the mRNA and protein levels. Thus, the stimulatory effects of Slit2 knockdown on tumor progression can be ascribed, at least in part, to the upregulation of Robo1 and its positive role in tumor progression. PMID: 27176045
ROBO1 deletion in a putative transcriptional regulatory region PMID: 26427657
Over-expression of miR-218 in glioma cells may inhibit the proliferation and tumorigenicity through targeting Robo1, suggesting that miR-218 could be a potential target for developing therapies in treating glioma. PMID: 26889813
Significantly increased serum Slit2 levels and hepatic expression of Slit2 and Robo1 were observed in patients with liver fibrosis. PMID: 26264936
expression pattern in extravillous trophoblasts associated with the remodeling events of the tubal pregnancy PMID: 26282852
our findings indicated that Slit2/Robo1 axis possibly be regarded as a significant clinical parameter for predicting brain metastasis in breast cancer patients. PMID: 26400100
miR-29a markedly inhibits the protein expression of Robo1 in mesenchymal stem cells. PMID: 26252416
A SLIT2/ROBO1 signaling circuit as a key regulatory mechanism. PMID: 26975850
These results suggest that Slit2/Robo1 binding exerts an effect on cell migration, which is negatively regulated by Robo4, and Robo1 may function by interacting with CdGAP in HUVECs. PMID: 26713366
ROBO1 somatic mutation is associated with myelodysplastic syndrome progression. Overexpression of ROBO1 produces anti-proliferative and pro-apoptotic effects in leukaemia cells. However, this effect was lost in ROBO mutants. PMID: 26608094
Robo1 promoted cell division cycle 42 (Cdc42) expression in HUVECs, and a distorted actin cytoskeleton in HUVECs was observed when Robo1 expression was suppressed. In conclusion, Robo1 promoted angiogenesis in HCC mediated by Cdc42. PMID: 26022159
these studies demonstrate that miR-219-5p inhibited cancer cell growth and invasion by direct targeting ROBO1, implicating miR-219-5p as an attractive candidate for cancer therapy. PMID: 26081620
Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/beta-catenin pathway. PMID: 25605242
miR29a inhibits cell migration and invasion in breast cancer cells, at least in part, by directly targeting Robo1. PMID: 25955714
In this review, we summarize recent findings demonstrating that the neuronal guidance cues, Slit and Roundabout (Robo), prevent the migration of multiple leukocyte subsets towards diverse inflammatory chemoattractants. PMID: 24777535
Inactivation of SLIT2 and/or ROBO1 is one of the early events in development of dysplastic lesions of head and neck and has prognostic importance. PMID: 25465073
identify two separate binding sites for heparin interaction with Robo1: one binding site at the previously identified site for heparin dp8 and a second binding site at the N terminus of Robo1 that is disordered in the x-ray crystal structure PMID: 25752613
prognostic implications of SLIT and ROBO1 expression in gallbladder cancer PMID: 24777813
Data show that ubiquitin specific peptidase 33 (USP33) mediates nerve tissue proteins Slit-Robo signaling in lung cancer cell migration. PMID: 24981056
ROBO1 was a functional target of miRNA-218's downstream pathway involving in cell invasion and migration of pancreatic cancer. PMID: 25010661
ROBO1 contributs to the deficits in developmental dyslexia and its correlated phenotypes. PMID: 24430574
Family-based analysis shows association of SNPs in ROBO1 with reading diasbilities. PMID: 24612512
Lower expression of ROBO1 is associated with prostate cancer disease progression. PMID: 24752651
Full-length Robo1 is present almost exclusively as a dimer; parallel studies demonstrate the biological activity of Slit2 and its interaction with Robo1. PMID: 24673457
Frameshift mutations of ROBO1 and ROBO2 genes and alteration of ROBO2 expression in gastric and colorectal cancers suggest that both genes might play roles in the pathogenesis of both cancers. PMID: 24247621
Downregulation of miRNA-218 and upregulation of ROBO-1 were first demonstrated in pancreatic cancer. PMID: 23733161
Low Robo1 expression was associated with cell proliferation and migration in ICC and was one of the adverse prognostic factors in patients with these tumors. PMID: 23953227
No genetic association of ROBO1 with developmental dyslexia was found in Indian population. PMID: 23954868
Data indicate that slit2N alters the localization and binding of Robo1 to WASp and LSP1 in HIV-1-gp120-treated immature dendritic cells (iDCs). PMID: 23119100
breast cancer cell migration and invasion was promoted when miRNA- 218 was significantly down regulated, in the way of up-regulation of Robo1. PMID: 22898079
data suggests the importance of abrogation of SLIT2-ROBO1 and SLIT2-ROBO2 interactions in the initiation and progression of CACX and also for early diagnosis and prognosis of the disease PMID: 22719878
we report that the tumorigenic potential of breast cancer cells is determined by an interaction between the Robo1 receptor and its ligand Slit2 PMID: 22826604
Robo1 expression correlates negatively with invasive ductal carcinoma brain metastasis, and correlates positively with the age and prognosis of IDC patients. PMID: 21875486

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass membrane protein.

Protein Families

Immunoglobulin superfamily, ROBO family

Tissue Specificity

Widely expressed, with exception of kidney.

Database Links

HGNC: 10249

OMIM: 602430

KEGG: hsa:6091

STRING: 9606.ENSP00000420321

UniGene: Hs.744218

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Code	CSB-RA896760MA1HU
Size	US$210
	Order now
Image	IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110⁶cells) used under the same conditions. Acquisition of >10,025 events was performed.
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ROBO1 Recombinant Monoclonal Antibody

Product Details

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Target Background