Product Details

Uniprot No.

Target Names

Alternative Names

Regulatory-associated protein of mTOR (Raptor) (p150 target of rapamycin (TOR)-scaffold protein), RPTOR, KIAA1303 RAPTOR

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Raptor

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RPTOR, commonly known as Raptor, serves as an essential scaffold protein within the mTORC1 complex, where it recruits substrates for phosphorylation and regulates cellular responses to nutrient availability, energy status, and growth factor signaling. As a critical mediator of mTOR pathway activity, Raptor influences fundamental processes including protein synthesis, autophagy, and cell growth, making it a compelling target for researchers investigating metabolic regulation, cancer biology, and cellular stress responses.

This recombinant monoclonal antibody, generated from clone 1B10 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The affinity-purified IgG format provides clean, specific detection without the variability often encountered with polyclonal preparations.

Validation studies demonstrate reliable performance in immunohistochemistry applications, with successful staining confirmed in paraffin-embedded human small intestine and kidney tissues using citrate buffer antigen retrieval at pH 6.0. Working dilutions of 1:50 to 1:200 provide flexibility to optimize signal intensity based on tissue type and detection system sensitivity. The antibody has also been validated for ELISA applications, expanding its utility across different experimental platforms.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody is well-suited for laboratories investigating mTOR signaling dynamics, nutrient sensing pathways, and the broader landscape of epigenetic and nuclear signaling events that govern cellular fate decisions.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD).

Gene References into Functions

Study shows that USP9X deubiquitylating enzyme maintains RAPTOR protein levels, mTORC1 signalling and proliferation in neural progenitors. USP9X is the first deubiquitylating enzyme shown to stabilize RAPTOR. PMID: 28341829
Results suggest that RPTOR mediates, at least partially, the resistance to EGFR inhibition in triple-negative breast cancer cells. PMID: 29344641
data reveal that RAPTOR up-regulation contributes to PI3K-mTOR inhibitor resistance, and suggest that RAPTOR expression should be included in the pharmacodynamic assessment of mTOR kinase inhibitor trials PMID: 29389967
Hypomethylation of CpG sites in RPTOR, MGRN1 and RAPSN in blood is associated with breast cancer. PMID: 27577081
RPTOR (regulatory associated protein of mTOR, complex 1) is a novel target of miR-155 in CF lung epithelial cells. The suppression of RPTOR expression and subsequent activation of TGF-beta signaling resulted in the induction of fibrosis by elevating connective tissue growth factor (CTGF) abundance in CF lung epithelial cells. PMID: 27284727
Data show that mTOR protein forms a complex with Raptor and estrogen receptor-alpha (ERalpha). PMID: 26522726
Up-regulation of mTORC1 via raptor by aldosterone is a critical pathobiologic mechanism that controls pulmonary artery smooth muscle cell survival to promote hypertrophic vascular remodeling and pulmonary arterial hypertension. PMID: 27006450
Hgh mTOR activity and Rictor overexpression could be markers of a bad prognosis. Combined phosphoprotein and Rictor/Raptor expression evaluation revealed even stronger statistical correlation with prognosis. PMID: 27729429
revealed more than 170 NFAT-associated proteins, half of which are involved in transcriptional regulation. Among them are many hitherto unknown interaction partners of NFATc1 and NFATc2 in T cells, such as Raptor, CHEK1, CREB1, RUNX1, SATB1, Ikaros, and Helios. PMID: 27637333
In this study we began by validating the expression of four main mTOR pathway components, mTOR, DEPTOR, rictor and raptor, at gene and protein level in in vitro models of endometrioid (MDAH2774) and clear cell (SKOV3) ovarian cancer PMID: 27211906
Our results demonstrate for the first time that the expression quantitative trait loci of RPTOR, rs7502563, is susceptible to glioma PMID: 26361958
The positive regulation of mTORC1 activity by NPRL2 is mediated through NPRL2 interaction with Raptor. PMID: 26582740
RPTOR was not associated with bipolar disorder or schizophrenia. PMID: 25053281
microRNA-210 and raptor are involved in mithramycin-mediated erythroid differentiation of K562 cells and participate to the fine-tuning and control of gamma-globin gene expression in erythroid precursor cells. PMID: 25849663
the expressions of Beclin1, Raptor, and Rictor are related to the development and progression of colorectal carcinoma and multidrug resistance. PMID: 26363527
Common genetic variation in RPTOR is associated with overweight/obesity but does not discernibly contribute to either essential hypertension or isolated systolic hypertension in the Hawaiian population studied. PMID: 25249372
Studied the impact of SNPs in/near UCP3 and RPTOR on obesity-related traits. PMID: 25533680
phosphorylating the T-loop Akt residue Thr(308) by PDK1 requires Raptor of the mTORC1 complex as a platform or scaffold protein. PMID: 24516643
suggest no connection of RPTOR variants with psoriasis or its subphenotypes. PMID: 24005976
peptides that encompass the Raptor cross-linking region of 4E-BP1 inhibit cross-linking and interaction of 4E-BP1 with Raptor. PMID: 24403073
Amino acids promote mTORC1 activation without altering Rag GTP charging. PMID: 24337580
There is a significant downregulation of mTOR, DEPTOR, and Raptor in preterm labouring myometria when compared to non-pregnant tissues taken from the same area. PMID: 23541542
Raptor polymorphism is associated with gastric cancer. PMID: 23423739
Histolopathological and clinical information including tumour stage, invasion characteristic and endocrine status were analysed against the gene transcript expression of mTOR, RAPTOR and RICTOR. PMID: 23898069
Single nucleotide polymorphisms in RPTOR gene is associated with obesity. PMID: 23563607
An essential role of mTORC1 in autophagy inhibition in cell-free system in which, membrane association of Barkor/Atg14(L), a specific autophagosome-binding protein, is suppressed by cytosol from nutrient-rich medium, is shown. PMID: 22258093
show that E2F1 is capable of inducing growth by regulating mTORC1 activity PMID: 21283628
Adiponectin induces vascular smooth muscle cell differentiation via repression of mammalian target of rapamycin complex 1 and FoxO4 PMID: 21454807
results suggest that GRp58/ERp57 is involved in the assembly of mTORC1 and positively regulates mTORC1 signaling at the cytosol and the cytosolic side of the endoplasmic reticulum PMID: 21321085
found that osmotic stress activates mTORC1 kinase activity in a JNK-dependent manner. Our findings suggest that the molecular link between JNK and Raptor is a potential mechanism by which stress regulates the mTORC1 signaling pathway PMID: 22493283
an important role for ICK in modulating the activity of mTORC1 through phosphorylation of Raptor Thr-908. PMID: 22356909
Phosphorylated forms of Raptor are significantly increased in mitotic cells. Raptor resides at the nucleoplasm and nucleolus of interphase cells and remains targeted to nucleolar organizer regions during mitosis. PMID: 21900751
ULK1 contributes to mTORC1 inhibition through hindrance of substrate docking to Raptor. PMID: 21460630
a previously unrecognized function of miR-21 that is the reciprocal regulation of PTEN levels and Akt/TORC1 activity that mediate critical pathologic features of diabetic kidney disease PMID: 21613227
The study identified raptor as a substrate for p44/42 MAPK. PMID: 21325048
Data show that a SNP (rs11868112) 26 kb upstream to the transcription start site of RPTOR exhibiting the strongest association with temperature variables. PMID: 21060808
a novel regulatory mechanism by which mitogenic and oncogenic activation of the Ras/MAPK pathway promotes mTOR signaling PMID: 21071439
there are two parallel cell-survival pathways in prostate cancer cells: a strong Akt-independent, but rapamycin-sensitive pathway downstream of mTORC1, and an AR-dependent pathway downstream of mTORC2 and Akt, that is stimulated by mTORC1 inhibition PMID: 18776922
Study demonstrates that Serine 696 and Threonine 706 represent two key sites in raptor phosphorylation during mitosis, and that the mitotic cyclin-dependent kinase cdc2/CDK1 is the kinase responsible for phosphorylating these sites. PMID: 20169205
These data suggest that the raptor/mTORC1 pathway may play a role in increased IRES-dependent mRNA translation during mitosis and in rapamycin insensitivity. PMID: 20439490
S2448 phosphorylated mTOR binds to both raptor and rictor. PMID: 19145465
Complex raptor phosphorylation functions as a biochemical rheostat that modulates mTORC1 signaling in accordance with environmental cues. PMID: 19864431
Raptor is a missing component of the mTOR pathway that through its association with mTOR regulates cell size in response to nutrient levels PMID: 12150925
raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo. PMID: 12150926
raptor binds to p70S6k and 4E-BP1 through their respective TOS (conserved TOR signaling) motifs. PMID: 12604610
A redox-sensitive mechanism regulates the phosphorylation of the raptor-mTOR effector S6K1, the interaction between raptor and mTOR, and the kinase activity of the raptor-mTOR complex. PMID: 16183647
Raptor directly binds to and serves as a scaffold for mTOR-mediated phosphorylation of IRS-1 on Ser636/639. PMID: 16354680
These studies suggest that, through serine phosphorylation, Raptor-mTOR and S6K1 promote the depletion of IRS1 from specific intracellular pools in pathological states of insulin and IGF-I resistance and in lesions associated with tuberous sclerosis. PMID: 16914728
These data suggest that, during HCMV infection, the rictor- and raptor-containing complexes are modified such that their substrate specificities and rapamycin sensitivities are altered. PMID: 16959881
ASCT2 silencing inhibits mTORC1 (mTOR/raptor) signaling and leads to growth repression, followed by enhanced survival signaling via mTORC2 (mTOR/rictor) and apoptosis of hepatoma cells. PMID: 17329400

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Subcellular Location

Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.

Protein Families

WD repeat RAPTOR family

Tissue Specificity

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

Database Links

HGNC: 30287

OMIM: 607130

KEGG: hsa:57521

STRING: 9606.ENSP00000307272

UniGene: Hs.133044

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Code	CSB-RA581950A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA581950A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA581950A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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RPTOR Recombinant Monoclonal Antibody

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