Product Details

Uniprot No.

Target Names

Alternative Names

85 kDa lysosomal membrane sialoglycoprotein antibody; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody; AMRF antibody; CD36 antibody; CD36 antigen (collagen type I receptor; thrombospondin receptor)-like 2 (lysosomal integral membrane protein II) antibody; CD36 antigen antibody; CD36 antigen-like 2 antibody; CD36L2 antibody; EPM4 antibody; HLGP85 antibody; LGP85 antibody; LIMP 2 antibody; LIMP II antibody; LIMP2 antibody; LIMPII antibody; Lysosomal integral membrane protein II antibody; Lysosome membrane protein 2 antibody; Lysosome membrane protein II antibody; OTTHUMP00000160590 antibody; OTTHUMP00000219176 antibody; Scarb2 antibody; Scavenger receptor class B member 2 antibody; Scavenger receptor class B; member 2 antibody; SCRB2_HUMAN antibody; SR BII antibody; SRBII antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human SCARB2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SCARB2, also known as lysosomal integral membrane protein 2 (LIMP-2), serves as a critical receptor mediating the transport of beta-glucocerebrosidase to lysosomes and functions as a cellular entry receptor for certain enteroviruses. This protein plays significant roles in lysosomal biogenesis and membrane organization, with mutations linked to action myoclonus-renal failure syndrome and implications for cardiovascular research. Understanding SCARB2 expression and localization provides valuable insights into lysosomal function and associated pathological conditions.

This recombinant monoclonal antibody, clone 12F9, offers the consistency and reproducibility that demanding research protocols require. Generated against a synthetic peptide derived from human SCARB2 and produced using recombinant technology in rabbit host, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies.

Extensive validation confirms reliable performance across multiple platforms. Western blot analysis demonstrates robust detection in diverse human cell lines including HeLa, MCF7, HEK293, PC-3, A549, U251, and COLO205, with cross-species reactivity confirmed in mouse and rat brain tissue lysates. The observed band at 77 kDa, higher than the predicted 54 kDa molecular weight, reflects the extensive glycosylation characteristic of this lysosomal sialoglycoprotein. Immunohistochemistry validation in paraffin-embedded human liver tissue and colorectal cancer specimens demonstrates clear staining at 1:100 dilution, while flow cytometry analysis of A431 cells shows distinct positive population shifts compared to isotype controls.

Whether investigating lysosomal biology, viral entry mechanisms, or cardiovascular-related pathways, this antibody provides the flexibility to move seamlessly between western blotting, immunohistochemistry, and flow cytometry applications within your research workflow.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.; (Microbial infection) Acts as a receptor for enterovirus 71.

Gene References into Functions

Crystal structure of a LIMP-2 luminal domain dimer with bound cholesterol and phosphatidylcholine is described. Binding of these lipids alters LIMP-2 from functioning as a glucocerebrosidase-binding monomer toward a dimeric state that preferentially binds anionic phosphatidylserine over neutral phosphatidylcholine. PMID: 29199275
The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection. PMID: 28447294
The findings identify SR-BII as a functional SAA receptor that mediates SAA uptake and contributes to its proinflammatory signaling via the MAPK-mediated signaling pathways. PMID: 28423002
In this paper we provide an updated overview of the clinical and genetic features of SCARB2-related PME and on the functions of the LIMP2 protein [review] PMID: 27582254
neutrophils are able to drive a macrophage activation that would regulate the increase in LIMP-2 expression during the early phase of Cer-induced acute pancreatitis PMID: 26794464
Findings suggested that STX1B rs4889603, FAM47E rs6812193 and SCARB2 rs6825004 do not confer a significant risk for Parkinson's disease PMID: 26224037
LIMPII was increased greater than twofold in urinary microvesicles obtained from patients with idiopathic membranous nephropathy compared to microvesicles of patients with idiopathic focal segmental glomerulosclerosis and normal controls. PMID: 26304790
Although hrGCase cellular uptake is independent of LIMP-2, its trafficking to the lysosomes is mediated by this receptor. PMID: 26018676
SNCA and SCARB2 loci are also associated with dementia with Lewy bodies, after a study-wise Bonferroni correction, although these have a different association profile than the associations reported for the same loci in Parkinson's. PMID: 24973356
a novel mutation in SCARB2 as a cause of progressive myoclonus epilepsy in China PMID: 24620919
SCARB2 regulates TLR9-dependent IFN-I production of plasmacytoid dendritic cells PMID: 25862818
Disease-causing cathepsin-F mutants fail to cleave LIMP-2. Our findings provide evidence that LIMP-2 represents an in vivo substrate of cathepsin-F with relevance for understanding the pathophysiology of type-B-Kufs-disease. PMID: 25576872
Data indicate that scavenger receptor SCARB2 triggers uncoating of human enterovirus 71 (EV71) under low pH conditions. PMID: 24986489
SCARB2 and PSGL-1 in human gastrointestinal tract, lung, and brain tissues correlated with the distribution of pathological changes seen in EV71 infection. PMID: 24997419
In LIMP-2-deficient brains a significant reduction in GC activity led to lipid storage, disturbed autophagic/lysosomal function, and alpha-synuclein accumulation. PMID: 25316793
The LIMP-2/SCARB2 binding sequences for enterovirus 71 and GCase are not similar, indicating that LIMP-2/SCARB2 may have multiple or overlapping binding sites with differing specificities. PMID: 25202012
A novel SCARB2 mutation was indicated by reduced Beta-glucocerebrosidase activity in progressive myoclonus epilepsy. PMID: 24485911
Mutations in LIMP-2, the specific receptor for glucocerebrosidase that is missing in Gaucher disease patients, substantially contribute to the pathology and heterogeneity of the disease. (Review) PMID: 24389070
study of two Japanese siblings with late-onset progressive myoclonus epilepsy without renal failure having a novel homozygous SCARB2 mutation PMID: 23325613
Human SCARB2-transgenic mice are a useful model for assessing anti-EV71 medications. PMID: 23451246
Results suggest that this hSCARB2 transgenic mouse could represent a useful animal model for the study of enterovirus 71 (EV71) infection. PMID: 23959904
identified no novel exonic variants in SCARB2 but confirmed the association between SNP rs6812193 and Parkinson's disease. PMID: 23408458
SCARB2 is implicated in the lysosomal pathway recently associated with Parkinson disease (PD) pathogenesis. The rs6812193 polymorphism doesn't increase susceptibility to PD in the Greek population. PMID: 23473716
These results indicated that SCARB2 is capable of viral binding, viral internalization, and viral uncoating and that the low infection efficiency of L-PSGL1 cells is due to the inability of PSGL1 to induce viral uncoating. PMID: 23302872
Lysosomal storage disorders are associated with deficiencies of alternative lysosomal receptors LIMPII and sortilin and/or of their cargos. (Review) PMID: 22884962
H171 residue in LIMP-2 is necessary for LIMP-2 and beta-glucocerebrosidase binding PMID: 22537104
Our data do not support the association of SNP rs6812193 with PD in Han Chinese of mainland China. PMID: 22465138
Two sisters are described with action myoclonus-renal failure (AMRF) syndrome resulting from a mutation in the SCARB2 gene. PMID: 21782476
This study supported a genetic contribution of the SCARB2 locus to PD; future studies in larger cohorts are necessary to verify this finding. PMID: 22223122
Coxsackievirus A infection is via celluar virus receptor SCARB2. PMID: 22438546
Results show that EV71 binds to SCARB2 and triggers a clathrin- and dynamin-dependent endocytosis for its entry. PMID: 22272359
Sequencing of SCARB2 genomic and cDNA identified a heterozygous, maternally inherited novel mutation (SCARB2 ) PMID: 21796727
The residues between 144 and 151 are critical for SCARB2 binding to VP1 of EV71 and seven residues from the human receptor could convert murine SCARB2, an otherwise inefficient receptor, to an efficient receptor for EV71 viral infection. PMID: 22219187
We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
in rare cases heterozygous SCARB2 mutations may be associated with demyelinating polyneuropathy features. PMID: 22032306
This study demonistrated that patients with SCARB2 mutations showed the clinical and neurophysiologic phenotype of progressive myoclonus epilepsy , extending the spectrum reported in the typical action myoclonus renal failure syndrome. PMID: 22050460
L929 cells expressing chimeras that carried amino acids 142 to 204 from the human sequence were susceptible to enterovirus 71, while chimeras that carried the mouse sequence in this region were not. PMID: 21389126
Action Myoclonus-Renal Failure Syndrome-causing mutations within LIMP-2 affect the binding to beta-glucocerebrosidase. PMID: 19933215
Several putative signalling motifs identified in the C-terminus of human SR-BII, which are absent from SR-BI, interact with signalling molecules to mobilize stored cholesteryl esters and/or promote the efflux of intracellular free cholesterol. PMID: 14570588
High density lipoprotein endocytosis by scavenger receptor SR-BII is clathrin-dependent and requires a carboxyl-terminal dileucine motif. PMID: 16368683
Residues favoring AP3 binding introduced into a protein that is transported via the PM such as the invariant chain can re-route such protein into direct sorting to late endosomal/lysosomal structures. PMID: 16542748
SCARB2/Limp2 has pleiotropic effects that may be relevant to understanding the pathogenesis of other forms of glomerulosclerosis or collapse and myoclonic epilepsies. PMID: 18308289
Scavenger receptor B2 is a cellular receptor for enterovirus 71. PMID: 19543282
Study of SCARB2 mutations finds unsolved cases of progressive myoclonus epilepsy without renal impairment, especially those resembling Unverricht-Lundborg disease. PMID: 19847901
Functional analysis of the mouse homolog PMID: 12620969

Hide All

Involvement in disease

Epilepsy, progressive myoclonic 4, with or without renal failure (EPM4)

Subcellular Location

Lysosome membrane; Multi-pass membrane protein.

Protein Families

CD36 family

Database Links

HGNC: 1665

OMIM: 254900

KEGG: hsa:950

STRING: 9606.ENSP00000264896

UniGene: Hs.349656

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA203929A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), A549 whole cell lysate(30µg), U251 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes:SCARB2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 54 kDa Observed band size: 77 kDa Exposure time：1min IHC image of CSB-RA203929A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA203929A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A431 cells stained with CSB-RA203929A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

SCARB2 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background