Product Details

Uniprot No.

Target Names

Alternative Names

CD329 antigen antibody; CDw329 antibody; SAF 2 antibody; SAF-2 antibody; SAF2 antibody; Sialic acid binding Ig like lectin 8 antibody; Sialic acid-binding Ig-like lectin 8 antibody; Sialoadhesin family member 2 antibody; SIGL8_HUMAN antibody; Siglec 8 antibody; Siglec-8 antibody; SIGLEC8 antibody; SIGLEC8L antibody

Species Reactivity

Human

Immunogen

Recombinant Human SIGLEC8 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

18D13

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SIGLEC8, also known as sialic acid-binding Ig-like lectin 8, serves as a critical inhibitory receptor predominantly expressed on eosinophils and mast cells. This cell surface glycoprotein plays a pivotal role in regulating allergic inflammation and immune responses by recognizing sialylated glycan ligands, making it an attractive target for researchers investigating asthma, allergic diseases, and eosinophilic disorders. The selective expression pattern of SIGLEC8 on these specific immune cell populations has also positioned it as a promising therapeutic target in immunology research.

This recombinant monoclonal antibody, clone 18D13, offers researchers the reproducibility and consistency that only sequence-defined antibodies can provide. Generated against recombinant human SIGLEC8 protein and produced with human IgG1 framework, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The affinity-chromatography purification ensures high purity suitable for demanding experimental workflows.

Validation in immunohistochemistry demonstrates reliable performance in formalin-fixed, paraffin-embedded human tissues. Testing in human prostate cancer tissue sections using citrate buffer antigen retrieval at pH 6.0 with dilutions of 1:100 yielded clear staining patterns when detected with HRP-conjugated secondary antibodies and DAB visualization. The antibody is also validated for ELISA applications, with recommended IHC dilutions ranging from 1:50 to 1:200 depending on tissue type and detection system sensitivity.

For investigators exploring signal transduction pathways in innate immunity or developing therapeutic strategies targeting eosinophil-mediated inflammation, this antibody provides a dependable tool for characterizing SIGLEC8 expression in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.

Gene References into Functions

In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family

Tissue Specificity

Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.

Database Links

HGNC: 10877

OMIM: 605639

KEGG: hsa:27181

STRING: 9606.ENSP00000321077

UniGene: Hs.447899

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Code	CSB-RA878931MA1HU
Size	US$210
	Order now
Image	IHC image of CSB-RA878931MA1HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
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SIGLEC8 Recombinant Monoclonal Antibody

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Target Background