Product Details

Uniprot No.

Target Names

Alternative Names

ASCT2 antibody; AAAT antibody; AAAT_HUMAN antibody; ATB(0) antibody; ATBO antibody; Baboon M7 virus receptor antibody; FLJ31068 antibody; M7V1 antibody; M7VS1 antibody; Neutral amino acid transporter B(0) antibody; R16 antibody; RD114/simian type D retrovirus receptor antibody; RDR antibody; RDRC antibody; SLC1A5 antibody; Sodium dependent neutral amino acid transporter type 2 antibody; Sodium-dependent neutral amino acid transporter type 2 antibody; Solute carrier family 1 (neutral amino acid transporter); member 5 antibody; Solute carrier family 1 member 5 antibody

Species Reactivity

Human

Immunogen

Recombinant Human SLC1A5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

4G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SLC1A5, also known as ASCT2, serves as a critical sodium-dependent neutral amino acid transporter that plays a central role in cellular glutamine uptake. This transporter has garnered significant research attention due to its marked upregulation in numerous cancer types, where it supports the elevated metabolic demands of rapidly proliferating tumor cells. Beyond oncology, SLC1A5 functions as a receptor for several retroviruses, including the baboon M7 virus and RD114, making it relevant to virology and gene therapy research.

This recombinant monoclonal antibody, clone 4G12, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and production occurs through controlled recombinant expression, you can expect consistent performance across different lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification further ensure high specificity for your target.

Validation studies demonstrate reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining has been successfully performed in both MCF and HeLa cells at 1:100 dilution, revealing clear membrane and cytoplasmic localization patterns consistent with the transporter's known cellular distribution. Flow cytometry analysis in A549 lung adenocarcinoma cells shows distinct positive population shifts compared to isotype controls, confirming the antibody's utility for quantitative single-cell analysis of SLC1A5 expression levels.

The antibody is validated for human samples and supplied in a glycerol-based buffer optimized for long-term storage stability. Whether investigating amino acid transport mechanisms, characterizing metabolic reprogramming in cancer models, or studying retroviral entry pathways, this antibody provides a dependable tool for your research workflow.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development.; (Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.; (Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.; (Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

Gene References into Functions

Data suggest that amino acid uptake via ASCT2/SLC1A5 is required for cell proliferation/tumor growth independently of LAT1/SLC7A5; in part, these studies were conducted in lung and colon adenocarcinoma cell lines and involved gene knockout techniques. (ASCT2/SLC1A5 = solute carrier family-1 member-5; LAT1/SLC7A5 = solute carrier family-7 member-5) PMID: 29326164
This study provides the metabolic molecule SLC1A5 as a potential therapeutic target to increase the efficacy of cetuximab on colorectal cancer PMID: 29363109
Results demonstrated that ASCT2 and pmTOR protein levels were significantly higher in epithelial ovarian cancer (EOC) tissues and predicting a poor prognosis. The expression levels of ASCT2 and pmTOR in EOC were positively correlated indicating a synergistic effect on the growth and development of early EOC. PMID: 30272366
intronic variants of the ASCT2 gene have roles in hampering the splicing process and in longevity PMID: 28885889
These data collectively establish that in an in vitro context, human epithelial and mesenchymal hepatocellular carcinoma cell lines adapt to ASCT2 or LAT1 knockout. PMID: 30029480
SLC1A5 expression is increased in tumor samples from esophageal cancer patients, and downregulation of SLC1A5 inhibited cell cycle progression and esophageal cancer growth. PMID: 30071532
Our results indicate that overexpression of SLC1a5 is associated with shorter overall survival in non-small cell lung cancer PMID: 29455869
the experimental data allowed identifying C467 residue as crucial for substrate binding and for transport activity modulation of hASCT2. PMID: 29495336
ASCT2 was significantly overexpressed in the gastric cancer (GC) samples compared with adjacent non-cancerous gastric mucosa, in contrast, a significantly higher level of glutamine synthetase (GS) expression was observed in normal tissues than in GC samples compared with adjacent non-cancerous gastric mucosa. PMID: 29435734
These results indicated that ASCT2 (SLC1A5) could be a novel therapeutic target against KRAS-mutant colorectal cancer. PMID: 28749408
AR signaling promoted glutamine metabolism by increasing the expression of the glutamine transporters SLC1A4 and SLC1A5, genes commonly overexpressed in prostate cancer. Correspondingly, gene expression signatures of AR activity correlated with SLC1A4 and SLC1A5 mRNA levels in clinical cohorts. PMID: 28507054
Results provide evidence that ASCT2 enhances glutamine uptake in glycolipid-enriched microdomain/rafts in GD2(+) small-cell lung cancer cells, leading to the enhancement of cell proliferation and migration. PMID: 29151270
High ASCT2 expression is associated with head and neck squamous cell carcinoma. PMID: 28823958
this study shows that IL-2-induced expression of SLC1A5 is a prerequisite for NKG2D-mediated activation of NK cells PMID: 28784848
Data suggest that both heterogeneous nuclear ribonucleoprotein type M (HNRPM) and solute carrier 1A5 (SLC1A5) have role in the pathogenesis of ovarian cancer. PMID: 28609484
Both stable and inducible shRNA-mediated ASCT2 knockdown confirmed that inhibiting ASCT2 function was sufficient to prevent cellular proliferation and induce rapid cell death in TN basal-like breast cancer cells, but not in luminal cells. PMID: 26455325
ASCT2 is an EGFR-associated protein that can be co-targeted by cetuximab, leading to sensitization of cetuximab-treated cells to ROS-induced apoptosis. PMID: 27450723
The tumor metabolism status determined by expression of GLUT1 and ASCT2. PMID: 28036362
Data show that SERT associates with ASCT2 (alanine-serine-cysteine-threonine 2), a member of the solute carrier 1 family co-expressed with SERT in serotonergic neurons and involved in the transport of small neutral amino acids across the plasma membrane. PMID: 27143784
In the absence of SLC1A5 there is a crucial role of SNAT1 in supplying glutamine for glutaminolysis with SNAT2 acting as a "backup" for glutamine transport. PMID: 27129276
High expression of LAT1 and ASCT2 correlates with metastasis and invasion in esophageal squamous cell carcinoma. PMID: 26936531
LAT1, 4F2hc and ASCT2 were highly expressed in patients with advanced laryngeal cancer. PMID: 26024742
Cys is a potent competitive inhibitor of hASCT2 but is not a substrate PMID: 26492990
Genetic Variants in ASCT2 Gene are Associated with response to therapy in Hepatocelluar Carcinoma PMID: 25987094
ASCT2 expression plays an important role in tumour cell growth, and is a promising pathological marker for predicting a worse outcome in pancreatic cancer. PMID: 24845232
The results demonstrate that N-glycosylation of SLC1A5 is critical for trafficking. PMID: 25862406
ASCT2-mediated glutamine uptake is essential for multiple pathways regulating the cell cycle and cell growth. PMID: 25693838
ASCT-2 is expressed in human oocytes.ASCT-2 is localized at the acrosomal region and equatorial segment of the spermatozoa. PMID: 24687878
Report the differential expression of SLC1A5 in human colorectal cancer and normal tissues, and a functional link between SLC1A5 expression and growth and survival of colorectal cancer. PMID: 25337245
analysis of the kinetic mechanism of the transport catalyzed by the human glutamine/neutral amino acid transporter hASCT2 PMID: 25052780
ASCT2 expression, which correlates with that of N-Myc and ATF4, is markedly elevated in high-stage neuroblastoma PMID: 25142020
the expression of LAT1 and ASCT2 is significantly increased in human melanoma. The importance of ASCT2 expression in melanoma was confirmed by shRNA knockdown, which inhibited glutamine uptake, mTORC1 signaling and cell proliferation. PMID: 24531984
ASC amino-acid transporter 2 expression has a crucial role in the metastasis of pulmonary AC, and is a potential molecular marker for predicting poor prognosis after surgery. PMID: 24603303
HER2- type breast cancer had the highest expression of stromal GLS1, tumoral GDH, stromal GDH, and tumoral ASCT, while TNBC had the lowest tumoral GDH expression. PMID: 23507704
stromal expression of the glutamine-metabolism-related proteins GLS1, GDH, ASCT2 increases with worsening histological phyllodes tumor grade. PMID: 23636801
SLC1A5 plays a key role in Gln transport controlling lung cancer cells' metabolism, growth, and survival. PMID: 23213057
These data suggest that intracellular replication of Francisella tularensis depends on the function of host cell SLC1A5. PMID: 22804921
Cx43-mediated GJIC and SLC1A5 interaction play important functional roles in trophoblast cell fusion. PMID: 22238282
level of amino acid transporter B(0)(ACST2) mRNA expression was lower in forskolin treated BeWo cells PMID: 12175968
in the six unrelated Hartnup pedigrees studied, examination of linkage at 19q13.3, polymorphisms in the coding sequence and quantitation of expression of SLC1A5 did not suffice to explain the defect in neutral amino acid transport PMID: 12555937
Hypoxia alters expression and function of syncytin and its receptor (amino acid transport system B(0)) during trophoblast cell fusion of human placental BeWo cells: implications for impaired trophoblast syncytialisation in pre-eclampsia. PMID: 12757936
ASCT2 messenger RNA was not altered significantly in hypoxia in cytotrophoblasts or in perfused placental cotyledons. PMID: 14520239
up-regulation of ASCT2 by S-nitroso-N-acetyl-DL-penicillamine might be partially associated with an increase in the density of transporter protein via de novo synthesis PMID: 15848195
analysis of SLC1A5 mutations on 19q13 in cystinuria patients PMID: 16435221
evaluation of the interaction of the HERV-W envelope with the hASCT2 receptor; a region consisting of the N-terminal 124 amino acids of the mature glycoprotein surface subunit was determined as the minimal receptor-binding domain PMID: 16820059
ASCT2 silencing inhibits mTORC1 (mTOR/raptor) signaling and leads to growth repression, followed by enhanced survival signaling via mTORC2 (mTOR/rictor comples) and apoptosis of hepatoma cells. PMID: 17329400
Localisation and distribution of RDR/ASCT2 in human placental villi suggests that the fusion of placental trophoblast cells is not regulated by local or temporal variations of RDR/ASCT2 expression in villous cytotrophoblast cells. PMID: 17676482
In human entrocytes, glutamine transport and ASCT2 surface expression induced by short-term EGF are MAPK, PI3K, and Rho-dependent. PMID: 18157695
SLC1A5 gene is associated with schizophrenia. PMID: 18638388
In primary term human trophoblasts, ASCT2 mRNA levels were preserved at normoxia and downregulated at 1% O(2) after 48 h. PMID: 19321927

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A5 subfamily

Tissue Specificity

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Database Links

HGNC: 10943

OMIM: 109190

KEGG: hsa:6510

STRING: 9606.ENSP00000444408

UniGene: Hs.631582

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Code	CSB-RA620892MA1HU
Size	US$210
	Order now
Image	Immunofluorescence staining of MCF cell with CSB-RA620892MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Immunofluorescence staining of Hela cell with CSB-RA620892MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Overlay Peak curve showing A549 cells stained with CSB-RA620892MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SLC1A5 Recombinant Monoclonal Antibody

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