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| Code | CSB-RA912385A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
CUSABIO meticulously produced the SLC25A12 recombinant monoclonal antibody following a well-defined process. Initially, B cells were extracted from the spleen of an immunized animal, using a synthesized peptide derived from human SLC25A12 as the immunogen. Subsequently, isolating RNA from the B cells and converting the extracted RNA into cDNA. The gene encoding the SLC25A12 antibody was amplified using a degenerate primer and inserted into a vector. The recombinant vector containing the SLC25A12 antibody gene was then introduced into host cells through transfection, allowing for the production of the SLC25A12 recombinant monoclonal antibodies. These antibodies were harvested from the cell culture supernatant and purified using affinity chromatography. Thorough validation, including ELISA and WB testings, was carried out to ensure the SLC25A12 recombinant monoclonal antibody's specific reactivity with human SLC25A12 protein, validating its reliability and suitability for a wide range of applications.
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