SMARCC1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA252590A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in: Jurkat whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), colo205 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: SMARCC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 123 kDa Observed band size: 155 kDa Exposure time：120s IHC image of CSB-RA252590A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA252590A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA252590A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q92922

Target Names

SMARCC1

Alternative Names

AI115498 antibody; BAF 155 antibody; BAF155 antibody; BRG 1 associated factor 155 antibody; BRG1 associated factor 155 antibody; BRG1-associated factor 155 antibody; Chromatin remodeling complex BAF155 subunit antibody; CRACC 1 antibody; CRACC1 antibody; Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody; Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody; Rsc 8 antibody; Rsc8 antibody; SMARC C1 antibody; SMARCC 1 antibody; SMARCC1 antibody; SMRC1_HUMAN antibody; SRG 3 antibody; SRG3 antibody; SWI 3 antibody; SWI/SNF complex 155 kDa subunit antibody; SWI/SNF complex subunit SMARCC1 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody; SWI3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SMARCC1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13D1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SMARCC1, also known as BAF155, serves as a core subunit of the SWI/SNF chromatin remodeling complex, playing an essential role in regulating gene expression through nucleosome repositioning. This protein is critical for numerous cellular processes including differentiation, proliferation, and DNA repair, making it a key target for researchers investigating epigenetic regulation and nuclear signaling pathways. Alterations in SWI/SNF complex components have been implicated in various cancers and developmental disorders, underscoring the importance of reliable detection tools for studying SMARCC1 biology.

This recombinant monoclonal antibody, generated against a synthesized peptide from human SMARCC1, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies.

Validation across multiple applications demonstrates this antibody's versatility in your research toolkit. Western blot analysis detects SMARCC1 in diverse human cell lines including Jurkat, SH-SY5Y, Colo205, and A431, with the observed band at 155 kDa appearing larger than the predicted 123 kDa molecular weight—a difference attributable to post-translational modifications such as glycosylation or phosphorylation commonly observed with chromatin-associated proteins. Immunohistochemistry staining has been validated in human testis tissue, while immunofluorescence in HeLa cells confirms nuclear localization consistent with SMARCC1's chromatin remodeling function. Flow cytometry analysis in SH-SY5Y cells further expands experimental options for quantitative single-cell studies.

Whether investigating chromatin dynamics, epigenetic mechanisms, or SWI/SNF complex function, this antibody provides a dependable foundation for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Gene References into Functions

Study found SMARCC1 as a direct target of miR-202-5p and promoted the growth and metastasis of colorectal carcinoma (CRC) cells. Furthermore, SMARCC1 could reverse the inhibitory effect of miR-202-5p on growth and metastasis of CRC cells. PMID: 30144500
our data showed that Swi3 strongly affects haem/oxygen-dependent activation of respiration gene promoters whereas Swi2 affects only the basal, haem-independent activities of these promoters. using computational analysis and RNAi knockdown, we showed that the mammalian Swi3 BAF155 and BAF170 regulate respiration in HeLa cells. PMID: 27190130
Results show the secondary structure of SWIRM domain of BAF155 consists of five alpha-helices forming a typical histone fold for DNA interactions. PMID: 23996527
we identify BAF155 as a substrate for arginine methyltransferase CARM1. PMID: 24434208
Wwp2 acts as a ubiquitin ligase of SRG3. PMID: 24365151
miR-320c regulates the resistance of pancreatic cancer cells to gemcitabine through SMARCC1. PMID: 23799850
loss of BAF155 expression represents another mechanism for inactivation of SWI/SNF complex activity in the development in human cancer. PMID: 22139574
Data show that the mechanism of BAF155-mediated stabilization of BAF57 involves blocking its ubiquitination by preventing interaction with TRIP12. PMID: 20829358
protein levels of BAF155/170 dictate the maximum cellular amount of BAF57 PMID: 16199878
Constitutive expression of SRG3 inhibits positive selection processes in T-cell receptor transgenic mice. PMID: 17513758
An increased expression of SMARCC1 protein was found in prostate cancer positively correlated with tumour dedifferentiation, progression, metastasis and time to recurrence. PMID: 18581278
patients with tumours displaying high levels of CBFB and SMARCC1 proteins had a significantly better overall survival rate than patients with low levels PMID: 19156145

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

SMARCC family

Tissue Specificity

Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.

Database Links

HGNC: 11104

OMIM: 601732

KEGG: hsa:6599

STRING: 9606.ENSP00000254480

UniGene: Hs.476179

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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