SNX5 Recombinant Monoclonal Antibody

Sorting nexin 5 (SNX5) plays a central role in intracellular trafficking and endosomal sorting, functioning as a key component of the retromer complex that mediates cargo retrieval from endosomes to the trans-Golgi network. This protein's involvement in membrane remodeling and receptor recycling makes it an important target for researchers investigating signal transduction pathways, autophagy, and cellular homeostasis mechanisms.

This recombinant monoclonal antibody against SNX5 offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology using a synthesized peptide derived from human SNX5, this rabbit monoclonal (clone 5E3) provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. Researchers can confidently build on previous results knowing each vial delivers identical performance characteristics.

Validation studies demonstrate reliable detection across multiple experimental platforms. In Western blot applications, the antibody detects a band at the predicted molecular weight of 47 kDa in human cell lines including A431, A549, and MCF-7, as well as in rat brain tissue lysate, confirming cross-species reactivity between human and rat samples. The antibody performs effectively at dilutions ranging from 1:500 to 1:5000 for Western blotting, offering flexibility to optimize signal intensity for different sample types. Immunohistochemistry validation in paraffin-embedded human kidney tissue and lung cancer specimens at 1:100 dilution demonstrates suitability for tissue-based studies using standard citrate buffer antigen retrieval protocols.

Whether investigating endosomal trafficking mechanisms, characterizing retromer function, or exploring SNX5's emerging roles in disease contexts, this affinity-purified antibody provides a dependable tool for signal transduction research across multiple validated applications.