Product Details

Uniprot No.

Target Names

Alternative Names

Sex determining region Y box 1 antibody; sox1 antibody; SOX1_HUMAN antibody; SRY antibody; SRY box containing gene 1 antibody; SRY related HMG box gene 1 antibody; Transcription factor Sox-1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SOX1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SOX1 is a transcription factor belonging to the SRY-related HMG-box family, playing a critical role in neural development and the maintenance of neural progenitor cell identity. As an early marker of neuroectodermal differentiation, SOX1 has become increasingly valuable in stem cell research, developmental biology, and cancer studies where aberrant expression patterns may influence tumor progression and cellular differentiation states.

This recombinant monoclonal antibody, clone 7B7, offers researchers the reproducibility and consistency that recombinant technology provides. Because the antibody sequence is defined and produced from a stable expression system, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, combined with affinity chromatography purification, ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing on paraffin-embedded human lung cancer tissue using a Leica Bond system with citrate buffer antigen retrieval shows clear detection at 1:100 dilution, making this antibody well-suited for investigating SOX1 expression in archival clinical specimens. Flow cytometry analysis using A549 cells confirms the antibody's utility for single-cell analysis, with clear separation from isotype control when used at 1:100 dilution on fixed and permeabilized cells.

The unconjugated format provides flexibility for pairing with your preferred secondary detection systems, whether for chromogenic IHC visualization or fluorescent flow cytometry applications. For researchers investigating epigenetics, nuclear signaling, neural differentiation, or cancer biology, this antibody delivers the specificity and batch consistency needed for meaningful, reproducible results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA449123A0HU
Size	US$210
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Image	IHC image of CSB-RA449123A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing A549 cells stained with CSB-RA449123A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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SOX1 Recombinant Monoclonal Antibody

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