Code | CSB-RA547973A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
IF | 1:50-1:200 |
The development of the STAT4 recombinant monoclonal antibody involves a rigorous and standardized process to ensure its quality and specificity. B cells are initially isolated from an immunized animal, with the synthesized peptide derived from human STAT4 used as the immunogen. Total RNA is extracted from the B cells and converted into cDNA through reverse transcription. The STAT4 antibody genes are amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. The vector is then introduced into host cells, enabling the production of the STAT4 recombinant monoclonal antibody. After harvesting from the cell culture supernatant, the antibody is purified using affinity chromatography to obtain a highly purified form. Extensive characterization assays, including ELISA, IHC, and FC analysis, are performed to validate the antibody's specificity and functionality, ensuring its accurate binding to human STAT4 protein. This meticulous production process guarantees the generation of a reliable and effective STAT4 recombinant monoclonal antibody, suitable for various applications in STAT4-related research.
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