STX16 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA027547A0HU
Size	US$210
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Image	IHC image of CSB-RA027547A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

O14662

Target Names

STX16

Alternative Names

hsyn16 antibody; MGC90328 antibody; Stx16 antibody; STX16_HUMAN antibody; Syn16 antibody; Syntaxin-16 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human STX16 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Syntaxin-16 plays a central role in intracellular membrane trafficking, functioning as a SNARE protein that mediates vesicle fusion events within the Golgi apparatus and endosomal compartments. This trafficking machinery is essential for maintaining cellular homeostasis, protein sorting, and secretory pathway function, making STX16 a valuable marker for researchers investigating membrane dynamics, organelle identity, and vesicular transport mechanisms.

This recombinant monoclonal antibody, clone 17B11, offers the reproducibility and consistency that demanding research protocols require. Generated against a synthetic peptide derived from human STX16 protein, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides robust signal amplification compatible with standard secondary detection systems.

Validation in immunohistochemistry demonstrates reliable performance in paraffin-embedded human tissues. Testing in human testis sections using a Leica Bond automated staining platform with citrate buffer antigen retrieval and HRP-polymer detection confirms the antibody's suitability for formalin-fixed samples at dilutions ranging from 1:50 to 1:200. This flexibility allows researchers to optimize signal intensity based on their specific tissue types and detection systems. The antibody is additionally validated for ELISA applications, providing options for both quantitative and spatial analysis of STX16 expression.

Supplied in a glycerol-containing buffer optimized for long-term storage stability, this affinity-purified antibody serves researchers exploring Golgi organization, membrane trafficking pathways, and cellular compartmentalization. Its defined specificity for human STX16 makes it particularly suited for studies examining vesicular transport in normal physiology and disease contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.

Gene References into Functions

we here present a patient with PHP1b caused by a recurrent STX16 deletion, presenting with macrosomia, early onset obesity, and macrocephaly without other AHO symptoms. we reemphasize STX16 deletions and PHP1b as a rare cause for early onset obesity and macrosomia. PMID: 27338644
STX16 microdeletion was identified in male monozygotic twins (with pseudohypoparathyroidism type 1B leading to growth hormone deficiency) and mother/grandmother (not father/grandfather or sister [their triplet with separate placenta]). [CASE STUDY] PMID: 25843330
syntaxin 16 is a key regulator of cytokinesis. PMID: 24109596
A patient with familial pseudohypoparathyroidism type Ib and his asymptomatic brother were found to have methylation defect at GNAS (guanine nucleotide-binding protein G) and microdeletion involving exons 4-6 of neighboring gene STX16. [CASE REPORT] PMID: 23095209
De novo 3-kb STX16 deletions, reported only once previously, are infrequent but should be excluded in all cases of Pseudohypoparathyroidism-Ib, even when the family history is negative for an inherited form of this disorder. PMID: 23087324
Results suggest that STX16 mediates recycling of CFTR and constitutes an important component of CFTR trafficking machinery in intestinal epithelial cells. PMID: 20826815
the region of overlap between the two microdeletions likely harbors a cis-acting imprinting control element that is necessary for establishing and methylation at GNAS exon A/B, thus allowing normal G alpha(s) expression in the proximal renal tubules. I PMID: 15800843
function of syntaxin 16 was specifically required for, and restricted to, the retrograde pathway PMID: 17389686
Syntaxin 16 may thus play a role in neurite outgrowth and perhaps other specific dendritic anterograde/retrograde traffic. PMID: 17852734
phosphorylation of RASSF1A by Aurora B is required for the recruitment of Syntaxin16 PMID: 19887622

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Involvement in disease

Pseudohypoparathyroidism 1B (PHP1B)

Subcellular Location

Golgi apparatus membrane; Single-pass type IV membrane protein.; [Isoform C]: Cytoplasm.

Protein Families

Syntaxin family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 11431

OMIM: 603233

KEGG: hsa:8675

STRING: 9606.ENSP00000360183

UniGene: Hs.307913

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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