Product Details

Uniprot No.

Target Names

TARBP2

Alternative Names

LOQS antibody; Prbp antibody; RISC loading complex subunit TARBP2 antibody; RISC-loading complex subunit tarbp2 antibody; TAR (HIV 1) RNA binding protein 2 antibody; TAR (HIV) RNA binding protein 2 antibody; TAR (HIV) RNA-binding protein TRBP1 antibody; TAR RNA Binding Protein 2 antibody; TAR RNA-binding protein 2 antibody; tarbp2 antibody; TARBP2 RISC loading complex RNA binding subunit antibody; Trans-activation-responsive RNA-binding protein antibody; TRBP antibody; TRBP1 antibody; TRBP2 antibody; TRBP2_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human TARBP2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TARBP2 (TAR RNA-binding protein 2) serves as an essential component of the RNA-induced silencing complex (RISC), where it partners with Dicer to facilitate microRNA processing and loading onto Argonaute proteins. This central role in the miRNA pathway makes TARBP2 a compelling target for researchers investigating post-transcriptional gene regulation, viral RNA interactions, and the dysregulation of small RNA networks in disease contexts. Originally identified through its ability to bind HIV-1 TAR RNA elements, TARBP2 continues to attract attention in both virology and cancer biology research.

This recombinant monoclonal antibody, clone 8G12, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human TARBP2 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Affinity chromatography purification ensures high purity for reliable performance across applications.

Validation studies demonstrate robust performance in immunohistochemistry and flow cytometry applications. IHC analysis of paraffin-embedded human glioma tissue using citrate buffer antigen retrieval shows clear target detection at 1:100 dilution, making this antibody suitable for investigating TARBP2 expression in tumor specimens. Flow cytometry validation in HepG2 hepatocellular carcinoma cells confirms effective intracellular staining of fixed and permeabilized cells, with clear separation from isotype control, enabling quantitative analysis of TARBP2 expression at the single-cell level.

This antibody supports investigations into miRNA biogenesis mechanisms, RISC assembly dynamics, and the emerging connections between TARBP2 dysregulation and oncogenic processes across multiple cancer types.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.; (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system.

Gene References into Functions

These results establish that PKR regulation through stress-induced TRBP phosphorylation is an important mechanism ensuring cellular recovery and preventing apoptosis due to sustained PKR activation. PMID: 29348664
A statistically significant association with the risk of AD was observed with the CT genotype for rs784567 on the TARBP2 gene. on controlling for age and sex, we found that for the TARBP2-RNASEN association with AD the age variation was a risk factor for AD risk (P<0.001; OR=1.104; 95% CI, 1.059-1.151). PMID: 26796812
TRBP is a novel transcriptional coactivator of the Notch signaling pathway, playing an important role in neural stem cell regulation during mammalian brain development PMID: 28174252
TRBP is required for normal antiviral responses to Cardiovirus infection. PMID: 27743889
Experimental and computational techniques were employed to examine the hypothesis that TRBP-double-stranded RNA-binding domain (dsRBD) 2 binding by a 20-mer dsRNA is coupled with RNA bending, results suggest that this particular dsRBD-dsRNA interaction produces little to no change in the A-form geometry of dsRNA in solution PMID: 27332119
We propose that the S6K2/TRBP node controls miRNA biogenesis in HDLECs and provides a molecular link between the mTOR pathway and the miRNA biogenesis machinery. PMID: 27407113
TARBP2 silencing inhibits in vitro invasion and migration of NCIH1299 nonsmall cell lung cancer cell via the JNK/STAT3/AKT pathway. TARBP2 silencing does not significantly affect cell growth. PMID: 27599909
TARBP2 has roles in tumor development and progression [review] PMID: 26486325
SUMOylation of TARBP2 is required for regulating siRNA efficiency. PMID: 26582366
HIV-1 Rev-response element RNA acts as an RNA silencing suppressor by competing with TRBP-bound siRNAs. PMID: 25668122
the role of TRBP and unveils negative feedback regulation of PKR through TRBP phosphorylation. PMID: 25437560
Results show the crystal structure of the interface between microRNA biogenesis proteins Dicer and TRBP. Mutations in this interface prevent recruitment of TRBP to Dicer. PMID: 25557550
RPL5 binds to the TRBP2 and Ago2 subunits of the RISC and mediates the binding to c-Myc 3'UTR. PMID: 24141778
TARBP2 expression is upregulated in late-stage breast cancer and triple-negative breast cancer and may have a role in poorer disease-free survival and overall survival PMID: 24563327
identified TARBP2, a double-stranded RNA-binding protein implicated in microRNA processing, as the trans factor that binds the sRSE family and similar structural elements--collectively termed TARBP2-binding structural elements (TBSEs)--in transcripts PMID: 25043050
Tarbp2 binding to TRPC4 promotes changes of cytosolic Ca(2+) and, thereby, leads to a dynamic regulation of Dicer activity, essentially at low endogenous Dicer concentrations. PMID: 24563462
mRNA expression of TARBP2, but not DICER or DROSHA, is a strong molecular predictor to discriminate between adrenocortical adenomas and carcinomas PMID: 23671264
in vitro binding patterns of human TRBP and PACT to siRNA PMID: 23658827
The results show that PACT and TRBP have distinct effects on Dicer-mediated dsRNA processing. PMID: 23661684
No frameshift mutations in TARBP2 were identified in a study of 4 cases of upper urinary tract urothelial carcinoma with high microsatellite instability status. PMID: 23690119
Results show that the region of TRBP that binds immature miRNAs comprises two independent double-stranded RNA-binding domains connected by a 60-residue flexible linker. PMID: 23435228
RISC proteins PACT, TRBP, and Dicer, together with PKR, are steroid receptor RNA activator-binding nuclear receptor coregulators that are recruited to the promoters of hormone-regulated genes and regulate the expression of downstream target genes. PMID: 23550157
We report an unanticipated dsRNA diffusion behavior of TRBP, which requires two double-stranded RNA binding domains, and its correlation with the role of TRBP in promoting Dicer-induced RNA cleavage. PMID: 23251028
The studies in this review show that TRBP is multifunctional and mediates cross talk between different pathways. Its activities at the molecular level impact the cellular function from normal development to cancer and the response to infections. PMID: 22933564
There was no significant difference in the TARBP2 expression levels between the epithelial skin cancer groups and the healthy controls (P > 0.05). PMID: 22025453
Depletion of Dicer and TRBP, proteins involved in miRNA biogenesis, reduced HCV RNA accumulation, mature duplex miR-122 abundance, and miR-122 directed mRNA translation suppression, suggesting roles in miR-122 processing. PMID: 22999255
TRBP is a key regulator of miRNA processing and targeting in humans. PMID: 23006623
Data suggest that cancer stem cell (CSC) self-renewal and tumor maintenance may depend on deregulation of TARBP2-dependent miRNA expression. PMID: 22698405
AGO2, PACT and TRBP are required for the efficient functioning of Dicer in cells PMID: 22163034
results demonstrate for the first time that stress-induced PACT phosphorylation functions to free PACT from the inhibitory interaction with TRBP and also to enhance its interaction with PKR PMID: 21526770
The structures dsRNA-bdinding domain 1 and dsRNA binding domain 2 solved by x-ray crystallography and NMR spectroscopy respectively. PMID: 21080422
TARBP2 frameshift mutation is associated with hereditary nonpolyposis colorectal cancer. PMID: 20877318
the three-dimensional structure of human Dicer bound to the protein TRBP at approximately 20 A resolution determined by negative-stain electron microscopy (EM) and single-particle analysis PMID: 19836333
binds to merlin, which inhibits its oncogenic activity; results provide the first clue to the functional interaction between TRBP and merlin and suggest a novel mechanism for the tumor suppressor function of merlin both in vitro and in vivo PMID: 15123692
Data suggest that the cell specifity of HIV-1 expression and replication may be regulated, in part, through the control of TRBP1 expression by NF-Y factors. PMID: 16343534
These results suggest that, in the context of HIV replication, TRBP contributes mainly to the enhancement of virus production and that Dicer does not mediate HIV restriction by RNAi. PMID: 17360756
Results indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. PMID: 17452327
Ago2, Dicer, and TRBP comprise the RISC-loading complex (RLC) and assembles spontaneously in vitro from purified components PMID: 18178619
TRBP controls PACT activation of PKR, an activity that is reversed by stress. PMID: 18936160
RDE-4 preferentially binds long dsRNA, while TRBP binds siRNA with an affinity that is independent of dsRNA length. PMID: 18948111
The RLC Dicer's N-terminal DExH/D domain, located in a short 'base branch', interacts with TRBP, whereas its C-terminal catalytic domains in the main body are proximal to AGO2. PMID: 19820710

Hide All

Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Nucleus.

Protein Families

TARBP2 family

Database Links

HGNC: 11569

OMIM: 605053

KEGG: hsa:6895

STRING: 9606.ENSP00000266987

UniGene: Hs.326

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA778009A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA778009A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA778009A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

TARBP2 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background