Code | CSB-RA696677A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The production of the recombinant TOP2A antibody depended on Single B Cell technology. There are 3 main steps in the production: 1, Isolation of single B cells. High-throughput methods could be used to obtain the efficient identification and desired specificity of B cells. 2, Single B cell antibody sequencing and cloning. In this step, the antibody gene sequence of TOP2A was obtained and introduced to plasmids, which then would be transferred to mammalian cells for in vitro expression of the TOP2A antibody. 3, Screening of antibodies. The target antibody was obtained in this step. And it has been validated in ELISA, WB, IHC.
Topoisomerase II isozyme TOP2A is a key component of mitotic chromosomes and is required for mitotic chromosome condensation in mammalian cells. TOP2A is involved in chromosomal segregation and DNA replication. It functions specifically chromosomal untangling and is essential for sister chromatid segregation prior to anaphase. It's also needed to activate the decatenation checkpoint. In anaphase, TOP2A is essential for centromere decatenation and the resolution of chromatin bridges and ultrafine DNA bridges (UFBs).
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