TPSB2 Recombinant Monoclonal Antibody

Tryptase beta-2, encoded by the TPSB2 gene, is a serine protease predominantly expressed in mast cells where it serves as a major secretory granule component. This enzyme plays significant roles in inflammatory responses, allergic reactions, and tissue remodeling, making it a valuable marker for mast cell activation and a target of interest in immunology, allergy research, and inflammatory disease studies.

This recombinant monoclonal antibody against TPSB2 offers the consistency and reliability that demanding research applications require. Developed using recombinant technology with clone 9H6, it provides sequence-defined specificity and eliminates the lot-to-lot variability that can compromise experimental reproducibility. The antibody is raised in rabbit against a synthetic peptide derived from human TPSB2, delivering precise epitope targeting with cross-species reactivity to both human and mouse samples.

Validation studies demonstrate robust performance across multiple platforms. Western blot analysis of mouse kidney tissue lysate reveals clean detection at the predicted molecular weight of 30 kDa, with the observed band size matching expectations precisely—indicating reliable target recognition without significant post-translational modification interference in this tissue context. Flow cytometry validation using K562 cells confirms intracellular detection capability, with clear signal separation from isotype control when cells are properly fixed and permeabilized.

The antibody's versatility extends across ELISA, Western blot, and flow cytometry applications, with optimized working dilutions established for each platform. Supplied in a glycerol-containing buffer for stability, this affinity-purified reagent supports investigations into mast cell biology, allergic inflammation, asthma pathophysiology, and conditions where tryptase activity contributes to disease mechanisms.