Human Tryptase beta-2(TPSB2) ELISA kit

Human Tryptase beta-2(TPSB2) ELISA kit

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Human Tryptase beta-2(TPSB2) ELISA kit

Protocol MSDS

Code	CSB-EL024128HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Alternative Names

Mast cell tryptase beta II ELISA Kit; Mast cell tryptase beta III ELISA Kit; TPS2 ELISA Kit; TPSB2 ELISA Kit; TRYB2_HUMAN ELISA Kit; Tryptase beta 2 (gene/pseudogene) ELISA Kit; Tryptase beta-2 ELISA Kit; Tryptase II ELISA Kit; Tryptase-2 ELISA Kit; TryptaseB ELISA Kit; TryptaseC ELISA Kit

Abbreviation

TPSB2

Uniprot No.

P20231

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.137 ng/ml - 100 ng/ml

Sensitivity

0.137 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	89
	Range %	84-95
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	83-97

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

100

2.576

2.634

2.605

2.502

33.33

1.807

1.767

1.787

1.684

11.11

1.124

1.188

1.156

1.053

3.7

0.639

0.629

0.634

0.531

1.23

0.388

0.371

0.380

0.277

0.41

0.296

0.286

0.291

0.188

0.137

0.185

0.184

0.185

0.082

0.101

0.105

0.103

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Function
(From Uniprot)

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.

Gene References into Functions

These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655

Subcellular Location

Secreted. Note=Released from the secretory granules upon mast cell activation.

Protein Families

Peptidase S1 family, Tryptase subfamily

Database Links

HGNC: 14120

OMIM: 191081

KEGG: hsa:64499

UniGene: Hs.405479

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.