Product Details

Uniprot No.

P13693

Target Names

TPT1

Alternative Names

Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23), TPT1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human TCTP

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Translationally-controlled tumor protein, encoded by the TPT1 gene, serves as a multifunctional regulator involved in cell growth, survival, and immune modulation. Also known as fortilin or histamine-releasing factor, this protein plays significant roles in apoptosis inhibition, calcium binding, and cytokine-like activities that influence allergic responses and inflammation. Its elevated expression in various proliferative conditions makes TPT1 a compelling target for researchers investigating cell cycle regulation, stress responses, and immunological pathways.

This recombinant monoclonal antibody, generated in rabbit using clone 10A9, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The antibody has been raised against a synthetic peptide derived from human TCTP and purified through affinity chromatography to maximize specificity.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis confirms detection in an extensive panel of human cell lines including HeLa, Jurkat, MCF-7, HepG2, PC-3, Raji, and 293 cells, as well as RAW264.7 mouse cells and mouse liver tissue, supporting cross-species reactivity for both human and mouse samples. The observed band at approximately 25 kDa runs slightly higher than the predicted molecular weight, likely reflecting post-translational modifications such as glycosylation. Immunohistochemistry validation in human prostate tissue and immunofluorescence staining in SY5Y neuroblastoma cells demonstrate versatility for tissue and cellular localization studies. Successful immunoprecipitation from HeLa lysates further confirms utility for protein interaction investigations.

This antibody provides immunology researchers with a reliable tool for exploring TPT1 function across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA214107A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue All lanes: TPT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 16 kDa Observed band size: 25 kDa IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating TCTP in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA214107A0HU(2µg)+ Hela whole cell lysate(500µg) Lane 3: Hela whole cell lysate (10µg)
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TPT1 Recombinant Monoclonal Antibody

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