TXN2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA053471A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), K562 whole cell lysate(30µg), A549 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), LO2 whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Rat liver tissue lysate(30µg) All lanes: Thioredoxin 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 18 kDa Observed band size: 12 kDa Exposure time：2min IHC image of CSB-RA053471A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA053471A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA053471A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA053471A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q99757

Target Names

TXN2

Alternative Names

Thioredoxin, mitochondrial (MTRX) (Mt-Trx) (Thioredoxin-2), TXN2, TRX2

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Thioredoxin 2 (TXN2) serves as a critical mitochondrial redox regulator, maintaining the reduced state of proteins within the mitochondrial matrix and protecting cells against oxidative stress. This mitochondria-specific thioredoxin plays essential roles in cellular defense mechanisms, apoptosis regulation, and mitochondrial function, making it a significant target for researchers investigating oxidative stress responses, mitochondrial biology, and various disease states where redox imbalance contributes to pathology.

This recombinant monoclonal antibody, developed using rabbit host immunization against a synthetic peptide derived from human TXN2, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined recombinant clone (10C11), it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your experimental conditions remain constant across projects.

Validation studies demonstrate robust performance across multiple platforms. Western blot analysis confirms detection in a diverse panel of human cell lines including HEK293, Jurkat, Raji, K562, A549, HepG2, and LO2, with cross-species reactivity extending to mouse and rat liver tissue lysates. The observed band at approximately 12 kDa, smaller than the 18 kDa predicted molecular weight, likely reflects post-translational processing or cleavage of the mitochondrial targeting sequence upon import. Immunohistochemistry validation in human liver and liver cancer tissue sections shows clear staining patterns, while immunofluorescence studies in HeLa cells and flow cytometry analysis of Jurkat cells further confirm the antibody's versatility for cellular localization and quantitative studies.

This antibody supports investigations into mitochondrial dysfunction, cancer metabolism, neurodegenerative diseases, and cellular stress responses where understanding TXN2 expression and localization provides mechanistic insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Important for the control of mitochondrial reactive oxygen species homeostasis, apoptosis regulation and cell viability. Possesses a dithiol-reducing activity.

Gene References into Functions

Overexpression of mitochondrial Trx2 attenuates the intracellular ROS accumulation and ATP production. Most interestingly, mitochondrial Trx2 may be involved in the cardiac hypertrophy signaling of diabetes. PMID: 28914755
Trx2 overexpression failed to attenuate hypoxia-induced human pulmonary arterial smooth muscle cells proliferation in vitro or hypoxia-induced Pulmonary hypertension in vivo. PMID: 28130258
study demonstrated that miR-27a and -b, which are widely expressed in host cells, suppress SNAP25 and TXN2 expression through posttranscriptional gene silencing. PMID: 28356525
No evidence that SNPs in TRX2 have effects, but the rs4485648 polymorphism of the TrxR2 gene might exert an independent effect on the development of Diabetic retinopathy. PMID: 26763822
Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus by promoting the nuclear translocation of the p65 subunit of NF-kappaB. PMID: 26041303
The Grx2 system could help to keep Trx2/1 reduced during an oxidative stress, thereby contributing to the anti-apoptotic signaling. PMID: 24295294
CERKL interacts with TRX2 and plays a novel key role in the regulation of the TRX2 antioxidant pathway. PMID: 24735978
TGF-beta-mediated expression of the epithelial-mesenchymal transition marker fibronectin was inhibited not only by chemicals that interfere with reactive oxygen species signaling but also by exogenously expressed mitochondrial thioredoxin. PMID: 24342608
We showed that overexpression of TRXs reduced cell death; TRX2 was expressed in the mitochondria, while TRX1 was expressed in the cytoplasm. PMID: 23485938
knockdown of TRX-1 or TRX-2 sensitizes cells to CYP2E1-induced oxidant stress partially via ASK-1 and JNK1 signaling pathways. Both TRX-1 and TRX-2 are important for defense against CYP2E1-induced oxidative stress. PMID: 21557999
Data show that knockdown of S100P led to downregulation of thioredoxin 1 and beta-tubulin and upregulation of RhoGDIA, all potential therapeutic targets in cancer. PMID: 21327297
TRX1 and TRX2 regulate the proliferation and survival of adipocyte derived stem cells; these processes are mediated by the activation of ERK1/2 PMID: 21158569
mitochondrial thioredoxin may play important roles in protection against oxidant-induced apoptosis PMID: 12032145
MT-TRX has a role in the regulation of the mitochondrial membrane potential and cell death PMID: 12080052
Thioredoxin 1 and thioredoxin 2 have opposed regulatory functions on hypoxia-inducible factor-1alpha. PMID: 17220299
The LC/ICPMS analyses showed that the trivalent arsenic species were able to form complexes with both human and E. coli Trx. PMID: 17939155
upon stimulation of Fas, thioredoxin-2 mediated denitrosylation of mitochondria-associated caspase-3, a process required for caspase-3 activation, and promoted apoptosis PMID: 18497292
a novel Ins/Del polymorphism in the human TXN2 gene proximal promoter region that altered the transcriptional activity and is associated with spina bifida risk PMID: 19165900
Trx2 modulates transcription of GR and NF-kappaB reporter genes. PMID: 19570036
These results suggest that TRX2 not only functions as an antioxidant, but also supports mtTFA functions PMID: 19885567

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Involvement in disease

Combined oxidative phosphorylation deficiency 29 (COXPD29)

Subcellular Location

Mitochondrion.

Protein Families

Thioredoxin family

Tissue Specificity

Widely expressed in adult (at protein level) and fetal tissues.

Database Links

HGNC: 17772

OMIM: 609063

KEGG: hsa:25828

STRING: 9606.ENSP00000216185

UniGene: Hs.211929

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

NOD-like receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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