Product Details

Uniprot No.

P13051

Target Names

UNG

Alternative Names

Uracil-DNA glycosylase (UDG) (EC 3.2.2.27), UNG, DGU UNG1 UNG15

Species Reactivity

Human

Immunogen

A synthesized peptide from human UNG protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Uracil-DNA glycosylase (UNG) serves as a critical guardian of genomic integrity, initiating base excision repair by removing uracil residues that arise from cytosine deamination or misincorporation of dUMP during DNA replication. This enzyme plays essential roles in maintaining DNA fidelity across dividing cells and has garnered significant attention in cancer research, immunology studies examining class switch recombination, and investigations into DNA damage response pathways.

This recombinant monoclonal antibody, generated from clone 8H1 in rabbit host, offers the reproducibility and consistency that demanding research applications require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures experimental comparability across collaborative projects.

Validation testing demonstrates reliable performance across multiple detection platforms. Western blot analysis of Jurkat whole cell lysate reveals a clean band at the predicted 35 kDa molecular weight, confirming specific detection of UNG protein at dilutions ranging from 1:500 to 1:2000. Immunohistochemical staining in paraffin-embedded human gastric cancer tissue shows effective antigen detection following citrate buffer retrieval, while immunofluorescence studies in HeLa cells demonstrate clear nuclear localization patterns consistent with UNG's role in DNA repair. Flow cytometry analysis further confirms utility for single-cell applications, with distinct population shifts observed in fixed and permeabilized HeLa cells.

This antibody provides researchers studying DNA repair mechanisms, mutagenesis, or cancer biology with a versatile tool validated across complementary techniques, enabling comprehensive characterization of UNG expression and localization in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA178691A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg) All lanes:UNG antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time：3min IHC image of CSB-RA178691A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA178691A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA178691A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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UNG Recombinant Monoclonal Antibody

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