USP11 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA934095A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), A375 whole cell lysate(30µg), A549 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: USP11 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time：2min Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA934095A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P51784

Target Names

USP11

Alternative Names

Deubiquitinating enzyme 11 antibody; Ubiquitin carboxyl-terminal hydrolase 11 antibody; Ubiquitin carboxyl-terminal hydrolase X linked antibody; ubiquitin specific peptidase 11 antibody; Ubiquitin specific protease 11 antibody; Ubiquitin thiolesterase 11 antibody; Ubiquitin-specific-processing protease 11 antibody; UBP11_HUMAN antibody; UHX1 antibody; USP11 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human USP11 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

USP11 is a deubiquitinating enzyme that plays critical roles in DNA damage repair, cell cycle regulation, and chromatin remodeling. By removing ubiquitin modifications from substrate proteins, USP11 influences the stability and function of key signaling molecules involved in genomic integrity and transcriptional control. This makes USP11 an important target for researchers investigating epigenetic mechanisms, nuclear signaling pathways, and the cellular response to genotoxic stress.

This recombinant monoclonal antibody, generated from clone 4C5, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format provides excellent sensitivity while the affinity-chromatography purification ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In western blot experiments, this antibody detects a clear band at the predicted molecular weight of 110 kDa across a diverse panel of human cell lines including HEK293, MCF-7, SH-SY5Y, A375, A549, and Jurkat cells at dilutions ranging from 1:500 to 1:5000. Cross-species reactivity has been confirmed through successful detection in mouse and rat brain tissue lysates, expanding the utility of this reagent for comparative studies and animal model research. Flow cytometry validation in SH-SY5Y cells demonstrates clear positive staining with appropriate signal separation from isotype controls, supporting intracellular detection applications.

Whether you are exploring USP11's role in DNA repair pathways, investigating its contributions to epigenetic regulation, or characterizing its expression across different experimental models, this antibody provides a dependable tool for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro). Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex.

Gene References into Functions

FASN-induced S6 kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in diffuse large B-cell lymphoma. PMID: 29483509
These findings reveal an important mechanism by which p21 can be stabilized by direct deubiquitylation, and they pinpoint a crucial role of the USP11-p21 axis in regulating cell-cycle progression and DNA damage responses. PMID: 29666278
show that USP11 is associated with the mitotic spindle, does not regulate SAC inactivation, but controls ubiquitination of RAE1 at the mitotic spindle, hereby functionally modulating its interaction with Nuclear Mitotic Apparatus protein (NuMA) PMID: 29293652
High USP11 expression is associated with cancer. PMID: 26919101
USP11 may contribute to the pathogenesis of pulmonary fibrosis by stabilization of TbetaRII and promotion of TGFbeta-1 signaling. PMID: 27853171
We demonstrated that, while depletion of XIAP attenuates cell transformation, elevated USP11 significantly promotes the tumor colony formation through stabilization of XIAP. PMID: 28040451
These findings elucidate deeply and extensively the mechanism of RNF8/RNF168 and USP11 to maintain the proper status of ubiquitylation gammaH2AX to repair double strand breaks. PMID: 26507658
novel insight into cross-talk between ubiquitin and SUMO and uncover USP11 and RNF4 as a balanced SUMO-targeted ubiquitin ligase/protease pair with a role in the DDR. PMID: 25969536
PML degradation mechanism through Notch/Hey1-induced repression of USP11 involved in brain tumour pathogenesis PMID: 24487962
USP11 is a novel regulator of p53, which is required for p53 activation in response to DNA damage PMID: 25471832
Our data support a model whereby USP11 domains outside the catalytic core domain serve as protein interaction or trafficking modules rather than a direct regulatory function of the proteolytic activity. PMID: 24724799
USP-11 is not a predictor of a pCR after anthracycline-taxane-containing NST for breast cancer. Low USP-11 expression was independently correlated with better survival outcomes. PMID: 23337751
USP11 augments TGFbeta signalling by deubiquitylating ALK5. PMID: 22773947
Ubiquitin specific protease 11 protein plays an important role in maintaining a delicate balance in TNFalpha-mediated inflammatory responses by being a part of Yin-Yang regulatory mechanism. PMID: 19874889
structural and functional characterization of the USP11 deubiquitinating enzyme PMID: 12084015
Results suggest that BRCA2 expression levels are regulated by ubiquitination in response to DNA damage and that USP11 participates in DNA damage repair functions within the BRCA2 pathway independently of BRCA2 deubiquitination. PMID: 15314155
the ectopic expression of IKKalpha into cells silenced for USP11 restores p53 expression, demonstrating that USP11 functions as an upstream regulator of an IKKalpha-p53 signaling pathway. PMID: 17897950
USP11 plays an important role in regulating the levels of E7 protein and subsequently affects the biological function of E7 as well as its contribution to cell transformation by HPV-16E7. PMID: 18408009

Hide All

Subcellular Location

Nucleus. Cytoplasm. Chromosome.

Protein Families

Peptidase C19 family

Database Links

HGNC: 12609

OMIM: 300050

KEGG: hsa:8237

STRING: 9606.ENSP00000218348

UniGene: Hs.171501

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address