Product Details

Uniprot No.

O00401

Target Names

WASL

Alternative Names

Neural Wiskott-Aldrich syndrome protein (N-WASP), WASL

Species Reactivity

Human

Immunogen

A synthesized peptide from human WASL protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

WASL, also known as Neural Wiskott-Aldrich syndrome protein (N-WASP), serves as a critical regulator of actin cytoskeleton dynamics, orchestrating the formation of branched actin networks essential for cell motility, endocytosis, and membrane protrusion. This protein acts as a key effector downstream of Cdc42 signaling, making it central to studies of cell migration, invasion, and intracellular pathogen motility. Researchers investigating cytoskeletal remodeling, cancer metastasis, or neuronal development will find WASL an informative target for understanding how cells coordinate their structural machinery.

This recombinant monoclonal antibody, clone 13F2, offers the reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human WASL, the antibody is produced using recombinant technology, ensuring sequence-defined consistency across lots. This eliminates the variability often encountered with traditional hybridoma-derived antibodies, allowing you to confidently compare results across extended studies or collaborative projects.

Validation data demonstrates reliable performance in immunofluorescence and flow cytometry applications. Immunofluorescence staining of SH-SY5Y neuroblastoma cells reveals clear cytoplasmic localization patterns at dilutions of 1:50 to 1:200, consistent with WASL's known distribution at sites of active actin remodeling. Flow cytometry analysis using A431 epidermoid carcinoma cells shows distinct positive signal separation from isotype control, confirming specific detection in this cell line at 1:100 dilution.

The antibody is supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C. Whether you are examining actin dynamics in cancer cell models, studying neuronal morphogenesis, or investigating host-pathogen interactions, this antibody provides a dependable tool for visualizing WASL in human cell systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis. Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear.

Gene References into Functions

N-WASP positively regulates demarcation membrane system development and proplatelet formation, and the Src family kinases in association with CDC42 regulate proplatelet formation through N-WASP PMID: 27685868
By using VCA nanobodiess, study shows the influence of N-WASps VCA domain in invadopodium formation and extracellular matrix degradation PMID: 28938008
Recruitment of Paxillin, Cdc42 and N-WASP is necessary for cell adhesion, migration and invasion induced by Estradiol in breast cancer cells. PMID: 27095481
This suggests that TOCA1 binding to Cdc42 is an early step in the Cdc42-dependent pathways that govern actin dynamics, and the differential binding affinities of the effectors facilitate a handover from TOCA1 to N-WASP, which can then drive recruitment of the actin-modifying machinery. PMID: 27129201
the Cdc42/Nwasp/stress fibers/YAP signal pathway may potentially play an important role in regulating podocyte apoptosis. Maintaining necessary Cdc42 would be one potent way to prevent proteinuria kidney diseases. PMID: 26986510
RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187
Nck was the most potent activator of N-WASP-driven actin assembly. PMID: 26554011
our results reveal a novel role for N-WASP in remodeling EC junctions, which is critical for monolayer integrity and function. PMID: 26070569
These results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in centronuclear myopathy and myotonic dystrophy pathophysiology. PMID: 25262827
PC1 modulates actin cytoskeleton rearrangements and directional cell migration through the Pacsin 2/N-Wasp complex. PMID: 24385601
Our observations suggest that ITSN1 is an important general regulator of Cdc42-, Nck- and N-WASP-dependent actin polymerisation PMID: 24284073
c-Src and NWASP play key roles in mediating the molecular pathogenesis of low oxygen-induced accelerated brain invasion by gliomas. PMID: 24069415
A gain-of-function approach for miR-142-3p revealed a down-regulation of N-Wasp expression accompanied by a decrease of mycobacteria intake, while a loss-of-function approach yielded the reciprocal increase of the phagocytosis process. PMID: 23760605
endocytosis by endothelial cells may represent a means of traversal of the blood vessel wall by yeast during disseminated candidiasis, and N-WASP may play a key role in the process. PMID: 23690407
N-WASP has a crucial proinvasive role in driving Arp2/3 complex-mediated actin assembly in cooperation with FAK at invasive cell edges, but WRC depletion can promote 3D cell motility. PMID: 23273897
NWASP activation by mycolactone underpins Buruli ulcer formation via modulation of the actin cytoskeleton. PMID: 23549080
Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. PMID: 23212915
Study portrays a role for Claudin-5 in cell motility involving the N-WASP signalling cascade indicating a possible role for Claudin-5 in the metastasis of human breast cancer. PMID: 22559840
results indicate that by mediating intensive F-actin accumulation at the sites of cell infiltration, WAVE2, N-WASP, and Mena are crucial for PI3K-dependent cell invasion induced by PDGF PMID: 21769917
N-WASP assists in the recruitment and localization of Tuba to the cell junction. PMID: 21677511
Perturbation of N-WASP-CK2 complex function showed that N-WASP controls the presence of F-actin at clathrin-coated structures. N-WASP-CK2 complex integrates in a single circuit different activities contributing to CME. PMID: 21610097
N-WASP plays a role in efficient assembly of kinetochores and attachment of microtubules to chromosomes, which is essential for accurate chromosome congression and segregation PMID: 21533546
N-WASP-WIRE serves as an integrator that couples actin nucleation with the subsequent steps of filament stabilization and organization necessary for zonula adherens integrity. PMID: 21785420
Recruitment of FAK and N-WASP is necessary for cell migration and invasion induced by 17beta-estradiol in breast cancer cells. PMID: 20880986
CFL1 and N-WASP may play an important role in the tumorigenesis of ESCC, and to be the candidate novel biomarkers for the diagnosis and prognosis of esophageal squamous cell carcinoma. PMID: 20095995
Cdc42 regulates the activity of IRSp53 by regulating the IRSp53-WIRE interaction as well as localization of the complex to plasma membrane to generate filopodia. PMID: 20678498
Cdc42 may influence endocytic membrane trafficking by regulating the formation and activity of the Toca-1/N-WASP complex. PMID: 20730103
Arg promotes actin-based protrusions in response to extracellular stimuli through phosphorylation of and physical interactions with N-WASp. PMID: 20146487
nuclear localization of N-WASP modulates Src kinase activity by regulating HSP90 expression PMID: 12871950
Expression of the WA fragment of N-WASP to block associated actin polymerization also inhibited C. parvum invasion. PMID: 15102814
Erk phosphorylation liberates the SH3 domain of cortactin from intramolecular interactions with proline-rich regions, causing it to synergize with WASP and N-WASP in activating the Arp2/3 complex PMID: 15169891
The cooperative actions of two distinct Cdc42 effectors, the N-WASP-WIP complex and Toca-1, are required for Cdc42-induced actin assembly. PMID: 15260990
NF2 tumor suppressor Merlin and the ERM proteins interact with N-WASP and regulate its actin polymerization function PMID: 15699051
Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity PMID: 16687395
We demonstrate that the interaction of N-WASP with the PSF-NonO complex can couple N-WASP with RNA polymerase II to regulate transcription. PMID: 16767080
a vesicle trafficking regulator Toca-1 regulates different aspects of neuronal morphology from N-WASP PMID: 16885158
CR16 and N-WASP are suggested to play important roles in spermatogenesis PMID: 17573773
N-WASP, a member of the N-WASP family may act as a tumour progression suppressor in human breast cancer and may therefore have significant clinical value in this condition. PMID: 17985201
These data constitute evidence that Toca-1 is required for N-WASP-mediated actin polymerization by Shigella flexneri in vivo. PMID: 18191793
of N-WASP and, subsequently, the Arp2/3 complex appears to be an important molecular signal for regulating spines and synapses PMID: 18430734
Wiskostatin induced defective cytokinesis does not occur through the inhibition of the N-WASP pathway. PMID: 18667055
Compared with controls, neuronal Wiskott-Aldrich syndrome protein expression in brains of intractable epilepsy patients was significantly higher. PMID: 18708039
the Toca-1-N-WASP complex can link filopodial formation to endocytosis PMID: 19213734
the exchange rate of N-WASP controls the rate of ARP2/3-complex-dependent actin-based motility by regulating the extent of actin polymerization by antagonizing filament capping PMID: 19262673

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Subcellular Location

Cytoplasm, cytoskeleton. Nucleus. Cytoplasm.

Database Links

HGNC: 12735

OMIM: 605056

KEGG: hsa:8976

STRING: 9606.ENSP00000223023

UniGene: Hs.143728

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Code	CSB-RA196026A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of SH-SY5Y cell with CSB-RA196026A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA196026A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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WASL Recombinant Monoclonal Antibody

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