XPO5 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA293843A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: Exportin 5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time：2min IHC image of CSB-RA293843A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA293843A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA293843A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA293843A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9HAV4

Target Names

XPO5

Alternative Names

Exportin-5 (Exp5) (Ran-binding protein 21), XPO5, KIAA1291 RANBP21

Species Reactivity

Human

Immunogen

A synthesized peptide from human XPO5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Exportin-5 (XPO5) serves as a critical nuclear export receptor responsible for transporting precursor microRNAs from the nucleus to the cytoplasm, where they undergo further processing into mature regulatory molecules. This function positions XPO5 at a pivotal junction in the microRNA biogenesis pathway, making it an essential target for researchers investigating gene silencing mechanisms, RNA processing disorders, and the dysregulation of non-coding RNAs in disease states.

This recombinant monoclonal antibody, generated from clone 21D11, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant production ensures a sequence-defined antibody with minimal lot-to-lot variation, researchers can confidently build upon previous results without concerns about reagent drift affecting their data over time.

Validation across multiple applications demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis confirms specific detection of XPO5 at the expected 136 kDa molecular weight across diverse human cell lines including HeLa, K562, HT-29, and Jurkat cells, providing confidence when working with various cellular models. Immunohistochemistry staining has been successfully performed on paraffin-embedded human breast cancer and testis tissues, supporting investigations into XPO5 expression patterns in both malignant and normal tissue contexts. For subcellular localization studies, immunofluorescence staining in MCF-7 cells and flow cytometry analysis in SH-SY5Y neuroblastoma cells extend the antibody's utility to single-cell resolution techniques.

Whether you are exploring microRNA pathway components in cancer biology, investigating nuclear transport mechanisms, or characterizing XPO5 expression across tissue types, this antibody provides a reliable foundation for generating meaningful, reproducible data in your research program.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.; Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.; (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.

Gene References into Functions

DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers..), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048). PMID: 29683064
XPO5 might have a dual role in promoting or inhibiting tumor growth in different cancer tissue types. PMID: 29699373
overexpression of XPO5 causes dysregulation in the miRNA processing mechanism, resulting in a shift in protein stability via lower ubiquitination and hyper N-linked glycosylation, as well as conferring a growth advantage by suppressing the DNA damage response. PMID: 28881308
data strongly strengthened the notion that phosphorylation of XPO5 by ERK downregulates miRNA expression in clinical samples and is correlated with poor clinical outcomes of HCC patients PMID: 28500493
Aberrant XPO5 expression is important for the maturation of miRNAs and the malignant behavior of melanoma cells. High abundance of XPO5 in melanoma leads to enhanced survival, proliferation and metastasis. PMID: 27556702
XPO5 acts like an oncogene in colorectal cancer by regulating the expression of miRNAs and may be a potential therapeutic target in colorectal cancer PMID: 27553833
one SNP in XPO5 extron (rs2257082) was significantly associated with lead-poisoning; there were no significant association between the other six SNPs and the blood lead levels; polymorphism rs2257082 could be used to distinguish lead-resistant and lead-susceptible populations PMID: 28042866
SNP rs11077 influences the expression of XPO5, and this SNP could also be a potential biomarker for the diagnosis of thyroid cancer, especially in Chinese population PMID: 28383405
findings suggest that XPO5 functions as a potential tumor suppressor in the development and progression of HCC as well as a promising molecular target for HCC therapy PMID: 27000860
The microRNA-related single-nucleotide polymorphisms rs11077 of XPO5 may be independently connected with the prognosis and chemotherapy response of advanced non-small-cell lung cancer patients PMID: 26358254
XPO5 role in microRNA biogenesis PMID: 26976605
NUP93 and exportin 5 interact with the signaling protein SMAD4 and that NUP93 mutations abrogated interaction with SMAD4 PMID: 26878725
Variations in XPO5 rs11077 of Korean patients are significantly associated with their risk of colorectal cancer. PMID: 26147304
Knockdown of exportins 4, 5, and 7 altered thyroid hormone receptor shuttling dynamics, and when exportins 5 and 7 were overexpressed, TR distribution shifted toward the cytosol. PMID: 25911113
eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. PMID: 24209753
data demonstrate that the exportin 5 (XPO5) rs2257082 T variant allele occurs more frequently in primary ovarian insufficiency (POI)patients than in controls, suggesting that this allele may be associated with increased POI risk PMID: 23549446
The study identifies a miR-138-RMND5A-Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells. PMID: 24057215
Dicer, Drosha, and Exportin 5 genes were up-regulated in bladder urothelial carcinoma compared to normal urothelium. Silencing these genes induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma cells. PMID: 22766726
overall survival was significantly longer in multiple myeloma patients with C/C or A/C variant in the XPO5 gene single nucleotide polymorphism site rs11077 PMID: 22539802
Exportin-5 controls Dicer1 expression post-transcriptionally. PMID: 21297638
Data show a requirement for the export receptor Exportin 5 in nuclear export of 60S subunits in human cells, and that Exp5 binds to pre-60S particles in a RanGTP-dependent manner. PMID: 21048991
The restoration of XPO5 functions reverses the impaired export of pre-miRNAs and has tumor-suppressor features. PMID: 20951941
Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. PMID: 12426392
Eukaryotic elongation factor 1A (eEF1A) and tRNA are RanGTP-dependent binding partners of exportin-5 (Exp5). Exp5 stimulates nuclear export of eEF1A and tRNA. PMID: 12426393
exportin-5 has a role in nuclear export of ILF3 (interleukin enhancer binding factor 3) PMID: 14570900
exportin-5 is key to microRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps PMID: 14631048
exportin-5 has a role in exporting JAZ from the cell nucleus PMID: 15254228
Each of the dsRBDs in ADAR1 interacts with the export factor exportin-5. PMID: 19124606
2.9 angstrom structure of the pre-miRNA nuclear export machinery formed by pre-miRNA complexed with Exp-5 and RanGTP; RNA recognition by Exp-5:RanGTP does not depend on RNA sequence, implying that Exp-5:RanGTP can recognize a variety of pre-miRNAs PMID: 19965479

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Subcellular Location

Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm.

Protein Families

Exportin family

Tissue Specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 17675

OMIM: 607845

KEGG: hsa:57510

STRING: 9606.ENSP00000265351

UniGene: Hs.203206

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