Product Details

Uniprot No.

P07947

Target Names

YES1

Alternative Names

Tyrosine-protein kinase Yes (EC 2.7.10.2) (Proto-oncogene c-Yes) (p61-Yes), YES1, YES

Species Reactivity

Human

Immunogen

A synthesized peptide from human YES1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2G10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

YES1 is a non-receptor tyrosine kinase belonging to the Src family, playing essential roles in signal transduction pathways that regulate cell proliferation, differentiation, and survival. As a proto-oncogene, YES1 has garnered significant research attention for its involvement in various malignancies, where its aberrant activation contributes to tumor progression and metastasis. Understanding YES1 signaling dynamics is crucial for researchers investigating cancer biology, cellular adhesion mechanisms, and therapeutic targeting strategies.

This recombinant monoclonal antibody, clone 2G10, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a sequence-defined approach, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit host origin provides excellent affinity characteristics, while the monoclonal nature ensures recognition of a single epitope on the YES1 protein, generated against a synthesized peptide from the human sequence.

Validation studies demonstrate reliable performance in flow cytometry applications, with successful detection of YES1 in MCF-7 human breast cancer cells. Using intracellular staining protocols with formaldehyde fixation and Triton X-100 permeabilization, the antibody produced clear positive signal separation from isotype control at a 1:100 dilution, confirming specificity for endogenous YES1 expression. The antibody is also validated for ELISA applications, offering flexibility across different experimental platforms.

Supplied in a stabilized liquid format with glycerol for convenient storage at -20°C or -80°C, this antibody is well-suited for researchers investigating Src family kinase signaling, oncogenic transformation mechanisms, or screening potential therapeutic interventions targeting YES1-dependent pathways in human cancer models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Gene References into Functions

The downregulation of miR-199a contributes to paclitaxel (PTX) resistance in prostate cancer. YES1 mediates the regulation of miR-199a in prostate cancer PTX resistance. PMID: 29204706
findings identify acquired amplification of YES1 as a recurrent and targetable mechanism of resistance to EGFR inhibition in EGFR-mutant lung cancers and demonstrate the utility of transposon mutagenesis in discovering clinically relevant mechanisms of drug resistance. PMID: 29875142
Study on the role of miR-140-5p in inhibiting tumorigenesis in gastric cancer thru targeting YES proto-oncogene 1(YES1). PMID: 28818100
Our results suggest that H19 functions as a competitive endogenous RNA (ceRNA) by acting as a sink for miR-17-5p, revealing a potential ceRNA regulatory network involving H19 and miR-17-5p with a role in the modulation of YES1 expression PMID: 27093644
c-Yes plays an important role in migration and invasion of epithelial ovarian cancer. PMID: 27289010
Up-regulation of miR-210 inhibits hepatocellular carcinoma cell proliferation. Yes1 is a target of miR-210 and affects cell proliferation in HCC. PMID: 26676187
findings indicate that miR-203 induces the apoptosis of KB cells by directly targeting Yes-1, suggesting its application in anti-cancer therapeutics PMID: 25910964
Results demonstrate a unique role for Yes in phosphorylation of FAK and in promoting PCa metastasis. Therefore, phosphorylated FAK Y861 and increased Yes expression may be predictive markers for PCa metastasis. PMID: 25868388
results indicated that miR-17-5p might play a role in human ovarian cancer by up-regulating YES1 expression. PMID: 25561420
Increased YES/SFK activation might serve as a clinical biomarker for predicting tumor resistance to IGF-1R inhibition. PMID: 25925378
YES1 and YAP transcript levels were higher in liver metastases of patients with colon cancer after 5FU-based neoadjuvant chemotherapy. PMID: 24323901
YES kinase as a proximal glucose-specific signal in the Cdc42-signaling cascade. PMID: 24610809
we identified CRKL/YES as critical interrelated pathways necessary for rhabdomyosarcoma cell growth and survival and suggest a potential therapeutic role of SRC family kinase inhibition in the treatment of rhabdomyosarcoma. PMID: 23318429
Expression of HPV type 16 E7 resulted in increase in Src and Yes proteins level, but did not alter the level of Fyn. PMID: 23497302
Taken together, our findings demonstrate that Yes and Lyn phosphorylate EGFR at Y1101, which influences EGFR nuclear translocation in this model of cetuximab resistance. PMID: 22430206
study concludes that Yes is a central mediator for malignant mesothelioma cell growth that is not shared with other Src family kinases such as c-Src PMID: 22948717
c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src. PMID: 21390316
Functional activation of Src family kinase yes protein is essential for the enhanced malignant properties of human melanoma cells expressing ganglioside GD3. PMID: 21454696
Yes-associated protein may play important roles in different pathways in distinct lung tumor subtypes. PMID: 21190720
c-Yes was expressed in malignant melanoma, and squamous cell carcinoma type skin neoplasms. PMID: 20796316
Cloning of the complete coding sequence of the cDNA of the Csk and Yes tyrosine kinases genes of the human lymphocytes and retina have been carried out. PMID: 19757166
Both YES and STAT1 were verified as direct miR-145 targets PMID: 20098684
Study identified the previously reported pathogenic mutation of NTRK3 in a KRAS/BRAF wild-type tumor and 2 somatic mutations in the Src family of kinases (YES1 and LYN) that would be expected to cause structural changes. PMID: 19893451
Data show that found that IFIH1, a susceptibility gene of type 1 diabetes, interacts with YES1, which plays a role in glucose transport. PMID: 19797678
cAMP activates while Ca(2+) inhibits human sperm c-yes kinase activity PMID: 12080032
QM binds to c-yes at the SH3 domain in tumor cell lines PMID: 12138090
This review summarizes the potential functions of c-Yes and its ability to modulate signals that are distinct from c-Src. PMID: 12456296
Src family kinase Yes triggers hyperosmotic activation of the epidermal growth factor receptor and CD95 PMID: 15039424
activation of c-Yes is important for maintaining embryonic stem cells in an undifferentiated state PMID: 15148312
c-Yes 3'-UTR contains at least three newly identified adenine/uridine-rich elements (AREs) which are bound specifically by ARE-binding proteins HuR and AUF1. PMID: 16289864
Vascular endothelial growth factor (VEGF)-induced cell migration is significantly decreased in Yes-deficient retinal microvascular endothelial cells. PMID: 16400523
activation of the protooncogene product c-Yes may play a significant role in the malignant transformation of hepatocytes PMID: 17007035
The results were confirmed at the level of mRNA and protein, and suggested that four genes (OPCML, RNASE1, YES1 and ACK1) could play a key role in the tumorigenesis and metastasis of gastric cancer. PMID: 17109515
Nucleus-located c-Yes may be a useful marker to detect early-stage hepatocellular carcinoma. PMID: 17143518
Amyloid aggregation by an SH3 domain from the Src family. PMID: 17418139
Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase. PMID: 17692281
c-Yes induction results in increased colorectal carcinoma cell motility but did not promote proliferation. In later stages of colorectal carcinogenesis, elevations in c-Yes levels/activity may promote cancer spread & metastasis rather than tumor growth. PMID: 17898870
Binding of CD95 Ligand to CD95 on glioblastoma cells recruit Yes and the p85 subunit of phosphatidylinositol 3-kinase to CD95, which signal invasion via the glycogen synthase kinase 3-beta pathway and subsequent expression of matrix metalloproteinases. PMID: 18328427
These results suggest that SFK trafficking is specified by the palmitoylation state in the SH4 domain. PMID: 19258394

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Subcellular Location

Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytosol. Note=Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, SRC subfamily

Tissue Specificity

Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes i

Database Links

HGNC: 12841

OMIM: 164880

KEGG: hsa:7525

STRING: 9606.ENSP00000324740

UniGene: Hs.194148

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Code	CSB-RA286515A0HU
Size	US$210
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Image	Overlay Peak curve showing MCF-7 cells stained with CSB-RA286515A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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YES1 Recombinant Monoclonal Antibody

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