Flag-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000156
Product Type Monoclonal Antibody
Former Code(s) CSB-MA220517
Size US$167
Image
  • IP antibody use: 5µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with Flag-tag fusion protein
    Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 4: IP (transfected 293 + anti- Flag mAb + Protein G agarose)
    Lane 5: IP (transfected 293 + Protein G)
    Lane 6: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)

  • 1µg Flag fusion protein + Primary antibody dilution at 1:10000

Immunogen Synthetic Peptide
Raised in Mouse
Tested Applications ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Isotype IgG
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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