Flag-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000156
Size US$167
Image
  • IP antibody use: 5µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with Flag-tag fusion protein
    Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 4: IP (transfected 293 + anti- Flag mAb + Protein G agarose)
    Lane 5: IP (transfected 293 + Protein G)
    Lane 6: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)

  • 1µg Flag fusion protein + Primary antibody dilution at 1:10000

Product Details

Target Names Flag-Tag
Raised in Mouse
Species Reactivity N/A
Immunogen Synthetic Peptide
Conjugate Non-conjugated
Isotype IgG
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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