6*His Monoclonal Antibody, Biotin conjugated

Widely adopted as the gold-standard affinity handle in recombinant protein research, the 6×His tag enables rapid one-step purification via immobilized metal-ion chromatography (Ni²⁺/Co²⁺) while simultaneously serving as a compact, non-disruptive epitope for immunological detection. When paired with a biotin-conjugated detection reagent, the system gains an additional layer of signal amplification through streptavidin–enzyme conjugates, making it exceptionally well-suited for high-sensitivity screening and quantitative immunoassays.

The Mouse Anti-6×His Monoclonal Antibody, Biotin conjugated (CSB-MA000011D0m, IgG2a) takes a distinctive approach to immunogen design: rather than relying on a single synthetic peptide, it is raised against a mixture of various His-tagged proteins. This strategy broadens epitope coverage across different protein contexts and linker configurations, enhancing the antibody's ability to recognize 6×His fusions irrespective of the surrounding scaffold. Produced via hybridoma technology and rigorously purified by Protein G chromatography to >95% purity, this reagent delivers exceptional monospecificity and batch-to-batch consistency, with the biotin moiety covalently attached to provide a ready-to-use detection handle.

Validated specifically for Enzyme-Linked Immunosorbent Assay (ELISA), this biotinylated format shines in sandwich ELISA architectures—where it can serve as either the capture or detection layer when paired with a complementary anti-His partner—as well as in high-throughput screening of expression libraries and monitoring of purification eluate fractions. Its IgG2a isotype and liquid formulation ensure straightforward integration into existing workflows, while the biotin conjugation eliminates the need for secondary antibody steps, streamlining assay design and reducing background.

This reagent is a strategic complement to conventional unconjugated anti-His antibodies, offering researchers a purpose-built tool for scenarios where signal amplification, assay speed, or multiplexing flexibility are priorities. Whether deployed in recombinant protein quantification, purification workflow QC, or high-content screening campaigns, the Biotin-conjugated 6×His Monoclonal Antibody delivers the sensitivity and reliability expected from a CUSABIO tag antibody series member.