TUBG1 Monoclonal Antibody

Gamma-tubulin (encoded by the TUBG1 gene) is a fundamental structural protein primarily localized at the centrosome, where it serves as the core component of the gamma-tubulin ring complex (γ-TuRC). This complex is indispensable for microtubule nucleation and organization, playing pivotal roles in cell division, intracellular transport, and maintaining cellular architecture.

The Mouse Anti-TUBG1 Monoclonal Antibody (CSB-MA080295) is specifically developed to recognize Gamma-tubulin. Generated using a synthetic peptide corresponding to the Gamma Tubulin sequence and affinity-purified from mouse ascites, this antibody achieves exceptional purity (>95%) and ensures high lot-to-lot consistency. Validated for use in ELISA, Western Blot (WB), and Immunohistochemistry (IHC), it demonstrates broad species reactivity across Human, Rat, and Mouse samples.

Rigorous in-house validation underscores its reliability as a specialized loading control and diagnostic reagent. In IHC analyses of paraffin-embedded human colon and breast carcinoma tissues (diluted 1:200), the antibody produces distinct, granular cytoplasmic staining in tumor cells, confirming its efficacy in detecting native protein expression in complex clinical samples. Furthermore, Western Blot analysis across diverse lysates (Jurkat, HeLa, Mouse brain, Rat brain) at a 1:1000 dilution reveals a crisp, single band at approximately 50 kD. This consistent cross-species performance makes it an ideal internal reference for normalizing protein loading in Western Blots, particularly in experiments where traditional loading controls (like β-Actin or GAPDH) may be subject to fluctuation, such as in highly proliferative cells or metabolic studies.

As a trusted immunological tool, this CUSABIO monoclonal antibody is backed by comprehensive technical support. It empowers researchers to obtain accurate, reproducible data in studies ranging from cell cycle regulation and cancer biomarker discovery to neurobiology, ensuring dependable results for their cutting-edge research.