mCherry-Tag Monoclonal Antibody

mCherry, derived from the DsRed constituent of Discosoma coral, is a monomeric red fluorescent protein prized for its superior photophysical properties, including fast maturation kinetics and high pH resistance. As a widely adopted genetic tag, it is extensively utilized to investigate protein localization, trafficking, and dynamic interactions within living cells.

The Mouse Anti-mCherry-Tag Monoclonal Antibody (CSB-MA000165, IgG isotype) is expertly generated via hybridoma technology using highly purified recombinant mCherry protein as the immunogen. This ensures exceptional specificity and robust monospecificity for the target epitope, effectively eliminating cross-reactivity with other common fluorescent proteins such as GFP or RFP.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB), this reagent demonstrates remarkable sensitivity and binding avidity. As clearly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 1 µg of mCherry fusion protein at impressively high primary antibody working dilutions of 1:5,000 and 1:10,000. The presence of sharp, densely staining bands at both concentrations—completely free from background interference or non-specific artifacts—unequivocally confirms the antibody's outstanding immunoreactivity and exceptional signal-to-noise ratio. Such potent performance at minimal concentrations offers significant cost-efficiency, making it an ideal reagent for high-throughput screening and detailed molecular characterization.

This monoclonal antibody serves as an indispensable tool for researchers engaged in advanced cell biology and molecular imaging. It provides a definitive method for verifying mCherry-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of recombinant protein purification. With its proven reliability, the mCherry-Tag Monoclonal Antibody consistently delivers high-fidelity results, effectively supporting robust research in live-cell imaging, protein engineering, and subcellular localization studies.