Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2)

Code CSB-CF022654HU
Size Pls inquire other sizes
Source in vitro E.coli expression system

Product Details

Target Names SRD5A2
Uniprot No. P31213
Species Homo sapiens (Human)
Expression Region 1-254
Target Protein Sequence MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Q&A and Customer Reviews

 Q&A
Q:

Regarding your protein CSB-CF022654HU, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, how long after the expression begins would you know what kind of tag this protein is ultimately going to have?
Lastly, what is the expected purity?

A:
Very nice to receive your inquiry.
Expression Region: 1-254 aa; Full length.
Tag information:Tag type will be determined during the manufacturing process. (The expected tag is N-terminal 10xHis-tagged)
Sequence:

MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF


The expected tag of CSB-CF022654HU is N-terminal 10xHis-tagged, if you have any special requirement for the tag, you can communicate with us in advance.
Generally, the tag of this protein can be confirmed in 15-20 working days after we receive your order.
Regarding the purity, the minimum purity that we can guarantee is 85%, which means that the purity of final protein will be above 85%.
If the customer requires much higher purity than 85%, pls remark this requirement in the order, we can try our best, but as we have never expressed, so we can't guarantee it can reach 90% or 95%.
Partial protein to recommend:
Recombinant Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2),partial
CSB-YP022654HU1 >> Yeast
CSB-EP022654HU1 >> E.coli
CSB-BP022654HU1 >> Baculovirus
CSB-MP022654HU1 >> Mammalian cell
Expression Region: 29-71aa; Partial.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP, BP, MP: N-terminal 6xHis-tagged; EP: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged.
Sequence:

KPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQ

 Customer Reviews

Target Data

Function Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Involvement in disease Pseudovaginal perineoscrotal hypospadias (PPSH)
Subcellular Location Microsome membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Steroid 5-alpha reductase family
Tissue Specificity Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Database Links

HGNC: 11285

OMIM: 264600

KEGG: hsa:6716

UniGene: Hs.458345

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