Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

CLDN11

Uniprot No.

O75508

Research Area

Immunology

Alternative Names

(Oligodendrocyte-specific protein)

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

1-207aa

Target Protein Sequence

MVATCLQVVGFVTSFVGWIGVIVTTSTNDWVVTCGYTIPTCRKLDELGSKGLWADCVMATGLYHCKPLVDILILPGYVQACRALMIAASVLGLPAILLLLTVLPCIRMGQEPGVAKYRRAQLAGVLLILLALCALVATIWFPVCAHRETTIVSFGYSLYAGWIGAVLCLVGGCVILCCAGDAQAFGENRFYYTAGSSSPTHAKSAHV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

23.5 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Human Claudin-11 (CLDN11) is a high-purity, full-length protein produced for Immunology research. It is expressed in an in vitro E. coli expression system, corresponding to the complete amino acid sequence 1-207 of human CLDN11 with an N-terminal 10xHis tag, yielding a molecular weight of 23.5 kDa. A key feature is its verified high quality: SDS-PAGE analysis under reducing conditions shows a single, sharp predominant band at the expected position of approximately 25 kDa, confirming a purity greater than 85%. This ready-to-use reagent provides a reliable and defined antigen for antibody production and immunoassay development targeting the tight junction protein CLDN11.

Target Background

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Gene References into Functions

expression of claudin-11 in cutaneous squamous cell carcinoma (cSCC) cells depended on the activity of p38delta MAPK; knock-down of claudin-11 enhanced cSCC cell invasion PMID: 27992079
Authors demonstrated that CLDN11 promoter hypermethylation is a frequent event in LSCC, and contributes to metastasis and progression of LSCC. PMID: 28743857
The expression of claudin-11, -23 was remarkably downregulated in gastric cancer. PMID: 28350854
Claudin-11 might represent the essential component of the blood-testis barrier in human. PMID: 27486954
the expression of miR-99b was inversely correlated with CLDN11 levels . These findings suggest that a high level of miR-99b expression is an independent prognostic factor and correlates with poor survival of patients with Hepatocellular carcinoma (HCC) PMID: 26134929
CLDN11 is an epigenetic biomarker for malignancy in dysplastic nevus and melanoma. PMID: 24999589
these data suggest that cancer cells may induce CLDN11 overexpression and subsequent collective migration of peritumoral CAFs via TGF-beta secretion. PMID: 24268521
The expression of claudin-11 was up regulated in gastric cancer tissue. PMID: 23919729
The spatial organization of claudin-11 and connexin-43 is altered in men with primary seminiferous tubule failure. PMID: 23706332
disorganization of claudin-11 expression in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis. PMID: 22951003
Treatment with 3-deazaneplanocin A, an inhibitor of H3K27 methyltransferase, attenuated CLDN11 induction by serum stimulation in parallel with sustained miR-1275 expression PMID: 22736761
claudin-11 may have a role in preventing cancer progression and may serve as a therapeutic target in reducing metastasis PMID: 21468549
late spermatogenic wave may negatively regulate claudin-11 gene activation and the subcellular localization of claudin-11 in Sertoli cells, thus altering the blood testis barrier in the human testis PMID: 20850723
Claudins 11,expression in meningiomas. PMID: 20546350
hypermethylation of CLDN11, leading to downregulated expression, contributes to gastric carcinogenesis by increasing cellular motility and invasiveness PMID: 19956721
Contribution of the tight junction protein CLDN11 to barrier function in endothelial cells is novel and may reflect hemodynamic requirements of the corpus cavernosum. PMID: 19622796
Data demonstrate that in rhesus monkeys immune responses directed at human OSP are encephalitogenic, leading to inflammatory responses throughout the central nervous system and to selective demyelination of the optic nerve. PMID: 18412169
the disruption of the blood-testis barrier is related to a dysfunction of claudin-11 and not to a failure of its expression. PMID: 19241088

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Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Protein Families

Claudin family

Database Links

HGNC: 8514

OMIM: 601326

KEGG: hsa:5010

STRING: 9606.ENSP00000064724

UniGene: Hs.31595

Code	CSB-CF005492HU
Abbreviation	Recombinant Human CLDN11 protein
MSDS	MSDS Datasheet
Size	$1620
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Claudin-11 (CLDN11)

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