Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
ASPRV1; SASP; Retroviral-like aspartic protease 1; Skin-specific retroviral-like aspartic protease; SASPase; Skin aspartic protease; TPA-inducible aspartic proteinase-like protein; TAPS
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
191-326aa
Target Protein Sequence
SMGKGYYLKGKIGKVPVRFLVDSGAQVSVVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVANASAEEAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLE
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This highly advanced Recombinant Human ASPRV1 protein can be used in the field of cell biology research. ASPRV1, also known as Retroviral-like aspartic protease 1, Skin-specific retroviral-like aspartic protease, or TPA-inducible aspartic proteinase-like protein, is a crucial component involved in various cellular processes. Our recombinant protein is produced using an innovative in vitro E.coli expression system, ensuring its exceptional quality and functionality. It encompasses the full length of the mature ASPRV1 protein, spanning amino acids 191 to 326, providing comprehensive insights into its biological functions and regulatory mechanisms.
For ease of detection and purification, the Recombinant Human ASPRV1 protein is equipped with an N-terminal 10xHis tag and a C-terminal Myc tag. These tags enhance the solubility and simplifies the downstream applications of the protein. With a purity level of greater than 85% as determined by SDS-PAGE, you can have confidence in the reliability and consistency of this protein for your research needs. The Recombinant Human ASPRV1 is available in both liquid and lyophilized powder forms, providing flexibility in storage and experimental setups. Explore the vast potential of this protein in unraveling the intricate mechanisms of cellular biology and uncovering novel insights into cellular pathways and protein interactions.