Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ASPRV1

Uniprot No.

Q53RT3

Research Area

Cell Biology

Alternative Names

ASPRV1; SASP; Retroviral-like aspartic protease 1; Skin-specific retroviral-like aspartic protease; SASPase; Skin aspartic protease; TPA-inducible aspartic proteinase-like protein; TAPS

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

191-326aa

Target Protein Sequence

SMGKGYYLKGKIGKVPVRFLVDSGAQVSVVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVANASAEEAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

19.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This highly advanced Recombinant Human ASPRV1 protein can be used in the field of cell biology research. ASPRV1, also known as Retroviral-like aspartic protease 1, Skin-specific retroviral-like aspartic protease, or TPA-inducible aspartic proteinase-like protein, is a crucial component involved in various cellular processes. Our recombinant protein is produced using an innovative in vitro E.coli expression system, ensuring its exceptional quality and functionality. It encompasses the full length of the mature ASPRV1 protein, spanning amino acids 191 to 326, providing comprehensive insights into its biological functions and regulatory mechanisms.

For ease of detection and purification, the Recombinant Human ASPRV1 protein is equipped with an N-terminal 10xHis tag and a C-terminal Myc tag. These tags enhance the solubility and simplifies the downstream applications of the protein. With a purity level of greater than 85% as determined by SDS-PAGE, you can have confidence in the reliability and consistency of this protein for your research needs. The Recombinant Human ASPRV1 is available in both liquid and lyophilized powder forms, providing flexibility in storage and experimental setups. Explore the vast potential of this protein in unraveling the intricate mechanisms of cellular biology and uncovering novel insights into cellular pathways and protein interactions.

Target Background

Function

Protease responsible for filaggrin processing, essential for the maintenance of a proper epidermis organization.

Gene References into Functions

results failed to find an association between ASPRV1 gene mutations and atopic eczema or clinically dry skin in the European populations studied PMID: 22318384
SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the stratum corneum. PMID: 21542132
Identification and characterization of a new member of the aspartic proteases family, a unique epidermal retroviral-like protease, Skin ASpartic Protease. PMID: 16098038
Taps expression is negatively associated with dedifferentiation and malignant progression in squamous cell carcinomas of the skin. PMID: 16565508

Subcellular Location

Membrane; Single-pass membrane protein.

Tissue Specificity

Expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles. In psoriatic skin, expressed throughout the stratum corneum. In ulcerated skin, expressed in the stratum granulosum of intact epidermis but almost absent f

Database Links

HGNC: 26321

OMIM: 611765

KEGG: hsa:151516

STRING: 9606.ENSP00000315383

UniGene: Hs.516253

Code	CSB-CF684475HU
Abbreviation	Recombinant Human ASPRV1 protein
MSDS	MSDS Datasheet
Size	$878
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF684475HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASPRV1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF684475HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ASPRV1.
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Recombinant Human Retroviral-like aspartic protease 1 (ASPRV1)

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