Recombinant Human Stearoyl-CoA desaturase 5(SCD5)

Code CSB-CF020803HU
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names SCD5
Uniprot No. Q86SK9
Alternative Names SCD5; ACOD4; SCD2; SCD4; Stearoyl-CoA desaturase 5; Acyl-CoA-desaturase 4; HSCD5; Stearoyl-CoA 9-desaturase; Stearoyl-CoA desaturase 2
Species Homo sapiens (Human)
Expression Region 1-330
Target Protein Sequence MPGPATDAGKIPFCDAKEEIRAGLESSEGGGGPERPGARGQRQNIVWRNVVLMSLLHLGA VYSLVLIPKAKPLTLLWAYFCFLLAALGVTAGAHRLWSHRSYRAKLPLRIFLAVANSMAF QNDIFEWSRDHRAHHKYSETDADPHNARRGFFFSHIGWLFVRKHRDVIEKGRKLDVTDLL ADPVVRIQRKYYKISVVLMCFVVPTLVPWYIWGESLWNSYFLASILRYTISLNISWLVNS AAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDF MCWLGLATDRKRATKPMIEARKARTGDSSA
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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 Q&A
Q:

Regarding your protein CSB-CF020803HU, could you please advise on the lead time, the sequencing and the expected tag information ?
Also, I was wondering if the expression is carried out in the presence of detergents and or lipids?
Thanks!

A:
Thanks for your inquiry.
This is a transmembrane protein, which contains 4 transmembrane domains, we can only provide the full length with cell free expression system and provide the partial protein with four regular expression systems.
Recombinant Human Stearoyl-CoA desaturase 5(SCD5)
CSB-CF020803HU
Expression Region: 1-330 aa; Full length.
Tag information:Tag type will be determined during the manufacturing process. (The expected tag is N-terminal 10xHis-tagged)
Sequence:

MPGPATDAGKIPFCDAKEEIRAGLESSEGGGGPERPGARGQRQNIVWRNVVLMSLLHLGAVYSLVLIPKAKPLTLLWAYFCFLLAALGVTAGAHRLWSHRSYRAKLPLRIFLAVANSMAFQNDIFEWSRDHRAHHKYSETDADPHNARRGFFFSHIGWLFVRKHRDVIEKGRKLDVTDLLADPVVRIQRKYYKISVVLMCFVVPTLVPWYIWGESLWNSYFLASILRYTISLNISWLVNSAAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKPMIEARKARTGDSSA


For the cell free expression system we can provide liposomal nanodiscs, but we can't guarantee to form a stable nanodiscs-PIP2-1 complex.We can try it, if it's failed, we will choose the detergent, and the detergent will be shown in the COA.
Usually, We will use detergent for the membrane proteins extraction, dissolving and purification. The final product is provided in a soluble form.
Partial protein expressed by the regular four expression systems:
Recombinant Human Stearoyl-CoA desaturase 5(SCD5),partial
CSB-YP020803HU1 >> Yeast
CSB-EP020803HU1 >> E.coli
CSB-BP020803HU1 >> Baculovirus
CSB-MP020803HU1 >> Mammalian cell
Expression Region: 239-330aa; Fragment at the C-terminal.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

NSAAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKPMIEARKARTGDSSA

Target Background

Function
Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Involved in neuronal cell proliferation and differentiation through down-regulation of EGFR/AKT/MAPK and Wnt signaling pathways.
Gene References into Functions
  1. SCD5 appears to functionally connect tumour cells and the surrounding stroma toward modification of the tumour microenvironment, with consequences on tumour spread and resistance to treatment. PMID: 25802234
  2. The protective effect of TO901317 was lost after gene silencing of Scd-1/-2, thereby confirming that the protective effect of TO901317 is mediated by Scd-1/-2. PMID: 23867797
  3. Data suggest that, by a coordinated modulation of key lipogenic pathways and transduction signaling cascades, SCD5 participates in the regulation of neuronal cell growth and differentiation. PMID: 22745828
  4. SCD4 demonstrates tissue-specific regulation by leptin and dietary factors PMID: 12815040
  5. The identification and characterization of SCD2, and its relationship to human SCD1 and mouse SCD2, are reported. PMID: 15610069
  6. In this paper, we describe for the first time a second SCD isoform in cattle, which appears to be an ortholog of human SCD5 rather than a homolog of bovine SCD1 or any of the described murine SCD isoforms PMID: 17468887

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Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families Fatty acid desaturase type 1 family
Tissue Specificity Detected in fetal brain, and at lower levels in fetal kidney. Detected in adult brain and pancreas, and at lower levels in kidney and lung. Expressed in spiral ganglion cells and the organ of Corti of fetal cochlea.
Database Links

HGNC: 21088

OMIM: 608370

KEGG: hsa:79966

STRING: 9606.ENSP00000316329

UniGene: Hs.379191

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