Thanks for your inquiry.
This is a transmembrane protein, which contains 4 transmembrane domains, we can only provide the full length with cell free expression system and provide the partial protein with four regular expression systems.
Recombinant Human Stearoyl-CoA desaturase 5(SCD5)
CSB-CF020803HU
Expression Region: 1-330 aa; Full length.
Tag information:Tag type will be determined during the manufacturing process. (The expected tag is N-terminal 10xHis-tagged)
Sequence:
MPGPATDAGKIPFCDAKEEIRAGLESSEGGGGPERPGARGQRQNIVWRNVVLMSLLHLGAVYSLVLIPKAKPLTLLWAYFCFLLAALGVTAGAHRLWSHRSYRAKLPLRIFLAVANSMAFQNDIFEWSRDHRAHHKYSETDADPHNARRGFFFSHIGWLFVRKHRDVIEKGRKLDVTDLLADPVVRIQRKYYKISVVLMCFVVPTLVPWYIWGESLWNSYFLASILRYTISLNISWLVNSAAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKPMIEARKARTGDSSA
For the cell free expression system we can provide liposomal nanodiscs, but we can't guarantee to form a stable nanodiscs-PIP2-1 complex.We can try it, if it's failed, we will choose the detergent, and the detergent will be shown in the COA.
Usually, We will use detergent for the membrane proteins extraction, dissolving and purification. The final product is provided in a soluble form.
Partial protein expressed by the regular four expression systems:
Recombinant Human Stearoyl-CoA desaturase 5(SCD5),partial
CSB-YP020803HU1 >> Yeast
CSB-EP020803HU1 >> E.coli
CSB-BP020803HU1 >> Baculovirus
CSB-MP020803HU1 >> Mammalian cell
Expression Region: 239-330aa; Fragment at the C-terminal.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:
NSAAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPFDYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKPMIEARKARTGDSSA