Recombinant Mouse Mas-related G-protein coupled receptor member X1 (Mrgprx1)

Code CSB-CF817224MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Mrgprx1
Uniprot No.
Alternative Names
Mrgprx1; Mrgc11; Mrgprc11; Mas-related G-protein coupled receptor member X1; Mas-related G-protein coupled receptor member C11; Sensory neuron-specific G-protein coupled receptor 1
Species
Mus musculus (Mouse)
Expression Region
1-322
Target Protein Sequence
MDPTISSHDTESTPLNETGHPNCTPILTLSFLVLITTLVGLAGNTIVLWLLGFRMRRKAI SVYILNLALADSFFLCCHFIDSLLRIIDFYGLYAHKLSKDILGNAAIIPYISGLSILSAI STERCLCVLWPIWYHCHRPRNMSAIICALIWVLSFLMGILDWFSGFLGETHHHLWKNVDF IITAFLIFLFMLLSGSSLALLLRILCGPRRKPLSRLYVTIALTVMVYLICGLPLGLYLFL LYWFGVHLHYPFCHIYQVTAVLSCVNSSANPIIYFLVGSFRQHRKHRSLKRVLKRALEDT PEEDEYTDSHLHKTTEISESRY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Orphan receptor activated by neuropeptides terminating in Arg-Phe or Arg-Phe-amide. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. May regulate the function of nociceptive neurons by modulation of pain perception.
Gene References into Functions
  1. SLIGRL-amide inhibits influenza A virus infection independently of MRGPRC11 and independently of capsaicin-sensitive, neuropeptide-releasing sensory nerves. PMID: 27215903
  2. Der p1 also induced the release of IL-6 from heterologous cells expressing MRGPRX1. In summary, activation of Mrgprs by the allergen Der p1 may contribute to inflammation. PMID: 28768771
  3. Cathepsin S activates MrgprC11 and evokes receptor-dependent scratching in mice. PMID: 26216096
  4. Results suggest that activation of MrgC11 may inhibit high-voltage-activated calcium current in mouse dorsal root ganglion neurons through a voltage-independent mechanism that involves activation of the phospholipase C, but not Galphai or Galphas, pathway PMID: 26022362
  5. Mouse bone marrow-derived mast cells express the MrgC11 receptor agonist, RF-amide precursor gene proneuropeptide FF. PMID: 18250432

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family, Mas subfamily
Tissue Specificity
Expressed in a subset of IB4-positive small diameter nociceptive dorsal root neurons.
Database Links
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