Recombinant Mouse Membrane protein MLC1 (Mlc1)-VLPs

Code CSB-MP837739MO(A4)
Abbreviation Recombinant Mouse Mlc1 protein-VLPs
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Size $570
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  • CSB-MP837739MO(A4) is detected by Mouse anti-6*His monoclonal antibody
  • The purity of VLPs was greater than 90% as determined by SEC-HPLC
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Product Details

Purity
Greater than 90% as determined by SEC-HPLC.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Signal Transduction
Species
Mus musculus (Mouse)
Source
Mammalian cell
Expression Region
1-382aa
Target Protein Sequence
MTREGQFREELGYDRMPTLERGRQDAGRQDPGSYTPDSKPKDLQLSKRLPPCFSYKTWVFSVLMGSCLLVTSGFSLYLGNVFPSEMDYLRCAAGSCIPSAIVSFAVGRRNVSAIPNFQILFVSTFAVTTTCLIWFGCKLILNPSAININFNLILLLLLELLMAATVIISARSSEEPCKKKKGSISDGSNILDEVTFPARVLKSYSVVEVIAGVSAVLGGVIALNVEEAVSGPHLSVTFFWILVACFPSAIASHVTAECPSKCLVEVLIAISSLTSPLLFTASGYLSFSVMRVVEIFKDYPPAIKSYDVLLLLLLLLLLLQGGLNTGTAIQCVSFKVSARLQAASWDPQSCPQERPAGEVVRGPLKEFDKEKAWRAVVVQMAQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
43.0 kDa
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx.
Gene References into Functions
  1. Mlc1 overexpression reduced activity of the astrocytic sodium pump, which may underlie white matter edema followed by myelin membrane splitting PMID: 27748972
  2. This study demonistrated that Mlc1-null mice replicate the human with megalencephalic leukoencephalopathy with cysts. PMID: 25382142
  3. The complete cDNA sequence, exon-intron organization, and 5'-flanking regulatory region of murine Mlc1 have been reported. PMID: 12658361
  4. Because the temporal expression of Mlc1 indicates that, in contrast to developing and mature astrocytes, oligodendrocytes are devoid of Mlc1 expression, white matter tract abnormalities in these disorders may result from a primary astrocytic defect. PMID: 14603469
  5. Wkl1 is not associated with schizophrenia. PMID: 14755440
  6. Localization and functional analysis of Mlc1 were done in megalocephalic leukoencephalopathy with subcortical cysts. PMID: 15367490
  7. Our data provide a mouse MLC1 expression map that could be useful to understand the phenotype of MLC patients, and suggested that MLC disease is caused by an astrocytic and a neuronal dysfunction PMID: 17434314

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Subcellular Location
Membrane; Multi-pass membrane protein. Cell membrane. Cytoplasm, perinuclear region. Endoplasmic reticulum.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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