Recombinant Mouse Translocator protein(Tspo)-VLPs

Code CSB-MP025168MO
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Product Details

Target Names Tspo
Uniprot No. P50637
Research Area Cancer
Alternative Names Tspo; Bzrp; Mbr; Translocator protein; Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor; PBR
Species Mus musculus (Mouse)
Source Mammalian cell
Expression Region 1-169aa
Target Protein Sequence MPESWVPAVGLTLVPSLGGFMGAYFVRGEGLRWYASLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLALNWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLNYYVWRDNSGRRGGSRLPE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it’s possible to develop.
Form Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer Lyophilized from PBS, 6% Trehalose, pH 7.4
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis.
Gene References into Functions
  1. induced TSPO expression marks a pro-inflammatory brain environment that is not necessarily accompanied by neuronal loss. PMID: 28695372
  2. tertiary and quaternary structure of the mitochondrial translocator protein TSPO, which binds cholesterol with nanomolar affinity, is affected by cholesterol. PMID: 28358007
  3. Steroidogenic abnormalities in TSPO-knockout mice and significance in the aging male have been reported. PMID: 29127254
  4. Results indicated that TSPO overexpression in the hippocampal dentate gyrus could significantly exert anxiolytic and antidepressant-like effects on behaviour. These effects were associated with allopregnanolone biosynthesis, which was at least partially mediated by TSPO. PMID: 28655607
  5. TSPO is therefore proposed as a novel outer mitochondrial membrane-based pathway to control intracellular Ca(2+) dynamics and redox transients in neuronal cytotoxicity. PMID: 28640253
  6. Critical role of TSPO in steroid biosynthesis and it may function at least in part via its regulation of DeltaPsim and effects on STAR. PMID: 29300865
  7. Results showed that TSPO and other mitophagy related proteins, such as VDAC1, Pink1 and Beclin1 were significantly decreased by LH challenge. Moreover, KIFC2, relevant to the mitochondrial transport and Snap25, relevant to neurotransmitter vesicle release, were also obviously down-regulated in the LH mice, which further rendered supportive evidence for the existing mitochondrial dysfunction in LH mice. PMID: 27543827
  8. A potential modulatory role of TSPO is in response to immune system deficit. PMID: 28189343
  9. The expression of TSPO was found to be increased in the peri-hematomal brain region after intracerebral hemorrhage. PMID: 27315802
  10. TSPO expression increases ( approximately 9-fold) in rodent-derived macrophages and microglia upon pro-inflammatory stimulation. PMID: 28530125
  11. Data suggest that up-regulation of translocator protein 18kDa level during neuroinflammation may be an adaptive response mechanism, a way to provide more neurosteroids. PMID: 27265418
  12. This study confirms TSPO deficiency does not affect viability and bronchial alveolar immune homeostasis. PMID: 27907096
  13. These results suggested that Wuling powder exhibited an obvious antidepressant effect, which could be due to the improvement of TSPO-mediated mitophagy signaling pathway. PMID: 27063986
  14. The loss of TSPO in the Central Nervous System did not result in overt developmental defects or phenotypes. The TSPO-/- mouse showed a decrease in glial fibrillary acidic protein expression, correlating with a decrease in astrogliosis in response to neural injury during Autoimmune Experimental Encephalomyelitis. PMID: 26925573
  15. Study reviews the physiological functions of TSPO gene in the mitochondrial permeability transition pore, steroidogenesis and energy metabolism especially in knockout mice. [review] PMID: 26551692
  16. Study highlights the 3D structure of mTSPO/PBR and in complex with PK11195 providing an important step towards a more detailed understanding of the molecular mechanism of mammalian TSPO/PBR and its interaction with small molecules. [review] PMID: 26551694
  17. TSPO expression in Sandhoff disease mice is brain region-specific and depends on the age of the animal as disease progresses with the thalamus providing the earliest signal of disease based on TSPO expression and other markers of neurodegeneration PMID: 26545928
  18. TSPO deficiency does not adversely affect erythropoiesis, heme biosynthesis, bioconversion of ALA to PPIX, and porphyrin-mediated phototoxic cell death. PMID: 26627829
  19. TSPO can affect mitochondrial energy homeostasis through modulation of fatty acid oxidation, a function that appears to be consistent with high levels of TSPO expression observed in cell types active in lipid storage/metabolism. PMID: 26741196
  20. Determined is the three-dimensional structure and side-chain dynamics of the A147T polymorph of mouse TSPO in complex with the first-generation ligand PK11195. PMID: 25974690
  21. Report novel imidazo[1,2-a]pyridine-based TSPO ligands endowed with potential in modulating the steroidogenesis process in murine Leydig tumor cells. PMID: 26002041
  22. TSPO as a novel element in the regulation of mitochondrial quality control by autophagy, and demonstrate the importance for cell homeostasis of its expression ratio with voltage-dependent anion channel 1 PMID: 25470454
  23. TPSO knockout mice have normal growth, lifespan, cholesterol transport, blood pregnenolone level, protoporphyrin IX metabolism, fertility and behavior. Activation of microglia after neuronal injury appears unimpaired, but microglia produce less ATP. PMID: 25406832
  24. Data suggest that Tspo expression is tightly associated with metabolic state of adipocytes and differentiation of preadipocytes into white/brown adipocytes; Tspo expression may improve metabolic status of adipocytes and regulate glucose homeostasis. PMID: 26123521
  25. TSPO was found necessary for preimplantation embryo development and ACTH-stimulated steroid biosynthesis PMID: 26039990
  26. This study reported on generation of TSPO-knockout MA-10 mouse Leydig tumor cells and examined their steroidogenic potential after exposure to either dibutyryl-cAMP or PK11195. PMID: 25535830
  27. Data indicate that knockdown of translocator protein TSPO by siRNAhad showed no effect on the productions of TNF-alpha, IL-1beta and IL-6 in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells. PMID: 25200148
  28. Up-regulation of TSPO in microglia occurs during neuroinflammation. PMID: 24687172
  29. TSPO in a number of pathological processes through its actions on the PTP PMID: 24692541
  30. TSPO knock-out mice are viable with no effects on steroidogenesis. PMID: 24936060
  31. TSPO expression is able to distinguish differences in the severity of myocarditis between male subjects and female subjects. PMID: 24402571
  32. A reduction in TSPO gene expression in whole tissue extracts. PMID: 24260329
  33. TSPO expression in both male and female reproductive tissues is not only restricted to steroidogenic cells. PMID: 24040265
  34. 3D structure of TSPO in complex with its ligand PK11195; TSPO-PK11195 structure is described by a tight bundle of 5 transmembrane alpha helices that form a hydrophobic pocket accepting PK11195; ligand-induced stabilization of TSPO structure suggests a molecular mechanism for stimulation of cholesterol transport into mitochondria PMID: 24653034
  35. TSPO function is not essential for steroid hormone biosynthesis PMID: 24174323
  36. Upregulation of TSPO expression is increased during microglial inflammation. PMID: 22874716
  37. analysis of central nervous system expression and PET imaging of the translocator protein in relapsing-remitting experimental autoimmune encephalomyelitis PMID: 23321458
  38. different amino acid compositions and the location of the polyhistidine tag between bacterial and mouse TSPO could account for the formation of dimer versus tetramer, respectively PMID: 22771765
  39. The results suggested a new micro-transcriptional mechanism that regulates Tspo expression and thus steroidogenesis via an intron-based SINE B2-driven natural antisense transcript specific for the Tspo gene. PMID: 22378763
  40. the expression of TSPO in small rodent bone tissues PMID: 22295097
  41. Translocator protein (TSPO) expression is upregulated in cerebral regions of ob/ob leptin-deficient mice. PMID: 21299850
  42. Novel androstenetriol interacts with the mitochondrial translocator protein and controls steroidogenesis. PMID: 21209087
  43. Clinical consequences of secondary neuronal degeneration in stroke might be better treated thanks to the discrimination of neuronal processes using in vivo molecular imaging and potent TSPO radioligands like CLINDE to guide therapeutic interventions. PMID: 20814674
  44. In conclusion, [(11)C]DAC PET responded to the change in PBR expression in tumors caused by carbon ion irradiation in this study. PMID: 19851043
  45. The capacity of PK11195 to suppress the pathogenesis of microphthalmia implies a critical role for mitochondrial peripheral benzodiazepine receptors in the p53-dependent mode of action of 2CdA on ocular development. PMID: 12769506
  46. two Sp1/Sp3 sites in the proximal promoter are important for basal activity in all cell lines tested and steroidogenic MA-10 and Y1 cells use different areas of the promoter compared with nonsteroidogenic NIH-3T3 cells. PMID: 14630713
  47. PBR deregulation has role in human malignancy [isoquinoline-binding protein] PMID: 15379570
  48. both StAR and PBR proteins are indispensable elements of the steroidogenic machinery and function in a coordinated manner to transfer cholesterol into mitochondria PMID: 15498831
  49. the CRAC domain in the cytosolic carboxyl-terminal domain of PBR might be responsible for the uptake and translocation of cholesterol into the mitochondria PMID: 15528269
  50. study is the first to show that peripheral benzodiazepine receptor and DRM/gremlin are expressed in preadipocyte cell lines and that they are differentially regulated during adipogenesis PMID: 15919833

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Subcellular Location Mitochondrion membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Protein Families TspO/BZRP family
Tissue Specificity Detected in liver (at protein level). Ubiquitous.
Database Links

KEGG: mmu:12257

STRING: 10090.ENSMUSP00000037039

UniGene: Mm.1508

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