Recombinant Rat Long-chain-fatty-acid--CoA ligase 1(Acsl1)

Code CSB-CF001191RA
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Acsl1
Uniprot No. P18163
Alternative Names Acsl1; Acs1; Acsl2; Facl2; Long-chain-fatty-acid--CoA ligase 1; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 1; LACS 1; Long-chain-fatty-acid--CoA ligase, liver isozyme; Phytanate--CoA ligase
Species Rattus norvegicus (Rat)
Expression Region 1-699
Target Protein Sequence MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).
Gene References into Functions
  1. here we revealed that ACSs (Faa1 and Faa4 in yeast and ACSL1, ACSL4, ACSL5, ACSL6, and ACSVL4 in mammals) are involved in LCB uptake. In addition, we provided important insight into the molecular mechanism of LCFA transport PMID: 27136724
  2. Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function PMID: 26136511
  3. Acsl isozymes play distinct roles in the control of AA remodeling in rat fibroblasts: Acsl4 acts as the first step of enzyme for AA remodeling following IL-1beta stimulation, and Acsl1 is involved in the maintenance of some AA-containing PC species. PMID: 24095834
  4. The fatty metabolism is stimulated in the CNS during a chronic pain state. PMID: 14622223
  5. Acyl-CoA synthetase 2 has a role in fatty acid internalization and neurite outgrowth PMID: 15051725
  6. Long-chain acyl-CoenzymeA synthetase-2 expression is significantly upregulated in distinct regions of the frontal cortex in learned helpless rats relative to controls PMID: 15093965
  7. rat long chain acyl-coa synthetase 1 has a role in fatty acid metabolism in rat primary hepatocytes PMID: 17028193

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Subcellular Location Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.
Protein Families ATP-dependent AMP-binding enzyme family
Tissue Specificity Liver, heart, epididymal adipose and to a lesser extent brain, small intestine and lung.
Database Links

KEGG: rno:25288

STRING: 10116.ENSRNOP00000014235

UniGene: Rn.6215

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