Recombinant Rat Neuromedin-U receptor 2 (Nmur2)

Code CSB-CF875367RA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Nmur2
Uniprot No.
Alternative Names
Nmur2; Tgr1; Neuromedin-U receptor 2; NMU-R2; G-protein coupled receptor TGR-1; G-protein-coupled receptor FM-4
Species
Rattus norvegicus (Rat)
Expression Region
1-395
Target Protein Sequence
MGKLENASWIHDPLMKYLNSTEEYLAHLCGPKRSDLSLPVSVAYALIFLVGVMGNLLVCM VIVRHQTLKTPTNYYLFSLAVSDLLVLLLGMPLEIYEMWHNYPFLFGPVGCYFKTALFET VCFASILSVTTVSVERYVAIVHPFRAKLESTRRRALRILSLVWSFSVVFSLPNTSIHGIK FQHFPNGSSVPGSATCTVTKPMWVYNLIIQATSFLFYILPMTLISVLYYLMGLRLKRDES LEANKVAVNIHRPSRKSVTKMLFVLVLVFAICWTPFHVDRLFFSFVEEWTESLAAVFNLI HVVSGVFFYLSSAVNPIIYNLLSRRFRAAFRNVVSPTCKWCHPRHRPQGPPAQKIIFLTE CHLVELTEDAGPQFPGQSSIHNTNLTTAPCAGEVP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for the neuromedin-U and neuromedin-S neuropeptides.
Gene References into Functions
  1. Results demonstrate that NMUR2 signaling in the paraventricular nucleus of the hypothalamus regulates consumption and preference for high-fat foods without disrupting feeding behavior associated with non-obesogenic standard chow PMID: 24269937
  2. Results show that central-activation of neuromedin U2 receptor markedly reduced sleep duration and disrupted the mechanisms underlying NREM-REM sleep transitions. PMID: 21296417
  3. These results suggest that NMU modulates the function of the PVN putative parvocellular neurons and is involved in the regulation of OTergic and VPergic neurons by enhanced HCN ion channels activity via NMU-receptor 2. PMID: 15631899
  4. Expression of NMS and NMU2R genes was detected at the hypothalamus along postnatal development, with significant fluctuations of their relative levels (maximum at prepubertal stage and adulthood). PMID: 17110433
  5. endogenous rat NmU receptors couple to both Galpha(q/11) and Galpha(i) G-proteins. Challenge of either circular or longitudinal rat isolated colonic smooth muscle. preparations with NmU resulted in robust contractions PMID: 18180374
  6. cultured rat calvarial osteoblast-like cells are provided with NMUR2, the receptor isoform typical for the central nervous system PMID: 18698496

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
The highest level is detected in the uterus. In the central nervous system, high expression levels were found in the hypothalamus and moderate levels in both the medulla oblongata and spinal cord. Expressed in the hypothalamic paraventricular nucleus (PVN
Database Links
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