| Code |
CSB-E04639m |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
P08505
|
| Lead Time |
3-5 working days |
| Abbreviation |
IL6 |
| Protein Biological Process 1 |
Immunity |
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alias |
BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2 |
| Species |
Mus musculus (Mouse) |
| Protein Biological Process 3 |
Acute phase |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Target Details |
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
91 |
|
| Range % |
87-95 |
|
| 1:2 |
Average % |
93 |
|
| Range % |
85-99 |
|
| 1:4 |
Average % |
95 |
|
| Range % |
90-102 |
|
| 1:8 |
Average % |
87 |
|
| Range % |
82-92 |
|
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| Recovery |
| The recovery of mouse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
85-97 |
|
| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.820 |
2.789 |
2.805 |
2.623 |
|
| 50 |
2.307 |
2.238 |
2.273 |
2.091 |
|
| 25 |
1.558 |
1.537 |
1.548 |
1.366 |
|
| 12.5 |
0.800 |
0.763 |
0.782 |
0.600 |
|
| 6.25 |
0.515 |
0.488 |
0.502 |
0.320 |
|
| 3.12 |
0.357 |
0.341 |
0.349 |
0.167 |
|
| 1.56 |
0.287 |
0.266 |
0.277 |
0.095 |
|
| 0 |
0.184 |
0.180 |
0.182 |
|
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Mice lacking myotubularin-related protein 14 show accelerated high-fat diet-induced lipid accumulation and inflammation. Yin L.et al,Journal of Physiology and Biochemistry,Jan 12
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L-F001, a novel multifunctional ROCK inhibitor, suppresses neuroinflammation in vitro and in vivo: Involvement of NF-kB inhibition and Nrf2 pathway activation Chen J.et al,European Journal of Pharmacology,Mar 16.
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