Mouse Interleukin 6,IL-6 ELISA KIT

Instructions
Code CSB-E04639m
Size 96T,5×96T,10×96T

Product Details

Target Name interleukin 6 (interferon, beta 2)
Alternative Names BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2
Abbreviation IL6
Uniprot No. P08505
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 87-95  
1:2 Average % 93  
Range % 85-99  
1:4 Average % 95  
Range % 90-102  
1:8 Average % 87  
Range % 82-92  
Recovery
The recovery of mouse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 85-97  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.820 2.789 2.805 2.623  
50 2.307 2.238 2.273 2.091  
25 1.558 1.537 1.548 1.366  
12.5 0.800 0.763 0.782 0.600  
6.25 0.515 0.488 0.502 0.320  
3.12 0.357 0.341 0.349 0.167  
1.56 0.287 0.266 0.277 0.095  
0 0.184 0.180 0.182    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Serum
    Sample species: Mouse
    Review: In addition, TNF-α and IL-6 levels were increased in the ethanol-fed mice, and CR2-Crry treatment significantly reduced the levels of both cytokines.
    PMID: 31076642
  • Sample type: Serum
    Sample species: Mouse
    Review: Serum proinflammatory cytokines levels including interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and interferon gamma (IFN-γ) were measured after two-cycle treatments.
    PMID: 30886812
  • Sample type: Supernatants
    Sample species: Mouse
    Review: BV2 cells (1 × 105 cells per well in a 24-well plate) were pretreated with treatments for 1 h and stimulated with LPS (100 ng mL−1). After treatment for 24 h and 48 h, the supernatants were collected. The concentrations of TNF-α, IL-1β, IL-6, IL-12, and iNOS in the culture medium were measured by ELISA kits.
    PMID: 30962431
  • Sample type: Plasma
    Sample species: Mouse
    Review: The results of ELISA assays indicated that the expression levels of common proinflammatory indicators, such as IL-6 and IL-8, were significantly higher in the OA group compared to those in the normal group.
    PMID: 29774080
  • Sample type: Plasma
    Sample species: Mouse
    Review: ELISA was used to analyse the levels of inflammation factors after renal I/R injury.
    PMID: 30384260
  • Sample type: Pleural flui
    Sample species: Mice
    Review: The presence of interferon-γ, IL-2, TNF-α, monocyte chemotactic protein (MCP) -1, and IL-6 in pleural tissue was quantified using an enzyme-linked immunosorbent assay. The concentration of cytokines (IL-6, TNFA, IFN, MCP-1, IL-2) in pleural effusion were higher in mice in the BCG+ SiCon group than in the PBS+ SiCon group..
    PMID: 30669315
  • Sample type: Supernatant
    Sample species: Mice
    Review: Levels of TNF-α (CSB-E04741m), IL-6 (CSB-E04639m) and PGE2 (CSB-E07966m) in supernatant of colorectal mucosa isolated from mice were quantified by using ELISA kits according to the manufacturer's protocols. ELISA analysis of IL-6, TNF-α and PGE2 in the intraepithelial and lamina propria showed significant differences between WT and Igf1r+/- mice.
    PMID: 30611867
  • Sample type: Plasma
    Sample species: Mouse
    Review: IL-6 concentrations in mouse plasma as well as in ex vivo culture solution were measured using a mouse IL-6 ELISA kit (CSB-E04639m) according to the manufacturer’s instructions.Plasma IL-6 concentration (n=8 per group). Data are presented as the mean±SD or as median and IQR (for elastin degradation score). *P<0.05, **P<0.01.
    PMID: 30991034

Target Data

Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: mmu:16193

STRING: 10090.ENSMUSP00000026845

UniGene: Mm.1019

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