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| Code | CSB-E04639m |
| Size | 96T,5×96T,10×96T |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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The mouse IL-6 ELISA kit (CSB-E04639m) is designed for the quantitative measurement of mouse IL-6 protein in serum, plasma, or cell culture supernates. It quantitates mouse IL-6 with 0.39 pg/ml sensitivity and shows excellent specificity for mouse IL-6. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated IL-6 antibody that recognizes the analyte bound by the immobilized IL-6 antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of IL-6 in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse IL-6 in the sample. This mouse IL-6 ELISA kit has been cited in up to 154 publications.
IL-6 is a pleiotropic cytokine generated in response to tissue injury and infections. IL-6 binds to IL-6R and then associates with gp130, which dimerizes and triggers multiple intracellular signaling pathways, including JAK/STAT1/3, YES/YAP, PI3K/AKT, and RAS/MAPK pathways that finally induce pro- or anti-inflammation. IL-6 is involved in immune responses and inflammation, hematopoiesis, bone metabolism, and embryonic development. Deregulation of IL-6 is linked to chronic inflammation and multifactorial auto-immune disorders. Targeting IL-6 shows potential in the diagnosis, management, and prevention of infectious diseases in the clinical.
Sample type: Serum
Sample species: Mouse
Review: In addition, TNF-α and IL-6 levels were increased in the ethanol-fed mice, and CR2-Crry treatment significantly reduced the levels of both cytokines.
By Anonymous
Sample type: Serum
Sample species: Mouse
Review: Serum proinflammatory cytokines levels including interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and interferon gamma (IFN-γ) were measured after two-cycle treatments.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Review: BV2 cells (1 × 105 cells per well in a 24-well plate) were pretreated with treatments for 1 h and stimulated with LPS (100 ng mL−1). After treatment for 24 h and 48 h, the supernatants were collected. The concentrations of TNF-α, IL-1β, IL-6, IL-12, and iNOS in the culture medium were measured by ELISA kits.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Review: Levels of TNF-α (CSB-E04741m), IL-6 (CSB-E04639m) and PGE2 (CSB-E07966m) in supernatant of colorectal mucosa isolated from mice were quantified by using ELISA kits according to the manufacturer\'s protocols. ELISA analysis of IL-6, TNF-α and PGE2 in the intraepithelial and lamina propria showed significant differences between WT and Igf1r+/- mice.
By Anonymous
Sample type: Biological fluids(Pleural fluid)
Sample species: Mouse
Review: The presence of interferon-γ, IL-2, TNF-α, monocyte chemotactic protein (MCP) -1, and IL-6 in pleural tissue was quantified using an enzyme-linked immunosorbent assay. The concentration of cytokines (IL-6, TNFA, IFN, MCP-1, IL-2) in pleural effusion was higher in mice in the BCG+ SiCon group than in the PBS+ SiCon group.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: The results of ELISA assays indicated that the expression levels of common proinflammatory indicators, such as IL-6 and IL-8, were significantly higher in the OA group compared to those in the normal group.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: ELISA was used to analyze the levels of inflammation factors after renal I/R injury.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: IL-6 concentrations in mouse plasma as well as in ex vivo culture solution were measured using a mouse IL-6 ELISA kit (CSB-E04639m) according to the manufacturer’s instructions. Plasma IL-6 concentration (n=8 per group). Data are presented as the mean±SD or as median and IQR (for elastin degradation score). *P<0.05, **P<0.01.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Sample dilution: No dilution
Review: I used CSB-E04639m to detect IL6 levels in cell supernatant exosomes and mouse supernatants, respectively. The results of the standard curve were good, as shown in the Appendix. However, during the actual assay, probably due to the small changes in IL-6 in the treated group, the majority of values were below the detection limit, but some values could still be detected after increasing the sample size, and the final results could be used in the article.
By Anonymous
