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| Code | CSB-E04639m |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The mouse IL-6 ELISA kit (CSB-E04639m) is designed for the quantitative measurement of mouse IL-6 protein in serum, plasma, or cell culture supernates. It quantitates mouse IL-6 with 0.39 pg/ml sensitivity and shows excellent specificity for mouse IL-6. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated IL-6 antibody that recognizes the analyte bound by the immobilized IL-6 antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of IL-6 in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse IL-6 in the sample. This mouse IL-6 ELISA kit has been cited in up to 154 publications. IL-6 is a pleiotropic cytokine generated in response to tissue injury and infections. IL-6 binds to IL-6R and then associates with gp130, which dimerizes and triggers multiple intracellular signaling pathways, including JAK/STAT1/3, YES/YAP, PI3K/AKT, and RAS/MAPK pathways that finally induce pro- or anti-inflammation. IL-6 is involved in immune responses and inflammation, hematopoiesis, bone metabolism, and embryonic development. Deregulation of IL-6 is linked to chronic inflammation and multifactorial auto-immune disorders. Targeting IL-6 shows potential in the diagnosis, management, and prevention of infectious diseases in the clinical. |
| Alternative Names | Il6 ELISA Kit; Il-6Interleukin-6 ELISA Kit; IL-6 ELISA Kit; B-cell hybridoma growth factor ELISA Kit; Interleukin HP-1 ELISA Kit |
| Abbreviation | IL6 |
| Uniprot No. | P08505 |
| Species | Mus musculus (Mouse) |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 1.56 pg/ml-100 pg/ml. |
| Sensitivity | 0.39 pg/ml. |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
Sample type: Serum
Sample species: Mouse
Review: In addition, TNF-α and IL-6 levels were increased in the ethanol-fed mice, and CR2-Crry treatment significantly reduced the levels of both cytokines.
By Anonymous
Sample type: Serum
Sample species: Mouse
Review: Serum proinflammatory cytokines levels including interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and interferon gamma (IFN-γ) were measured after two-cycle treatments.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Review: BV2 cells (1 × 105 cells per well in a 24-well plate) were pretreated with treatments for 1 h and stimulated with LPS (100 ng mL−1). After treatment for 24 h and 48 h, the supernatants were collected. The concentrations of TNF-α, IL-1β, IL-6, IL-12, and iNOS in the culture medium were measured by ELISA kits.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Review: Levels of TNF-α (CSB-E04741m), IL-6 (CSB-E04639m) and PGE2 (CSB-E07966m) in supernatant of colorectal mucosa isolated from mice were quantified by using ELISA kits according to the manufacturer\'s protocols. ELISA analysis of IL-6, TNF-α and PGE2 in the intraepithelial and lamina propria showed significant differences between WT and Igf1r+/- mice.
By Anonymous
Sample type: Biological fluids(Pleural fluid)
Sample species: Mouse
Review: The presence of interferon-γ, IL-2, TNF-α, monocyte chemotactic protein (MCP) -1, and IL-6 in pleural tissue was quantified using an enzyme-linked immunosorbent assay. The concentration of cytokines (IL-6, TNFA, IFN, MCP-1, IL-2) in pleural effusion was higher in mice in the BCG+ SiCon group than in the PBS+ SiCon group.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: The results of ELISA assays indicated that the expression levels of common proinflammatory indicators, such as IL-6 and IL-8, were significantly higher in the OA group compared to those in the normal group.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: ELISA was used to analyze the levels of inflammation factors after renal I/R injury.
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Mouse
Review: IL-6 concentrations in mouse plasma as well as in ex vivo culture solution were measured using a mouse IL-6 ELISA kit (CSB-E04639m) according to the manufacturer’s instructions. Plasma IL-6 concentration (n=8 per group). Data are presented as the mean±SD or as median and IQR (for elastin degradation score). *P<0.05, **P<0.01.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Sample dilution: No dilution
Review: I used CSB-E04639m to detect IL6 levels in cell supernatant exosomes and mouse supernatants, respectively. The results of the standard curve were good, as shown in the Appendix. However, during the actual assay, probably due to the small changes in IL-6 in the treated group, the majority of values were below the detection limit, but some values could still be detected after increasing the sample size, and the final results could be used in the article.
By Anonymous
| Function (From Uniprot) |
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
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| Gene References into Functions |
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| Subcellular Location | Secreted. |
| Protein Families | IL-6 superfamily |
| Tissue Specificity | Expressed by dendritic cells and macrophages. Expressed by activated follicular B cells. Abundantly expressed in the central nervous system (CNS), particularly the hypothalamic region. |
| Database Links |
KEGG: mmu:16193 STRING: 10090.ENSMUSP00000026845 UniGene: Mm.1019 |
