Mouse Interleukin 6,IL-6 ELISA KIT

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Mouse Interleukin 6,IL-6 ELISA KIT

Name: Mouse Interleukin 6,IL-6 ELISA KIT
Brand: CUSABIO
SKU: CSB-E04639m

^Instructions

Code	CSB-E04639m
Size	96T,5×96T,10×96T

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Target Data

Target Name

interleukin 6 (interferon, beta 2)

Alternative Names

Abbreviation

IL6

Uniprot No.

P08505

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

0.39 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-95
1:2	Average %	93
	Range %	85-99
1:4	Average %	95
	Range %	90-102
1:8	Average %	87
	Range %	82-92

Recovery

The recovery of mouse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-97
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.820	2.789	2.805	2.623
50	2.307	2.238	2.273	2.091
25	1.558	1.537	1.548	1.366
12.5	0.800	0.763	0.782	0.600
6.25	0.515	0.488	0.502	0.320
3.12	0.357	0.341	0.349	0.167
1.56	0.287	0.266	0.277	0.095
0	0.184	0.180	0.182

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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■ Customer Reviews

Sample type: Serum
Sample species: Mouse
Review: In addition, TNF-α and IL-6 levels were increased in the ethanol-fed mice, and CR2-Crry treatment significantly reduced the levels of both cytokines.
PMID: 31076642
Sample type: Serum
Sample species: Mouse
Review: Serum proinflammatory cytokines levels including interleukin 2 (IL-2), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and interferon gamma (IFN-γ) were measured after two-cycle treatments.
PMID: 30886812
Sample type: Supernatants
Sample species: Mouse
Review: BV2 cells (1 × 10⁵ cells per well in a 24-well plate) were pretreated with treatments for 1 h and stimulated with LPS (100 ng mL⁻¹). After treatment for 24 h and 48 h, the supernatants were collected. The concentrations of TNF-α, IL-1β, IL-6, IL-12, and iNOS in the culture medium were measured by ELISA kits.
PMID: 30962431
Sample type: Plasma
Sample species: Mouse
Review: The results of ELISA assays indicated that the expression levels of common proinflammatory indicators, such as IL-6 and IL-8, were significantly higher in the OA group compared to those in the normal group.
PMID: 29774080
Sample type: Plasma
Sample species: Mouse
Review: ELISA was used to analyse the levels of inflammation factors after renal I/R injury.
PMID: 30384260
Sample type: Pleural flui
Sample species: Mice
Review: The presence of interferon-γ, IL-2, TNF-α, monocyte chemotactic protein (MCP) -1, and IL-6 in pleural tissue was quantified using an enzyme-linked immunosorbent assay. The concentration of cytokines (IL-6, TNFA, IFN, MCP-1, IL-2) in pleural effusion were higher in mice in the BCG+ SiCon group than in the PBS+ SiCon group..
PMID: 30669315
Sample type: Supernatant
Sample species: Mice
Review: Levels of TNF-α (CSB-E04741m), IL-6 (CSB-E04639m) and PGE2 (CSB-E07966m) in supernatant of colorectal mucosa isolated from mice were quantified by using ELISA kits according to the manufacturer's protocols. ELISA analysis of IL-6, TNF-α and PGE2 in the intraepithelial and lamina propria showed significant differences between WT and Igf1r+/- mice.
PMID: 30611867
Sample type: Plasma
Sample species: Mouse
Review: IL-6 concentrations in mouse plasma as well as in ex vivo culture solution were measured using a mouse IL-6 ELISA kit (CSB-E04639m) according to the manufacturer’s instructions.Plasma IL-6 concentration (n=8 per group). Data are presented as the mean±SD or as median and IQR (for elastin degradation score). *P<0.05, **P<0.01.
PMID: 30991034

Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: mmu:16193 STRING: 10090.ENSMUSP00000026845 UniGene: Mm.1019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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