Mouse Klotho ELISA Kit

Instructions
Code CSB-E14362m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name klotho
Alternative Names Kl ELISA Kit; Klotho ELISA Kit; EC 3.2.1.31) [Cleaved into: Klotho peptide] ELISA Kit
Abbreviation KL
Uniprot No. O35082
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-90  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 92  
Range % 87-99  
1:8 Average % 95  
Range % 90-102  
Recovery
The recovery of mouse klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-104  
EDTA plasma (n=4) 98 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.157 2.107 2.132 1.968  
100 1.372 1.334 1.353 1.189  
50 0.805 0.798 0.802 0.638  
25 0.516 0.504 0.510 0.346  
12.5 0.348 0.331 0.340 0.176  
6.25 0.273 0.258 0.266 0.102  
3.12 0.228 0.215 0.222 0.058  
0 0.168 0.160 0.164    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; FUNCTION
Gene References into Functions
  1. The present study analyzed the gene expression profile in salivary glands from accelerated aging klotho deficient mice. PMID: 28885690
  2. The studies identify a role for alpha-Klotho in the regulation of muscle progenitor cell mitochondrial function and implicate alpha-Klotho declines as a driver of impaired muscle regeneration with age. PMID: 30451844
  3. our study identified Klotho loss as a key event linking HDAC deregulation to the renal and bone injuries in CKD-MBD mice and demonstrated the therapeutic potentials of endogenous Klotho restoration by HDAC inhibition in treating CKD and the associated extrarenal complications. PMID: 28387374
  4. Klotho has posed antioxidant and anti-apoptotic effects on oxidative injuries in TCMK-1 cells, which might be partially related to its inhibition of JNK/MAPK and p38/MAPK phosphorylation and subsequent elevation of antioxidant enzymes. Increasing Klotho expression has played a protective role against oxidative stress in tubular epithelial cells. PMID: 29285593
  5. Klotho expression in the central nervous system is associated with total antioxidant capacity in mice with experimental autoimmune encephalomyelitis. PMID: 29542092
  6. Using mouse strains with parathyroid-specific deletions of Klotho and CaSR and dual deletion of both genes, Klotho has a pivotal role in suppressing PTH in the absence of CaSR. Chronic hypocalcemia and ex vivo PTG culture demonstrated an independent role for Klotho in mediating PTH secretion. PMID: 29618612
  7. DPP-4 inhibition with linagliptin slowed the progression of premature aging in klotho-/- mice. Provide a novel insight into the potential role of DPP-4 in the mechanism of premature aging. PMID: 29195509
  8. Klotho protects tubular epithelial cells from renal ischemic-reperfusion injury and its anti-necroptotic role may be associated with oxidative stress inhibition. PMID: 29550818
  9. studies uncover a novel hypomethylating character of Rhein in preventing Klotho loss and renal fibrosis, and demonstrate the efficacy of Klotho-targeted epigenetic intervention in potential treatment of renal fibrosis-associated kidney diseases. PMID: 27703201
  10. the main physiological function of Klotho for mineral homeostasis in vivo is its role as co-receptor mediating Fgf23 action. PMID: 28600880
  11. Findings establish a key role of osteocyte-expressed Klotho in regulating bone metabolism and indicate a new mechanism by which osteocytes control bone formation. PMID: 28396120
  12. Selective HDAC3 inhibition effectively alleviated Klotho loss and kidney injury, whereas the protective effects were largely abolished when Klotho was knocked down by siRNA, suggesting that aberrant HDAC3 and Klotho loss are crucial components involved in the renal damage of mice with chronic kidney disease. PMID: 28416226
  13. data showed that Klotho protects Tac-induced oxidative stress by negatively regulating the PI3K/AKT pathway and subsequently enhancing FoxO3a-mediated MnSOD expression. PMID: 28771227
  14. The present study provides a novel approach for klotho gene therapy and demonstrates that direct up-regulation of klotho in the brain might improve aging-related memory impairments and decrease oxidative stress. PMID: 29544758
  15. Klotho inhibits cigarette smoke-induced autophagy via down-regulation of IGF-1R, Akt, and ERK phosphorylation in alveolar macrophages. PMID: 28349330
  16. In conclusion, the excessive 1,25(OH)2D3 formation in klotho-hypomorphic mice has a profound effect on murine behavior. PMID: 27109615
  17. Klotho is a novel regulator of postnatal neurogenesis affecting neural stem cell proliferation and maturation sufficient to impact hippocampal-dependent spatial memory function. PMID: 28837861
  18. Reduced Klotho expression aggravates tacrolimus-induced renal injury via the PI3K-Akt-FoxO pathway. PMID: 27174564
  19. Two distinct isoforms of soluble Klotho appear to play counter-regulatory roles in cardiac fibrogenic responses. PMID: 27543985
  20. Klotho regulates adipogenic differentiation in adipose-derived stem cells, likely via inhibition of TGFbeta1 and activation of PPAR-gamma. PMID: 26865060
  21. Review of Klotho function and dysfunction in mice and humans. [Review Article] PMID: 27576165
  22. Overexpression of Klotho inhibits the differentiation of RAW264.7 cells into osteoclasts. PMID: 28615093
  23. Data suggest that klotho affects cognition, at least in part, by regulating hippocampal synaptic plasticity. PMID: 28215989
  24. Adiponectin alters calcium and phosphate balance and renal mineral excretion, in part, through klotho. PMID: 27914707
  25. These results support the notion that sialogangliosides and lipid rafts are membrane receptors for sKlotho and that the KL1 domain is sufficient for the tested biologic activities. PMID: 28442546
  26. Klotho in bone is crucial for inducing FGF23 production upon renal failure PMID: 28183805
  27. Results are the first to suggest an integrated regulatory function of Klotho in the distal and proximal tubules on mineral metabolism. PMID: 27292223
  28. Data show that Klotho protein is present in the heart, and reduced levels of myocardial Klotho in aging mice is accompanied by lower levels heat shock protein 70 (HSP70) in the myocardium. PMID: 28152512
  29. Klotho preserves AEC mtDNA integrity in the setting of oxidative stress necessary for preventing apoptosis and asbestos-induced lung fibrosis. PMID: 28428174
  30. epigenetic silencing of klotho during muscular dystrophy contributes substantially to lost regenerative capacity and increased fibrosis of dystrophic muscle during late progressive stages of the disease. PMID: 27154199
  31. Klotho gene deficiency promotes high-fat diet-induced fibrosis in aortic valves, likely through the AMPKalpha-RUNX2 pathway. PMID: 27242197
  32. Klotho can modulate inflammation via PDLIM2/NF-kappaB p65 pathway in cyclosporine A-induced nephropathy. PMID: 28315683
  33. the histological malformation in periodontal tissues in alphaKlotho-deficient mice appears to be due to not only increased concentration of inorganic phosphate but also disrupted alphaklotho/FGF23 signaling PMID: 28122194
  34. Results show that Klotho regulates adrenal CYP11B2 expression. Klotho deficiency-induced spontaneous hypertension and kidney damage may be partially attributed to the upregulation of CYP11B2 expression and aldosterone synthesis. PMID: 26471128
  35. Aberrant Klotho expression contributes to systemic chronic inflammation in Klotho-/- mice. PMID: 28259911
  36. Thus, higher levels of FGF23 in kl/kl mouse may have a role to increase TNF-alpha production in would lesion independently of alpha-Klotho protein, and impair granulation formation and delay wound healing. PMID: 27037022
  37. Klotho protein protects against diabetic nephropathy through multiple pathways. PMID: 26586006
  38. In vitro studies showed interleukin-12 regulation on Klotho, while Klotho also acted as an inhibitor on interleukin-12, indicating the potential of Klotho for preserving pancreatic beta-cell function in diabetes. PMID: 27330715
  39. Studies indicate that defect in Klotho gene expression results in a phenotype of premature aging. PMID: 27226196
  40. beta-Klotho participates in the regulation of the peptide transporters PEPT1 and PEPT2. PMID: 27941311
  41. Results show that Klotho is expressed in skeletal muscle and levels are reduced by smoking. PMID: 27021580
  42. FGF23 regulates osteopontin secretion indirectly by suppressing alkaline phosphatase transcription and phosphate production in osteoblastic cells, acting through FGF receptor-3 in a Klotho-independent manner PMID: 26235988
  43. Inhibition of mTOR signaling ameliorates vascular calcification via Klotho upregulation. PMID: 26061549
  44. Klotho deficiency promoted HFD-induced arterial stiffening and hypertension via downregulation of AMPKalpha activity. PMID: 26781278
  45. level of spontaneous remyelination was increased approximately two-fold in Klotho-overexpressing mice following cuprizone-induced demyelination. PMID: 26067431
  46. data highlight the importance of ASK1/p38 MAPK pathway in the brain and identify Klotho as a possible anti-oxidant effector PMID: 26452228
  47. Klotho ameliorates kidney injury and fibrosis and normalizes blood pressure by targeting the renin-angiotensin system. PMID: 26475416
  48. Injection of klotho protein results in hematopoietic changes opposite to the ones observed in Klotho(-/-) mice. PMID: 24412515
  49. Sodium bicarbonate administration has a favorable influence on vascular calcification and early death of klotho-deficient mice. PMID: 26538435
  50. Data suggest that the level of expression of Klotho in pancreatic beta-cells regulates apoptosis; low expression (halplodeficiency of Klotho) promotes apoptosis/type 1 diabetes; beta-cell-specific overexpression prevents apoptosis/type 1 diabetes. PMID: 26340932
  51. p16(INK4a) plays a previously unrecognized role in downregulating alpha-klotho expression during ageing. PMID: 25923845
  52. Klotho deficiency-induced arterial stiffening may involve aldosterone-mediated upregulation of scleraxis and induction of autophagy, which led to increased collagen-1 expression and decreased elastin levels, respectively. PMID: 26324504
  53. Melatonin attenuates oxidative stress and the associated memory impairment induced by klotho deficiency via signaling interaction between the MT2 receptor and ERK- and Nrf2-related antioxidant potential. PMID: 25550330
  54. Reduced KL expression in COPD airway epithelial cells was associated with increased oxidative stress, inflammation and apoptosis. PMID: 26201096
  55. alpha-klotho deficient mice have bone micro-fragility caused by the mimetic aging processes PMID: 25682161
  56. Data show that renal cells preferentially secrete klotho to the apical side and suggest that ADAMs are responsible for alpha-cut cleavage. PMID: 26155844
  57. The results suggested that the cardioprotective effects of klotho were related to the attenuation of oxidative stress, and oxidative stress suppressed the transcription of klotho PMID: 26093264
  58. Klotho deficient (kl/kl) mice exhibit Moncheberg's vascular calcification in the tunica media. PMID: 25926573
  59. aggravated cardiac hypertrophy observed in Klotho-deficient chronic kidney disease PMID: 25475745
  60. alpha-Klotho is a kind of acute phase protein. Serum levels are up-regulated in mice under restraint stress. PMID: 25337236
  61. Statin-mediated upregulation of klotho expression and differential regulation of FoxO expression promote resistance to CsA-induced oxidative stress. PMID: 22986347
  62. These results reveal that beta-cell-specific expression of Klotho improves beta-cell function and attenuates the development of type 2 diabetes mellitus. PMID: 25377875
  63. increasing wild-type klotho levels or activities improves synaptic and cognitive functions PMID: 25673831
  64. Alpha-Klotho expression is associated with expression of the non-sulfated HNK-1 epitope. PMID: 24723043
  65. Suggest that klotho does not play major role in the development of macrovascular disease in type 2 diabetes. PMID: 23945089
  66. KL deficiency causes salt-sensitive hypertension and renal damage by CCR2-mediated inflammation PMID: 24904083
  67. our study demonstrates that testosterone up-regulates anti-aging klotho together with AR expression in the kidney in vivo and in vitro by recruiting AR on to the AREs of the klotho promoter. PMID: 25163025
  68. FGF23 enhances phosphate-induced vascular calcification by promoting osteoblastic differentiation involving the ERK1/2 pathway in the absence of Klotho deficiency. PMID: 24088960
  69. These studies demonstrate a feedback loop between Klotho depletion and FGF2 activation in renal fibrosis. PMID: 25130652
  70. Klotho protects the kidney against cisplatin nephrotoxicity by reduction of basolateral uptake of cisplatin by OCT2 and a direct anti-apoptotic effect independent of cisplatin uptake. PMID: 24304882
  71. data indicate that immunolocalization of Klotho is closely correlated with proliferation in the intercalated cells of CNT and CCD from aging, and may be involved in the regulation of tubular proliferation. PMID: 24704992
  72. Early chronic kidney disease causes a reduction of vascular klotho. PMID: 23884339
  73. cardioprotection by klotho is related to the attenuation of ER stress and ER stress-induced apoptosis, at least partly, through suppressing activation of the p38 and JNK pathway PMID: 24340070
  74. Data suggest that klotho up-regulates secretion of growth hormone from pituitary and up-regulates expression of IGF-I (insulin-like growth factor I) and IGFBP-3 (insulin-like growth factor binding protein 3) in liver. PMID: 24939736
  75. Hyperlipidemia-associated renal damage decreases Klotho expression in kidneys from ApoE knockout mice. PMID: 24386260
  76. The presence of Klotho-independent FGF23 effects in a Klotho-expressing target organ represents a paradigm shift in the conceptualization of FGF23 endocrine action PMID: 24348262
  77. Results suggest an important link between klotho deficiency and muscle performance. PMID: 24030242
  78. Here we show that PAI-1 deficiency retards the development of senescence and protects organ structure and function while prolonging the lifespan of kl/kl mice. PMID: 24778222
  79. Klotho has a role in platelet Ca(2+) signaling and activation, an effect at least partially due to 1,25(OH)2D3-dependent down-regulation of NF-kappaB activity and STIM1/Orai1 expression in megakaryocytes PMID: 24522202
  80. It acts as a neuroprotective factor by modulating insulin-like growth factor 1 signaling and oxidative stress. PMID: 23973442
  81. Klotho is a novel player in the retina, with a clear connection to photoreceptor cell death as well as with an influence on retinal organization. PMID: 23796581
  82. In klotho mutant mice, inactivation of the JAK2/STAT3 signaling axis and M1 mAChR downregulation plays a critical role in cognitive impairment. PMID: 23389690
  83. Klotho is expressed in the retina and is important for healthy retinal function. Although the mechanisms for the observed abnormalities are not known, they are consistent with the accelerating aging phenotype seen in other tissues. PMID: 24045987
  84. Data from knockout mice suggest that klotho plays role in susceptibility to renal insufficiency in mice with diabetes type 1; mTOR (mammalian target of rapamycin) signal transduction in kidney is involved in exacerbation of diabetic nephropathy. PMID: 23928372
  85. We conclude that Klotho is an important regulator of retinal pigment epithelium homeostasis PMID: 24107965
  86. FGF23-Klotho signaling is absent in mouse arteries and that the vascular response was unaffected by FGF23 treatment PMID: 23577141
  87. Klotho deficiency upregulates NCKX1 membrane abundance and sodium-calcium-exchange activity, thus blunting the increase of calcium following lipoplysaccharide exposure and CCL12-mediated migration. PMID: 23596175
  88. Klotho(-/-) erythrocytes are particularly sensitive to osmotic shock, and enhanced eryptosis of klotho(-/-) erythrocytes is paralleled by enhanced adhesion to endothelial CXCL16. PMID: 23216570
  89. cardioprotection is mediated by downregulation of TRPC6 channels in the heart PMID: 23212367
  90. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. PMID: 22932118
  91. Levels of type IV collagen, a major component of basement membranes and a putative target of miR-29, were lower in klotho(-/-) and elderly ICR mice than in wild-type littermates and young ICR mice. PMID: 23139829
  92. FGF23 suppresses chondrocyte proliferation in the presence of soluble alpha-Klotho both in vitro and in vivo PMID: 23235154
  93. Klotho is a critical negative regulator of Wnt signaling and a suppressor of renal fibrosis in the obstructed kidney model. PMID: 23034937
  94. Alpha-klotho acts as an endocrine regulator of phosphate metabolism through controlling FGF23 production. PMID: 23187128
  95. Data demonstrated that a severe hypomorphic allele of Klotho had aberrant CpG methylation in kl/kl mice. PMID: 22782974
  96. Mice with partial deletion of Klotho in distal tubular segments were hyperphosphatemic with elevated FGF23 levels and abundant expression of the sodium-phosphate cotransporter Npt2a at the brush border membrane. PMID: 22878961
  97. demonstrate the first manifest evidence revealing that whereas alpha-Kl and beta-Kl are required for FGF23 and FGF15/hFGF19-mediated signaling pathways in vivo, respectively, beta-Kl appears not to be essential for FGF21-mediated signal transduction in vivo. PMID: 20080590
  98. vitamin D and Fgf23 independent function of Klotho in the regulation of glucose metabolism, bone turnover, and steady-state PTH secretion in vivo PMID: 22319626
  99. Renal fibrosis can trigger a deterioration spiral of Klotho expression, which may be involved in the pathophysiology of chronic kidney disease progression. PMID: 22338084
  100. alpha-Klotho expression was decreased in distal convoluted tubules in diabetic nephropathy in humans and mice. Renal loss of alpha-Klotho may affect urinary calcium excretion in early diabetic nephropathy. PMID: 22217880

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Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, Apical cell membrane, Single-pass type I membrane protein
Protein Families Glycosyl hydrolase 1 family, Klotho subfamily
Tissue Specificity Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed str
Database Links

KEGG: mmu:16591

STRING: 10090.ENSMUSP00000077899

UniGene: Mm.6500

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