Mouse Klotho ELISA Kit

Code CSB-E14362m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O35082
Lead Time 3-5 working days
Abbreviation KL
Target Name klotho
Alias N/A
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
HGNC 17080
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-90  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 92  
Range % 87-99  
1:8 Average % 95  
Range % 90-102  
Recovery
The recovery of mouse klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-104  
EDTA plasma (n=4) 98 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.157 2.107 2.132 1.968  
100 1.372 1.334 1.353 1.189  
50 0.805 0.798 0.802 0.638  
25 0.516 0.504 0.510 0.346  
12.5 0.348 0.331 0.340 0.176  
6.25 0.273 0.258 0.266 0.102  
3.12 0.228 0.215 0.222 0.058  
0 0.168 0.160 0.164    
References
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Function May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; FUNCTION
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, Apical cell membrane, Single-pass type I membrane protein
Protein Families Glycosyl hydrolase 1 family, Klotho subfamily
Tissue Specificity Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed str
Database Links

KEGG: mmu:16591

STRING: 10090.ENSMUSP00000077899

UniGene: Mm.6500

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